A5070522317- Platelet Disorders and Treatments
- Blood properties and coagulation
- SARS-CoV-2 and COVID-19 Research
- Biotin and Related Studies
- Blood groups and transfusion
- Erythrocyte Function and Pathophysiology
- ATP Synthase and ATPases Research
- COVID-19 Clinical Research Studies
- Complement system in diseases
- Genetic Syndromes and Imprinting
- SARS-CoV-2 detection and testing
- Mitochondrial Function and Pathology
- Vascular Malformations and Hemangiomas
Erasmus MC
2023-2024
Agostino Gemelli University Polyclinic
2023
Istituti di Ricovero e Cura a Carattere Scientifico
2023
Università Cattolica del Sacro Cuore
2023
Rotterdam University of Applied Sciences
2022
Amsterdam University Medical Centers
2022
University of Amsterdam
2022
Endothelial cells, forming a monolayer along blood vessels, intricately regulate vascular hemostasis, inflammatory responses, and angiogenesis. A key determinant of these functions is the controlled secretion Weibel-Palade bodies (WPBs), which are specialized endothelial storage organelles housing presynthesized pool hemostatic protein von Willebrand factor various other hemostatic, inflammatory, angiogenic, vasoactive mediators. This review delves into recent mechanistic insights WPB...
Von Willebrand factor (VWF) and VWF propeptide (VWFpp) are stored in eccentric nanodomains within platelet alpha-granules. VWFpp can undergo differential secretion following Weibel-Palade body exocytosis endothelial cells; however, it is unclear if the same process occurs during alpha-granule exocytosis. Using a high-throughput 3-dimensional super-resolution imaging workflow for quantification of individual cargo, we studied cargo release response to different physiological stimuli.To...
Healthcare workers (HCWs) are at risk for coronavirus disease 2019 (COVID-19), and spreading severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) amongst colleagues patients.To study the presence of SARS-CoV-2 RNA possible onward transmission by HCWs upon return to work after COVID-19, association with severity development antibodies over time.Unvaccinated positive reverse transcriptase polymerase chain reaction (RT-PCR) were recruited prospectively. Data on symptoms collected via...
Abstract Background Platelet alpha-granules contain Von Willebrand factor (VWF), which is stored in eccentric alpha-granule nanodomains, and VWF propeptide (VWFpp). Differential release of VWFpp has been reported from endothelial cells. It unclear if this also occurs during platelet exocytosis. We have recently developed a 3D super-resolution imaging workflow for quantification content based on Structured Illumination Microscopy (SIM). With we can study cargo following activation hundreds...