A5051712124- Plant tissue culture and regeneration
- Cancer Treatment and Pharmacology
- Plant Pathogens and Fungal Diseases
- Transgenic Plants and Applications
- Plant biochemistry and biosynthesis
- Medicinal Plants and Neuroprotection
- Microbial Natural Products and Biosynthesis
- Fungal Biology and Applications
- Ginseng Biological Effects and Applications
- GABA and Rice Research
- Plant Molecular Biology Research
- Plant-Microbe Interactions and Immunity
- Natural product bioactivities and synthesis
- Essential Oils and Antimicrobial Activity
- Phytochemicals and Medicinal Plants
- Plant-derived Lignans Synthesis and Bioactivity
- Cholinesterase and Neurodegenerative Diseases
- Plant Stress Responses and Tolerance
- Tannin, Tannase and Anticancer Activities
- Chromosomal and Genetic Variations
- Plant Genetic and Mutation Studies
- Plant nutrient uptake and metabolism
- Biochemical and biochemical processes
- Chemical synthesis and alkaloids
- Potato Plant Research
Universitat de Barcelona
2015-2024
Instituto de Fisiología Vegetal
2015
VTT Technical Research Centre of Finland
2004
Universitat Pompeu Fabra
2004
Second Military Medical University
2004
Fudan University
2004
Center for Life Sciences
2004
Southwest University
2004
Shanghai Jiao Tong University
2004
Institut des Sciences du Végétal
2003
Scopolamine is a pharmaceutically important tropane alkaloid extensively used as an anticholinergic agent. Here, we report the simultaneous introduction and overexpression of genes encoding rate-limiting upstream enzyme putrescine N-methyltransferase (PMT) downstream hyoscyamine 6 β-hydroxylase (H6H) scopolamine biosynthesis in transgenic henbane (Hyoscyamus niger) hairy root cultures. Transgenic lines expressing both pmt h6h produced significantly higher (P < 0.05) levels compared with...
Abstract Many plant‐derived agents are being used to treat cancer, including taxol, vinblastine, vincristine, or camptothecin and podophyllotoxin derivatives, among others. Plant biotechnology can provide a new tool for the production of anticancer but in spite considerable efforts produce vinblastine vincristine cell cultures knowledge biosynthetic pathway Catharanthus roseus alkaloids, biotechnological taxol has only been achieved at an industrial level by companies such as Phyton Biotech...
Summary Methyl jasmonate and cyclodextrins are proven effective inducers of secondary metabolism in plant cell cultures. Cyclodextrins, which cyclic oligosaccharides, can form inclusion complexes with nonhydrophilic products, thus increasing their excretion from the producer cells to culture medium. In present work, using a selected Taxus x media line cultured two‐stage system, relationship between taxane production transcript profiles several genes involved taxol was studied gain more...
Abstract Paclitaxel and baccatin III‐producing cells of Taxus baccata were immobilized within Ca 2+ ‐alginate beads. Under established optimum conditions for the biosynthesis both taxanes, yields paclitaxel III in shake‐flask cultures free increased by factors up to 3 2, respectively, corresponding cells. Although scale‐up from bioreactor culture usually results reduced productivities when grown same three different types (Stirred, Airlift, Wave) running 24 days a batch mode with system...
Dedifferentiated Taxus media cell cultures presenting the same genetic characteristics as parent culture were established from transformed roots. Two lines studied: Rol C, carrying T-DNA of A. rhizogenes 9,402 and TXS, both txs transgene T. baccata under control 35S CaMV promoter. In second part a previously optimized two-stage system, cultured in production medium supplemented with elicitor methyl jasmonate. Taxane was compared an untransformed line conditions. The highest taxane observed...
In this study, a total of 25 endophytic fungi were successfully isolated from the inner bark Taxus baccata grown in Iran by aseptic technique. Genomic DNA was extracted and subjected to polymerase chain reaction (PCR) analysis for presence taxadiene synthase (ts) gene, which encodes enzyme catalyzing first committed step taxol biosynthesis. Four isolates showed PCR positive ts gene. Subsequently, 10-deacetylbaccatin III (10-DAB III) culture filtrates mycelia analyzed high-performance liquid...
Taxol (paclitaxel) and its derivatives are microtubule-stabilizing drugs widely used in the treatment of several types cancer, including mammary, prostate, ovarian non-small-cell lung carcinoma, as well AIDS-associated Kaposi's sarcoma other tumor. Taxanes stabilize microtubules by enhancing their polymerization inhibiting depolymerization. Microtubule dynamics crucial to mitotic spindle formation function; therefore, cells exposed taxanes unable undergo chromosomal separation during...
The potent antimalarial drug artemisinin has a high cost, since its only viable source to date is Artemisia annua (0.01–0.8% DW). There therefore an urgent need design new strategies increase production or find alternative sources. In the current study, carvifolia Buch was selected with aim of detecting and then enhancing target compound derivatives. These metabolites were determined by LC-MS in shoots A. wild type plants at following concentrations: (8μg/g), artesunate (2.24μg/g),...