A5041553670- Microbial Natural Products and Biosynthesis
- Marine Sponges and Natural Products
- Fungal Biology and Applications
- Enzyme Catalysis and Immobilization
- X-ray Diffraction in Crystallography
- Carbohydrate Chemistry and Synthesis
- Crystallization and Solubility Studies
- Synthetic Organic Chemistry Methods
- Chemical Synthesis and Analysis
- Chemical synthesis and alkaloids
- Microbial Metabolic Engineering and Bioproduction
- Analytical Chemistry and Chromatography
- Plant Pathogens and Fungal Diseases
- Synthesis and Biological Activity
- Plant biochemistry and biosynthesis
- Steroid Chemistry and Biochemistry
- Microbial Community Ecology and Physiology
- Biochemical and Molecular Research
- Phytochemicals and Medicinal Plants
- Oxidative Organic Chemistry Reactions
- Plant-Microbe Interactions and Immunity
- Genomics and Phylogenetic Studies
- Traditional and Medicinal Uses of Annonaceae
- Algal biology and biofuel production
- Metabolomics and Mass Spectrometry Studies
Centre National de la Recherche Scientifique
2015-2025
Institut de Chimie des Substances Naturelles
2016-2025
Université Paris-Saclay
2020-2025
University of Puerto Rico at Carolina
2021
Université Paris-Sud
2020
University of Malaya
2010
Université Paris Cité
1987-1991
Laboratoire de Chimie et Biochimie Pharmacologiques et Toxicologiques
1987-1991
The beta-lactam antibiotics remain the most commonly used to treat severe infections. Because of structural similarity between ring and d-alanyl(4)-d-alanine(5) extremity bacterial cell wall precursors, drugs act as suicide substrates dd-transpeptidases that catalyze last cross-linking step assembly. Here, we show this mechanism action can be defeated by a novel type transpeptidase identified for first time reverse genetics in abeta-lactam-resistant mutant Enterococcus faecium. enzyme,...
The Zika virus (ZIKV), an emerging orthoflavivirus, presents a significant public health threat due to its rapid dissemination and association with severe neurological complications. urgent need for effective antiviral agents has driven research into novel bioactive compounds derived from unique natural sources. Microorganisms inhabiting extreme environments are particularly promising such discoveries their potential produce metabolites. In this study, we explored microorganisms the...
A Rhizobium sp. strain, named PATR, was isolated from an agricultural soil and found to actively degrade the herbicide atrazine. Incubation of PATR in a basal liquid medium containing 30 mg atrazine liter(sup-1) resulted rapid consumption accumulation hydroxyatrazine as only metabolite detected after 8 days culture. Experiments performed with ring-labeled [(sup14)C]atrazine indicated no mineralization. The enzyme responsible for hydroxylation partially purified consist four 50-kDa subunits....
Abstract Surfactins are a family of heptacyclopeptides in which the C‐terminal carbonyl is linked with β‐hydroxy group fatty acid acylating N‐terminal function glutamic residue. The acyl chain 12–16 carbon atoms long. These compounds, secreted by Gram‐positive bacterium Bacillus subtilis stationary phase liquid cultures, play an important role swarming communities on surface agar media formation dendritic patterns. TOF secondary ion MS (TOF‐SIMS) imaging was used to map surfactins within...
Parkinson's disease is a neurodegenerative proteinopathy that primarily affects mesencephalic dopaminergic neurons. This depletion can be phenotypically reproduced in various experimental models through the administration of two neurotoxins: N-methyl-4-phenylpyridinium (MPP+) and 6-hydroxydopamine (6-OHDA). The mechanisms underlying cell death processes induced by these toxins remain subject debate. In this context, studies suggest oxidative-stress-related may contribute to dysfunction...
Global warming has caused elevated seawater temperature and coral bleaching, including events on shallow reefs in the upper Gulf of Thailand (uGoT). Previous studies have reported an association between loss zooxanthellae bleaching. However, microbial diversity prokaryotes eukaryotes (microbiome) as holobionts are also important this information is still limited uGoT. To address shortcoming, report provided baseline prokaryotic (bacteria archaea) eukaryotic microbes healthy bleached colonies...
Summary The role of histone 3 lysine 4 (H3K4) methylation is poorly understood in plant pathogenic fungi. Here, we analysed the function CclA, a subunit COMPASS complex mediating H3K4 methylation, brassica anthracnose pathogen Colletotrichum higginsianum . We show that CclA required for full genome‐wide trimethylation. deletion cclA strongly reduced mycelial growth, asexual sporulation and spore germination but did not impair morphogenesis specialized infection structures (appressoria...
Aging research aims at developing interventions that delay normal aging processes and some related pathologies. Recently, many compounds extracts from natural products have been shown to and/or extend lifespan. Marine sponges their associated microorganisms found produce a wide variety of bioactive secondary metabolites; however, those the Southwest Indian Ocean are much less studied, especially regarding anti-aging activities. In this study, microbial diversity marine sponge Scopalina...
The bioremediation of aqueous wastes containing 5-nitro-1,2,4-triazol-3-one (NTO) was investigated. microorganism used is a Bacillus licheniformis strain, isolated from the contaminated solutions by enrichment techniques. biodegradation carried out in waste (15 g l−1 NTO) and proceeded through nitroreduction NTO, followed ring cleavage formed primary amine 5-amino-1,2,4-triazol-3-one (ATO). Both steps were optimized according to optimal conditions, NTO total 24 h, while degradation ATO...
Infection of Arabidopsis thaliana by the ascomycete fungus Colletotrichum higginsianum is characterized an early symptomless biotrophic phase followed a destructive necrotrophic phase. The fungal genome contains 77 secondary metabolism-related biosynthetic gene clusters, whose expression during infection process tightly regulated. Deleting CclA, chromatin regulator involved in repression some clusters through H3K4 trimethylation, allowed overproduction three families terpenoids and isolation...
Two novel α,β-unsaturated γ-lactono-hydrazides, geralcin A (2) and B (3), were isolated from Streptomyces sp. LMA-545. This unusual scaffold consists of the condensation alkyl-hydrazide with an γ-lactone, 3-(5-oxo-2H-furan-4-yl)propanoic acid (1), which was same broth culture. Amberlite XAD-16 solid-phase extraction used during cultivation step, trapped compounds (1–3) eluted resin methanol. The structures elucidated using 1H, 13C, 15N NMR spectroscopic analysis high-resolution mass...
Three novel hydrazides, geralcins C–E (1–3), were isolated from Streptomyces sp. LMA-545, together with MH-031 and A B. This unusual family of compounds was liquid-state agar-supported fermentation using Amberlite XAD-16 solid-phase extraction during the cultivation step. The use such neutral resin step allowed specific adsorption microbial secondary metabolites, avoiding any contamination crude extracts by constituents culture medium. trapped eluted methanol, their structures elucidated 1H,...
Two sporothriolide-related compounds were obtained from an extract of the fungus Hypoxylon monticulosum CLL-205, isolated a Sphaerocladina sponge collected Tahiti coast. Compound 2 is deoxy analogue sporothric acid (4). 3 newly reported unusual scaffold combining sporothriolide (1) and trienylfuranol A (5) moieties, through Diels–Alderase-type reaction. Various experimental analytical arguments supported biocatalytic origin compound 3. The structures elucidated using 1D 2D NMR, HRMS, IR...
Abstract Microbial specialized metabolite biosynthetic gene clusters (SMBGCs) are a formidable source of natural products pharmaceutical interest. With the multiplication genomic data available, very efficient bioinformatic tools for automatic SMBGC detection have been developed. Nevertheless, most these identify SMBGCs based on sequence similarity with enzymes typically involved in specialised metabolism and thus may miss coding undercharacterised enzymes. Here we present Synteruptor...
An efficient eight-step synthesis of optically pure (2S,3R,4S)-4-hydroxyisoleucine (1), a potent insulinotropic α-amino acid found in the seeds fenugreek (Trigonella foenum-graecum L.), is achieved 39% overall yield. The method suitable for large-scale production title compound. key steps involve biotransformation ethyl 2-methylacetoacetate to (2S,3S)-2-methyl-3-hydroxybutanoate (2) with Geotrichum candidum and an asymmetric Strecker synthesis. (© Wiley-VCH Verlag GmbH, 69451 Weinheim, Germany, 2002)