A5052395692- Plant tissue culture and regeneration
- Cancer Treatment and Pharmacology
- Plant Pathogens and Fungal Diseases
- Transgenic Plants and Applications
- Plant biochemistry and biosynthesis
- Medicinal Plants and Neuroprotection
- Microbial Natural Products and Biosynthesis
- Plant Molecular Biology Research
- Fungal Biology and Applications
- Ginseng Biological Effects and Applications
- Plant Gene Expression Analysis
- Plant-Microbe Interactions and Immunity
- Plant Genetic and Mutation Studies
- GABA and Rice Research
- Natural product bioactivities and synthesis
- Essential Oils and Antimicrobial Activity
- Plant nutrient uptake and metabolism
- Phytochemicals and Antioxidant Activities
- Seed Germination and Physiology
- Plant-derived Lignans Synthesis and Bioactivity
- Plant Parasitism and Resistance
- Chromosomal and Genetic Variations
- Photosynthetic Processes and Mechanisms
- Phytochemicals and Medicinal Plants
- Polyamine Metabolism and Applications
Universitat de Barcelona
2016-2025
Universidad Autónoma de Nuevo León
2019
FC Barcelona
2016-2017
Research Institute Hospital 12 de Octubre
2016
Hospital Universitario 12 De Octubre
2015
Instituto de Fisiología Vegetal
2015
Universitat de les Illes Balears
1992
In order to increase the production of pharmaceuticals hyoscyamine and scopolamine in hairy root cultures, a binary vector system was developed introduce T-DNA Ri plasmid together with tobacco pmt gene under control CaMV 35S promoter, into genome Datura metel Hyoscyamus muticus. This codes for putrescine:SAM N-methyltransferase (PMT; EC. 2.1.1.53), which catalyses first committed step tropane alkaloid pathway. Hairy cultures overexpressing aged faster accumulated higher amounts alkaloids...
Abstract Many plant‐derived agents are being used to treat cancer, including taxol, vinblastine, vincristine, or camptothecin and podophyllotoxin derivatives, among others. Plant biotechnology can provide a new tool for the production of anticancer but in spite considerable efforts produce vinblastine vincristine cell cultures knowledge biosynthetic pathway Catharanthus roseus alkaloids, biotechnological taxol has only been achieved at an industrial level by companies such as Phyton Biotech...
Summary Methyl jasmonate and cyclodextrins are proven effective inducers of secondary metabolism in plant cell cultures. Cyclodextrins, which cyclic oligosaccharides, can form inclusion complexes with nonhydrophilic products, thus increasing their excretion from the producer cells to culture medium. In present work, using a selected Taxus x media line cultured two‐stage system, relationship between taxane production transcript profiles several genes involved taxol was studied gain more...
Summary Grapevine stilbenes, particularly trans‐resveratrol, have a demonstrated pharmacological activity. Other natural stilbenes derived from resveratrol such as pterostilbene or piceatannol, display higher oral bioavailability and bioactivity than the parent compound, but are far less abundant in sources. Thus, to efficiently obtain these bioactive derivatives, there is need develop new bioproduction systems. cell cultures able produce large amounts of easily recoverable extracellular...
Abstract The use of nanotechnology and biotechnology to improve the production plant bioactive compounds is growing. Hyoscyamus reticulatus L. a major source tropane alkaloids with wide therapeutic use, including treatment Parkinson's disease calm schizoid patients. In present study, hairy roots were obtained from two‐week‐old cotyledon explants H. using A7 strain Agrobacterium rhizogenes . effects different concentrations signaling molecule nano‐zinc oxide (ZnO) (0, 50, 100 200 mg/L), three...
We tested the effect of three variables: bioreactor system (Wave or Spray reactor), medium exchange and culture period, on capacity a selected hairy root line Panax ginseng to produce ginsenosides. Among reactors, Wave appeared be most efficient in promoting growth. Periodic exchanges longer period increased growth rate cultured and, consequently, its Under established optimum conditions (medium every 14 days over 56 using bioreactor), initial fresh weight was enhanced more than 28-fold,...
Abstract Paclitaxel and baccatin III‐producing cells of Taxus baccata were immobilized within Ca 2+ ‐alginate beads. Under established optimum conditions for the biosynthesis both taxanes, yields paclitaxel III in shake‐flask cultures free increased by factors up to 3 2, respectively, corresponding cells. Although scale‐up from bioreactor culture usually results reduced productivities when grown same three different types (Stirred, Airlift, Wave) running 24 days a batch mode with system...