A5073731058- Plant tissue culture and regeneration
- Cancer Treatment and Pharmacology
- Plant Pathogens and Fungal Diseases
- Transgenic Plants and Applications
- Plant biochemistry and biosynthesis
- Medicinal Plants and Neuroprotection
- Microbial Natural Products and Biosynthesis
- Plant Molecular Biology Research
- Ginseng Biological Effects and Applications
- Essential Oils and Antimicrobial Activity
- Plant-Microbe Interactions and Immunity
- Plant-derived Lignans Synthesis and Bioactivity
- Fungal Biology and Applications
- Plant Gene Expression Analysis
- Phytochemicals and Antioxidant Activities
- Allelopathy and phytotoxic interactions
- Toxin Mechanisms and Immunotoxins
- Plant Genetic and Mutation Studies
- Cholinesterase and Neurodegenerative Diseases
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- GABA and Rice Research
- Chemical synthesis and alkaloids
- Seed Germination and Physiology
- Natural product bioactivities and synthesis
Universitat de Barcelona
2013-2022
Universidad Autónoma de Nuevo León
2019
FC Barcelona
2016-2017
In order to increase the production of pharmaceuticals hyoscyamine and scopolamine in hairy root cultures, a binary vector system was developed introduce T-DNA Ri plasmid together with tobacco pmt gene under control CaMV 35S promoter, into genome Datura metel Hyoscyamus muticus. This codes for putrescine:SAM N-methyltransferase (PMT; EC. 2.1.1.53), which catalyses first committed step tropane alkaloid pathway. Hairy cultures overexpressing aged faster accumulated higher amounts alkaloids...
Abstract Many plant‐derived agents are being used to treat cancer, including taxol, vinblastine, vincristine, or camptothecin and podophyllotoxin derivatives, among others. Plant biotechnology can provide a new tool for the production of anticancer but in spite considerable efforts produce vinblastine vincristine cell cultures knowledge biosynthetic pathway Catharanthus roseus alkaloids, biotechnological taxol has only been achieved at an industrial level by companies such as Phyton Biotech...
Summary Methyl jasmonate and cyclodextrins are proven effective inducers of secondary metabolism in plant cell cultures. Cyclodextrins, which cyclic oligosaccharides, can form inclusion complexes with nonhydrophilic products, thus increasing their excretion from the producer cells to culture medium. In present work, using a selected Taxus x media line cultured two‐stage system, relationship between taxane production transcript profiles several genes involved taxol was studied gain more...
Summary Grapevine stilbenes, particularly trans‐resveratrol, have a demonstrated pharmacological activity. Other natural stilbenes derived from resveratrol such as pterostilbene or piceatannol, display higher oral bioavailability and bioactivity than the parent compound, but are far less abundant in sources. Thus, to efficiently obtain these bioactive derivatives, there is need develop new bioproduction systems. cell cultures able produce large amounts of easily recoverable extracellular...
Plants remain a major source of new drugs, leads and fine chemicals. Cell cultures deriving from plants offer fascinating tool to study plant metabolic pathways large scale production systems for valuable compounds - commercial examples include such as paclitaxel. The constraint with undifferentiated cell is that they are generally considered be genetically unstable cultured cells tend produce low yields secondary metabolites especially over time. Hairy roots, tumor tissue caused by...
We tested the effect of three variables: bioreactor system (Wave or Spray reactor), medium exchange and culture period, on capacity a selected hairy root line Panax ginseng to produce ginsenosides. Among reactors, Wave appeared be most efficient in promoting growth. Periodic exchanges longer period increased growth rate cultured and, consequently, its Under established optimum conditions (medium every 14 days over 56 using bioreactor), initial fresh weight was enhanced more than 28-fold,...
Abstract Paclitaxel and baccatin III‐producing cells of Taxus baccata were immobilized within Ca 2+ ‐alginate beads. Under established optimum conditions for the biosynthesis both taxanes, yields paclitaxel III in shake‐flask cultures free increased by factors up to 3 2, respectively, corresponding cells. Although scale‐up from bioreactor culture usually results reduced productivities when grown same three different types (Stirred, Airlift, Wave) running 24 days a batch mode with system...
Dedifferentiated Taxus media cell cultures presenting the same genetic characteristics as parent culture were established from transformed roots. Two lines studied: Rol C, carrying T-DNA of A. rhizogenes 9,402 and TXS, both txs transgene T. baccata under control 35S CaMV promoter. In second part a previously optimized two-stage system, cultured in production medium supplemented with elicitor methyl jasmonate. Taxane was compared an untransformed line conditions. The highest taxane observed...
Taxol (paclitaxel) and its derivatives are microtubule-stabilizing drugs widely used in the treatment of several types cancer, including mammary, prostate, ovarian non-small-cell lung carcinoma, as well AIDS-associated Kaposi's sarcoma other tumor. Taxanes stabilize microtubules by enhancing their polymerization inhibiting depolymerization. Microtubule dynamics crucial to mitotic spindle formation function; therefore, cells exposed taxanes unable undergo chromosomal separation during...
The potent antimalarial drug artemisinin has a high cost, since its only viable source to date is Artemisia annua (0.01–0.8% DW). There therefore an urgent need design new strategies increase production or find alternative sources. In the current study, carvifolia Buch was selected with aim of detecting and then enhancing target compound derivatives. These metabolites were determined by LC-MS in shoots A. wild type plants at following concentrations: (8μg/g), artesunate (2.24μg/g),...