A5034776627- Analytical Chemistry and Chromatography
- Microfluidic and Capillary Electrophoresis Applications
- Mass Spectrometry Techniques and Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Chromatography in Natural Products
- Analytical Methods in Pharmaceuticals
- Analytical chemistry methods development
- Insect-Plant Interactions and Control
- Pharmaceutical and Antibiotic Environmental Impacts
- Electrospun Nanofibers in Biomedical Applications
- Hemiptera Insect Studies
- Advanced Chemical Sensor Technologies
- Effects and risks of endocrine disrupting chemicals
- Cancer therapeutics and mechanisms
- Animal Behavior and Reproduction
- Glycosylation and Glycoproteins Research
- Molecular spectroscopy and chirality
- Antibiotics Pharmacokinetics and Efficacy
- Pesticide Residue Analysis and Safety
- Carbohydrate Chemistry and Synthesis
- Amphibian and Reptile Biology
- Sperm and Testicular Function
- Spectroscopy and Chemometric Analyses
- Graphene and Nanomaterials Applications
- Biosensors and Analytical Detection
Charles University
2015-2024
Czech Academy of Sciences, Institute of Analytical Chemistry
2001
Reptiles use pterin and carotenoid pigments to produce yellow, orange, red colors. These conspicuous colors serve a diversity of signaling functions, but their molecular basis remains unresolved. Here, we show that the genomes sympatric color morphs European common wall lizard ( Podarcis muralis ), which differ in orange yellow pigmentation ecology behavior, are virtually undifferentiated. Genetic differences restricted two small regulatory regions near genes associated with [ sepiapterin...
Nanofibers were prepared from polycaprolactone, polylactide and polyvinyl alcohol using Nanospider TM technology. Polyethylene glycols with molecular weights of 2 000, 6 10 000 20 g/mol, which can be used to moderate the release profile incorporated pharmacologically active compounds, served as model molecules. They terminated by aromatic isocyanate into nanofibers. The these molecules an aqueous environment was investigated. influences length chemical composition nanofibers on rate amount...
Abstract: Polyvinyl alcohol nanofibers incorporating the wide spectrum antibiotic gentamicin were prepared by Nanospider™ needleless technology. A polyvinyl layer, serving as a drug reservoir, was covered from both sides polyurethane layers of various thicknesses. The multilayered structure observed using scanning electron microscopy, porosity characterized mercury porosimetry, and nitrogen adsorption/desorption measurements used to determine specific surface areas. stability released...
A multi-residue UHPLC–MS/MS analytical method, previously developed for monitoring 52 pharmaceuticals in drinking water, was used to analyse these wastewater originating from healthcare facilities the Czech Republic. Furthermore, methodology expanded include evaluation of effectiveness drug removal treatment plants (WWTPs). Of 18 samples analysed by validated UHPLC-MS/MS, each sample contained at least one quantifiable analyte. This study reveals prevalence several different drugs; mean...
Topotecan (TPT) is used in the treatment of retinoblastoma, most common malignant intraocular tumor children. TPT undergoes pH-dependent hydrolysis lactone ring to ring-opened carboxylate form, with form showing antitumor activity. A selective, and highly sensitive ultra-high-performance liquid chromatography-tandem mass spectrometry method was developed for determination both forms one mobile phase composition plasma vitreous humor matrices. The showed an excellent linear range 0.375-120...
Animal body coloration is a complex trait resulting from the interplay of multiple mechanisms. While many studies address functions animal coloration, mechanisms colour production still remain unknown in most taxa. Here we compare reflectance spectra, cellular, ultra- and nano-structure colour-producing elements, pigment types two freshwater turtles with contrasting courtship behaviour, Trachemys scripta Pseudemys concinna . The species differ distribution cell-types diversity. We found...
(1) The occurrence and accumulation of pharmaceuticals personal care products in the environment are recognized scientific concerns. Many these compounds disposed an unchanged or metabolized form through sewage systems wastewater treatment plants (WWTP). WWTP processes do not completely eliminate all active substances their metabolites. Therefore, they systematically leach into water system increasingly contaminating ground, surface, drinking water, representing a health risk largely ignored...
Abstract Cyclofructans (CFs), a new class of chiral selectors, have been recently introduced for application in liquid chromatography and capillary electrophoresis. So far, derivatized CFs performed interesting separation possibilities variety compounds. The current work is focused on characterization three different CF‐based stationary phases (CF‐based CSPs), i.e. isopropyl carbamate cyclofructan 6 (IP‐CF6), R ‐naphthylethyl (RN‐CF6) dimethylphenyl 7 (DMP‐CF7). linear free energy...
Theory suggests that aposematism, specifically the learned avoidance of unprofitable prey via memorable color patterns, should result in selection for pattern uniformity. However, many examples to contrary are seen nature. Conversely, honest sexual signals likely exhibit greater variation because they reflect underlying mate quality. Here we aim characterize and quantify mechanistic causes Tectocoris diopthalmus shed light on costs production, thus potential information content its signals....
Cholesteryl esters of ω-(O-acyl)-hydroxy FAs (Chl-ωOAHFAs) were identified for the first time in vernix caseosa and characterized using chromatography MS. Chl-ωOAHFAs isolated adsorption on silica gel magnesium hydroxide. Their general structure was established high-resolution tandem MS intact lipids, products their transesterification derivatizations. Individual molecular species nonaqueous reversed-phase HPLC coupled to atmospheric pressure chemical ionization. The analytes detected as...
Abstract Four methacrylate ester‐based monolithic columns for capillary liquid chromatography (CLC) were prepared by radical polymerization with ammonium peroxodisulfate (3 columns) and thermal initiation (1 column). The mixture consisted of butyl (BMA) ethylene glycol dimethacrylate (EDMA), propan‐1‐ol, butane‐1,4‐diol, water, as initiator. It was necessary to add N,N,N ′, N ′‐tetramethylethylenediamine (TEMED) the activate reaction. amount initiator activator optimized attain quantitative...