A5045640072- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- Bacteriophages and microbial interactions
- Advanced Fluorescence Microscopy Techniques
- Advanced Electron Microscopy Techniques and Applications
- Advanced Breast Cancer Therapies
- Near-Field Optical Microscopy
- Force Microscopy Techniques and Applications
- Gastrointestinal Tumor Research and Treatment
- RNA Interference and Gene Delivery
- Nanopore and Nanochannel Transport Studies
- DNA Repair Mechanisms
- ATP Synthase and ATPases Research
- Bacterial Genetics and Biotechnology
- FOXO transcription factor regulation
- Nanocluster Synthesis and Applications
- Cancer-related Molecular Pathways
- Cell death mechanisms and regulation
- Gastric Cancer Management and Outcomes
- Biochemical and Molecular Research
- Chronic Lymphocytic Leukemia Research
- Lung Cancer Research Studies
- Plasmonic and Surface Plasmon Research
- Single-cell and spatial transcriptomics
- Cell Image Analysis Techniques
Harvard University
2018-2020
University of California, Berkeley
2014-2018
Boston University
2015
Berkeley College
2014
University of Washington
2007-2008
Seattle University
2007
Abstract We present Light-Seq, an approach for multiplexed spatial indexing of intact biological samples using light-directed DNA barcoding in fixed cells and tissues followed by ex situ sequencing. Light-Seq combines spatially targeted, rapid photocrosslinking barcodes onto complementary DNAs with a one-step stitching reaction to create pooled, indexed sequencing libraries. This enables selection multiple cell populations tissue full-transcriptome based on location, morphology or protein...
Abstract Introduction Estrogen and insulin-like growth factor-1 (IGF-1) play important roles in mammary gland development breast cancer. SirT1 is a highly conserved protein deacetylase that can regulate the insulin/IGF-1 signaling lower organisms, as well growing number of transcription factors, including NF-κB, mammalian cells. Whether regulates IGF-1 for function, however, not clear. In present study, this role was examined by studying SirT1-deficient mice. Methods (SirT1 ko/ko ) mice were...
Bipolar disorder (BD) is a severe psychiatric condition marked by episodes of mania and depression, with neurotransmitter imbalance in the midbrain believed to play critical role its pathophysiology. Despite this, there currently no validated model for examining BD-associated molecular changes available. Leveraging recent advances stem cell technology, we developed organoid using human induced pluripotent cells (hiPSCs) from BD patients healthy controls (CTR). To address issues variability...
SpoIIIE is a homo-hexameric dsDNA translocase responsible for completing chromosome segregation in Bacillus subtilis. Here, we use single-molecule approach to monitor translocation when challenged with neutral-backbone DNA and non-hydrolyzable ATP analogs. We show that makes multiple essential contacts phosphates on the 5'→3' strand direction of translocation. Using constructs two segments separated by single charged base pair, deduce SpoIIIE's step size 2 bp. Finally, experiments analogs...
Prenatal exposure to low-dose radiation increases the risk of microcephaly and/or mental retardation. Microcephaly is also associated with genetic mutations that affect non-homologous end-joining pathway DNA double-strand break repair. To examine link between these two causal factors, we characterized neural developmental effects acute in mouse littermate embryos harboring Ku70 and p53 genes. Both deficiency induced morphologically indistinguishable cortical neuronal apoptosis. Irradiated...
Multi-subunit ring-shaped ATPases are molecular motors that harness chemical free energy to perform vital mechanical tasks such as polypeptide translocation, DNA unwinding, and chromosome segregation. Previously we reported the intersubunit coordination stepping behavior of hexameric ATPase SpoIIIE (Liu et al., 2015). Here use optical tweezers characterize motor’s mechanochemistry. Analysis motor response external force at various nucleotide concentrations identifies phosphate release likely...
Abstract Multi-subunit ring-shaped ATPases are molecular motors that harness chemical free energy to perform vital mechanical tasks such as polypeptide translocation, DNA unwinding, and chromosome segregation. Previously we reported the intersubunit coordination stepping behavior of hexameric ATPase SpoIIIE (Liu et al., 2015). Here use optical tweezers characterize motor’s mechanochemistry. Analysis motor response external force at various nucleotide concentrations identifies phosphate...
Abstract Gastrointestinal stromal tumor (GIST) is the most common GI sarcoma and generally initiated by KIT or PDGFRA mutations which are compelling therapeutic targets for tyrosine kinase inhibitors (TKI). However, emergence of secondary results in clinical resistance to available TKIs. GIST progression driven genomic events incrementally target p16-CDK4/6-RB1 p14-TP53-RB1 pathways create CDK4/6 CDK2 oncogenic co-dependency. Based on limited efficacy single-agent CDK4/6-inhibitor (CDK4/6i)...
Abstract Advanced GIST is characterized by genomic perturbations of key cell cycle regulators. Oncogenic activation CDK4/6 results in RB1 inactivation and progression. Given that single-agent inhibitor (CDK4/6i) therapy failed to show clinical activity advanced GIST, we evaluated strategies for maximizing response therapeutic inhibition. Targeted next-generation sequencing multiplexed protein imaging were used detect regulator aberrations samples (N=18), including 8 metastatic TKI-resistant...