A5068984259- Traumatic Brain Injury and Neurovascular Disturbances
- Traumatic Brain Injury Research
- Neuroscience and Neural Engineering
- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Nerve injury and regeneration
- S100 Proteins and Annexins
- Neuroscience and Neuropharmacology Research
- Neurogenesis and neuroplasticity mechanisms
- Cardiac Arrest and Resuscitation
- Metabolomics and Mass Spectrometry Studies
- Mesenchymal stem cell research
- EEG and Brain-Computer Interfaces
- Mitochondrial Function and Pathology
- Cell Adhesion Molecules Research
- Electrospun Nanofibers in Biomedical Applications
- Lipid Membrane Structure and Behavior
- Automotive and Human Injury Biomechanics
- Neural dynamics and brain function
- Tissue Engineering and Regenerative Medicine
- Dementia and Cognitive Impairment Research
- Mass Spectrometry Techniques and Applications
- Anesthesia and Neurotoxicity Research
- Ion channel regulation and function
- Cardiac electrophysiology and arrhythmias
The Wallace H. Coulter Department of Biomedical Engineering
2010-2025
Georgia Institute of Technology
2015-2025
Parker Hannifin (United States)
2012-2024
Emory University
2011-2023
San Francisco VA Health Care System
2020
Brain Injury Association of America
2019
University of Georgia
2019
Saint Xavier University
2019
Georgia Tech Research Institute
2016
University of Siena
2006
Abstract Traumatic injury to the central nervous system (CNS) triggers cell death and deafferentation, which may activate a cascade of cellular network disturbances. These events often result in formation irregularly shaped lesions comprised necrotic tissue and/or fluid‐filled cavity. Tissue engineering represents promising treatment strategy for injured neural tissue. To facilitate minimally invasive delivery engineered system, thermoreversible polymer is an attractive scaffold candidate....
The mechanism by which mechanical impact to brain tissue is transduced neuronal impairment remains poorly understood. Using an in vitro model of stretch, we found that stretch neurons resulted a transient plasma membrane permeability increase. Primary cortical neurons, seeded on silicone substrates, were subjected defined rate and magnitude strain pulse stretching the substrates over fixed cylindrical form. To identify defects, various sized fluorescent molecules added bathing media either...
Cell transplantation offers the potential to treat central nervous system injuries, largely because multiple mechanisms can be targeted in a sustained fashion. It is crucial that cells are transplanted into an environment favourable for extended survival and integration within host tissue. Given success of using fetal tissue grafts traumatic brain injury, it may beneficial mimic key aspects these (e.g. three-dimensionality, cell-cell cell-matrix support) deliver cells. Extracellular matrix...
Traumatic brain injury (TBI) is a major public health issue exacting substantial personal and economic burden globally. With the advent of "big data" approaches to understanding complex systems, there potential greatly accelerate knowledge about mechanisms how detect modify them improve patient outcomes. High quality, well-defined data are critical success bioinformatics platforms, dictionary "common elements" (CDEs), as well "unique has been created for clinical TBI research. There no...
We developed a new in vitro model of neuronal injury using NT2-N cells to examine the effects hydrodynamic loading rate on intraneuronal calcium dynamics and lactate dehydrogenase (LDH) release. Our apparatus consisted parallel disk viscometer which induced fluid shear stress with well-defined magnitudes rates cultured cells. found that deformation response was dependent severity insult, increased cellular strains generated for higher stresses at constant rate. Peak intracellular free...
Multipotential stem cells are an attractive choice for cell therapy after traumatic brain injury (TBI), as replacement of multiple types may be required functional recovery. In the present study, neural (NSCs) derived from germinal zone E14.5 GFP-expressing mouse brains were cultured neurospheres in FGF2-enhanced medium. When FGF2 was removed vitro, NSCs expressed phenotypic markers neurons, astrocytes, and oligodendrocytes exhibited migratory behavior presence adsorbed fibronectin (FN)....
Morphological and electrophysiological properties of neural cells are substantially influenced by their immediate extracellular surroundings, yet the features this environment difficult to mimic in vitro. Therefore, there is a tremendous need develop new generation culture systems that more closely model complexity nervous tissue. To end, we engineered novel electrophysiologically active 3D constructs composed neurons astrocytes within bioactive matrix-based scaffold. Neurons these exhibited...
Traumatic brain injury (TBI) results from cell dysfunction or death following supra-threshold physical loading. Neural plasmalemma compromise has been observed traumatic neural insults; however, the biomechanical thresholds and time-course of such disruptions remain poorly understood. In order to investigate trauma-induced membrane disruptions, we induced dynamic strain fields (0.50 shear compressive at 1, 10, 30?sec(?1) rate) in 3-D neuronal-astrocytic co-cultures (>500??m thick)....
Despite the large number of promising neuroprotective agents identified in experimental traumatic brain injury (TBI) studies, none has yet shown meaningful improvements long-term outcome clinical trials. To develop recommendations and guidelines for pre-clinical testing pharmacological or biological therapies TBI, Moody Project Translational Traumatic Brain Injury Research hosted a symposium attended by investigators with extensive experience TBI testing. The participants discussed issues...
Abstract Background Traumatic brain injury (TBI) results in irreversible damage at the site of impact and initiates cellular molecular processes that lead to secondary neural surrounding tissue. We used microarray analysis determine which genes, pathways networks were significantly altered using a rat model TBI. Adult rats received unilateral controlled cortical (CCI) sacrificed 24 h post-injury. The ipsilateral hemi-brain tissue injury, corresponding contralateral tissue, naïve (control)...
The nuclear enzyme poly(ADP-ribose) polymerase (PARP), which has been shown to be activated following experimental traumatic brain injury (TBI), binds DNA strand breaks and utilizes nicotinamide adenine dinucleotide (NAD) as a substrate. Since consumption of NAD may deleterious recovery in the setting CNS injury, we examined effect potent PARP inhibitor, GPI 6150, on histological outcome TBI rat. Rats (n = 16) were anesthetized, received preinjury dose 6150 (30 min; 15 mg/kg, i.p.),...
Many cellular models of traumatic brain injury (TBI) deform cells in a planar (2-D) configuration, contrast from the three-dimensional (3-D) architecture brain, resulting strain fields that may fail to represent complex deformation patterns seen vivo. Cells cultured 3-D more accurately vivo behavior than due differences cytostructure, cell-cell/cell-matrix interactions and access trophic factors; however, effects culture configuration on response high rate have not been evaluated. We...
Electrical activity is the ultimate functional measure of neuronal tissue and recording that remains a key technical challenge in neuroscience. The mechanical mismatch between rigid electrodes compliant brain critical limitation applications where movement an inherent component. An electrode permits neural activity, while minimizing disruption, beneficial for encompass both normal physiological movements those which require consistent during large displacements. In order to test extreme this...
Abstract This study tested the hypothesis that controlled flow through microchannels can cause shear‐induced intracellular loading of cells with molecules. The overall goal was to design a simple device expose fluid shear stress and thereby increase plasma membrane permeability. DU145 prostate cancer were exposed in presence fluorescent cell‐impermeant molecules by using cone‐and‐plate shearing or high‐velocity microchannels. Using syringe pump, cell suspensions flowed 50–300 µm diameter...
Biomaterial matrices presenting extracellular matrix (ECM) components in a controlled three-dimensional configuration provide unique system to study neural stem cell (NSC)–ECM interactions. We cultured primary murine neurospheres methylcellulose (MC) scaffold functionalized with laminin-1 (MC-x-LN1) and monitored NSC survival, apoptosis, migration, differentiation, production. Overall, MC-x-LN1 enhanced both survival maturation compared MC controls. Significantly lower levels of apoptotic...