A5018131200- Lymphatic System and Diseases
- Glaucoma and retinal disorders
- Corneal surgery and disorders
- Lymphatic Disorders and Treatments
- Corneal Surgery and Treatments
- Diagnosis and Treatment of Venous Diseases
- Body Contouring and Surgery
- Intraocular Surgery and Lenses
- Planarian Biology and Electrostimulation
- Ion Transport and Channel Regulation
- Heart Rate Variability and Autonomic Control
- Trace Elements in Health
- Sympathectomy and Hyperhidrosis Treatments
- Heavy Metal Exposure and Toxicity
- Congenital Diaphragmatic Hernia Studies
- Health and Medical Research Impacts
- Voice and Speech Disorders
- Tracheal and airway disorders
- Vascular Malformations and Hemangiomas
- Neonatal Respiratory Health Research
- Ion channel regulation and function
- Cardiovascular Health and Disease Prevention
- Health Sciences Research and Education
- Dialysis and Renal Disease Management
- Cardiovascular and Diving-Related Complications
Aarhus University Hospital
2013-2025
Aarhus University
2011-2024
Telus (Canada)
2021
Ophthalmology Associates (United States)
2017
Background: The Fontan procedure has revolutionized the treatment of univentricular hearts. However, it is associated with severe complications such as protein-losing enteropathy, plastic bronchitis, and peripheral edema that may involve lymphatic circulation. We aimed to assess function morphology in patients a Methods: functional state vessels lower extremities circulation (n=10) was investigated using novel technique near-infrared fluorescence imaging compared an age-, sex-,...
Key points We studied the effects of antihypertensive calcium channel blockers on Ca V 1.2, predominantly expressed L‐type in largest human lymphatic vessel, thoracic duct. Human collecting vessels, both large and small, are highly‐sensitive vitro to blockers; exposure these drugs inhibits endogenous contractile activity action potentials diminishes noradrenaline‐induced phasic contractions. In vivo administration blocker nifedipine healthy volunteers did not reduce despite all subjects...
Abstract Purpose To evaluate the intraocular pressure (IOP) lowering effect and success rate of Paul glaucoma implant (PGI) in refractory after changing practice pattern from Ahmed Baerveldt tubes to PGI. Methods A prospective observational study first 50 consecutive PGI surgeries at a single Danish tertiary centre January 2022 October 2023. Primary endpoints were IOP rates 12 months. Secondary use IOP‐lowering medications complications. All cases had risk failure for traditional surgery...
The current study characterizes the mechanical properties of human thoracic duct and demonstrates a role for adrenoceptors, thromboxane, endothelin receptors in lymph vessel function. With ethical permission informed consent, portions (2-5 cm) were resected retrieved at T(7)-T(9) during esophageal cardia cancer surgery. Ring segments (2 mm long) mounted myograph isometric tension (N/m) measurement. diameter-tension relationship was established using ducts from 10 individuals. Peak active...
Key points Initial studies have described the electrical properties of lymphatic smooth muscle cells from different animal species and ion channels contributing to these properties. However, there is a translational gap human situation where on tissue are lacking. Voltage‐gated sodium essential for generation action potentials, thus spontaneous contractions in vessels, but not noradrenaline‐induced contractions. A channel opener elicited as result calcium influx via voltage‐gated...
In smooth muscle cells, K(+) permeability is high, and this highly influences the resting membrane potential. Lymph propulsion dependent on phasic contractions generated by cells of lymphatic vessels, it likely that channels play a critical role in regulating contractility tissue. The aim study was to investigate contribution distinct human vessel contractility. Thoracic ducts were harvested from 43 patients mounted wire myograph for isometric force measurements or potential recordings with...
Lymphatic vessels from animals have been shown to be innervated. While morphological studies confirmed human lymphatic are innervated, functional supporting this lacking. The present study demonstrates a innervation of the thoracic duct (TD) that is predominantly adrenergic. TDs harvested 51 patients undergoing esophageal and cardia cancer surgery were either fixed for structural investigations or maintained in vitro assessment by isometric force measurements electrical field stimulation...
Interstitial Cajal-like cells (ICLCs) are speculated to be pacemakers in smooth muscle tissues. While the human thoracic duct (TD) is spontaneously active, origin of this activity unknown. We hypothesized that ICLCs could present TD and using histological techniques, immunohistochemistry immunofluorescence we have investigated presence ICLCs, protein markers for cellular morphology TD. Transmission electron microscopy was employed investigate ultrastructure. Methylene blue staining,...
Background: Near-infrared fluorescence (NIRF) imaging is a new technique that used to visualize lymphatic vessels in humans. It has high spatial and temporal resolution, allowing real-time visualization of flow. Methods Results: The current study investigated the intra- inter-individual variability technique, how local hypo- hyperthermia (20°C, 40°C), as well exercise affect lymph transport. In this study, 10 healthy volunteers were studied twice, with 2 weeks between. NIRF was conducted by...
Human lymphatic vessels are myogenically active and respond to sympathetic stimulation. The role of various cations in this behavior has recently been investigated, but whether the anion Cl- is essential unclear. With ethical approval informed consent, human thoracic duct mesenteric were obtained from surgical patients. Spontaneous or norepinephrine-induced isometric force production isolated was measured by wire myography; transmembrane gradient channels investigated substitution...
The high blood pressure in giraffe leg arteries renders giraffes vulnerable to edema. We investigated 11 whether large and small the legs tight fascia protect capillaries. Ultrasound imaging of foreleg anesthetized ex vivo examination revealed abrupt thickening arterial wall a reduction its internal diameter just below elbow. At distal this narrowing, artery constricted spontaneously response norepinephrine intravascular recordings dynamic, viscous drop along artery. Histology isolated...
The aim of this study was to test the use BioCornea, a fish scale-derived collagen matrix for sealing full-thickness corneal perforations in mini-pigs. Two series experiments were carried out 8 Lan-Yu and 3 Göttingen mini-pigs, respectively. A 2mm central full thickness perforation made with surgical scissors trephines. sealed immediately by suturing BioCornea wounded cornea. conditions each patched cornea followed-up daily or 4 days. Status operated eyes assessed slit lamp examination...
Background: Mesenteric lymphatic vessels (MLVs) from various animal species have been intensively studied. We aimed to establish the viability and basic contractile characteristics of human MLVs maintained in vitro determine reactivity with norepinephrine (NE) substance P (SP) compare thoracic duct (TD). Methods Results: Isolated were mounted on a wire myograph under isometric conditions tension was recorded. The diameter–tension for generated by stretching stimulating 125 mM K+ solution...
Background: Knowledge of functional parameters that can be used for evaluation upper extremity lymphatic function is limited. This study aims to evaluate near-infrared fluorescence (NIRF) imaging in the human arm. Methods and Results: Ten healthy male volunteers (age 25.7 ± 1.3 years) were included examined at two occasions. The frequency velocity baseline, during hyperthermia after exercise. In addition, maximum pressure generated by contraction vessels was estimated. mean found 0.9...
To test the feasibility of implanting human anterior lens capsules (HALCs) with porcine corneal endothelial cells (pCEC) in vivo Göttingen minipigs and at same time suitability minipig as model for keratoplasty.Cell-carrier constructs decellularized HALC cultured were created implementation vivo. Eight (6 months old) underwent surgery descemetorhexis or removal endothelium by scraping without (animal 1-4) 5-8) pCEC. Follow-up examinations included optical coherence tomography (OCT) imaging...
Purpose: The purpose of this study was to investigate the use anterior segment imaging in diagnosing Kayser–Fleischer rings patients with Wilson disease. Methods: In a tertiary center for disease, were examined Pentacam HR Scheimpflug-based tomography device addition conventional slit-lamp examination. inferior part cornea analyzed using both built-in densitometry module and ImageJ. Results: Thirty-one disease (78% all Danish patients) included, resulting 83 examinations over 5-year period....
Background: The current belief is that the calcium channel blocker (CCB)-induced edema due to a preferential arterial over venous dilatation leading increased fluid filtration. We challenged this conviction by measuring lymphatic removal of interstitial during chronic systemic treatment with CCB, amlodipine. Lymphatic vessels could potentially be an off-target effect drugs and play role in CCB edema. Methods Results: Sixteen healthy postmenopausal women completed 12-week double-blinded...
The purpose of this study was to determine if conjunctival lymphangiogenesis can be induced using adenoviral delivery vascular endothelial growth factor C (VEGF-C).Seventeen New Zealand white rabbits received a subconjunctival injection containing 3.5 × 107 plaque-forming units an vector the gene-encoding VEGF-C (Ad-VEGF-C). contralateral eye used for control experiment (the same volume either saline or empty vector). After 2 weeks, animals were examined with trypan blue lymphangiography,...
Abstract Purpose To investigate circumpapillary retinal nerve fibre layer (RNFL) thickness, pupillary function and diameter after phacoemulsification lens implantation alone or combined with endothelial keratoplasty (EK). Methods This study was a secondary analysis of data from randomized, single‐masked trial, that included 72 patients Fuchs' dystrophy cataract, equally allocated (1:1) to ultrathin Descemet's stripping automated membrane keratoplasty. The cataract extraction (CE) group 40...