Wwtr1 is a widely expressed 14-3-3-binding protein that regulates the activity of several transcription factors involved in development and disease. To elucidate physiological role Wwtr1, we generated Wwtr1-/- mice by homologous recombination. Surprisingly, although known to regulate Cbfa1, factor important for bone development, show only minor skeletal defects. However, animals present with renal cysts lead end-stage Cysts predominantly originate from dilation Bowman's spaces atrophy...

Pulses play a significant role in nitrogen cycling as they fix atmospheric N2 through symbiosis. However, it is unknown whether there are differences the ability of biological fixation (BNF) among pulse species and individual cultivars. Here, we quantified BNF selected cultivars determined effect on crop yield. A total 25 species-cultivar combinations chickpea (Cicer arietinum L.), dry bean (Phaseolus vulgaris faba (Vicia field pea (Pisum sativum lentil (Lens culinaris Medik.) were tested...

The plastic elongation of mesocotyl (MES) and coleoptile (COL), which can be repressed by light exposure, plays a vital role in maize seedling emergence establishment under adverse environmental conditions. Understanding the molecular mechanisms light-mediated repression MES COL will allow us to develop new strategies for genetic improvement these two crucial traits maize. A variety, Zheng58, was used monitor transcriptome physiological changes response darkness, as well red, blue, white...

A chickpea health issue was first noted in July 2019 southwest Saskatchewan, Canada. Surveys of commercial fields were undertaken 2021, 2022 and 2023 to investigate the potential roles herbicides, fungicides, fertilizer, seed treatments, inoculants, nutrients, nematodes, drought stress, cultivars on severity plant damage. Fields which herbicide containing active ingredient metribuzin had been applied more severe symptoms compared it not. Seed treatment with Apron Advance (active ingredients...

Detecting malicious or adversarial images, for example in security and surveillance systems, is an important problem computer vision. These results highlight the effectiveness of ViTs when compared to CNNs confronting hostile images. However, have stiff competition from been go-to architecture image classification object detection many years, due existence spatial hierarchies Using benchmark datasets containing a combination clean this study compares ability both models (i) detect (ii)...

Cyber bullying in close-contact environments, especially schools, is another huge social problem that has a deep impact on victims psychologically and mentally. Recent developments learning remote monitoring technologies offer the potential to improve real-time detection intervention strategies. In this work, we explore effectiveness of neural networks (DNNs) for classifying identifying trigger events events. Using spatial-temporal video data multimodal sensor inputs, present hierarchical...

Abstract The tropical pasture grass Brachiaria humidiola (Rendle) Schweick releases nitrification inhibitory compounds from its roots, a phenomenon termed 'biological inhibition' (BNI). We investigated the influence of root exudates B. humidicola on nitrification, major soil microorganisms and plant growth promoting using two contrasting types, Andosol Cambisol. addition (containing BNI activity that is expressed in Allylthiourea unit (ATU) was standardized bioassay against synthetic...

The association of plants and microbial communities is crucial for crop production, host influence the composition rhizosphere microbiomes. Pulse crops play an important role in development sustainable cropping systems, producers Canadian prairies often increase frequency pulses their systems. In this study, we determined shifts fungal community pea (Pisum sativum L.) rhizosphere, as influenced by rotation, using high throughput sequencing. Six systems containing (P), lentil (Lens culinaris...

The eukaryotic translation elongation factor eEF-1Bβ1 (EF1Bβ) is a guanine nucleotide exchange that plays an important role in elongation. In this study, we show the EF1Bβ protein localized plasma membrane and cytoplasm, transcripts should be expressed most tissue types seedlings. Sectioning of inflorescence stem revealed predominantly localizes to xylem vessels interfascicular cambium. gene silencing efβ caused dwarf phenotype with 38% 20% reduction total lignin crystalline cellulose,...

Brassica carinata A. Braun seeds contain high erucic and linolenic acids that are desirable for biofuel biopolymer products. However, key agronomic practices to maximize productivity on the Northern Great Plains not well known. Here, we investigated response of seed yield quality three factors—herbicide, seeding rate, N. The factorial experiment, conducted in nine environments (site‐years) Canadian prairies from 2014 2016, included two weed control options (weedy vs. weed‐controlled), levels...

Replication Protein A (RPA) is a broadly conserved complex comprised of the RPA1, 2 and 3 subunits. RPA protects exposed single-stranded DNA (ssDNA) during replication repair. Using structural modeling, we discover an inhibitor, JC-229, that targets RPA1 in Trypanosoma brucei, causative parasite African trypanosomiasis. The inhibitor highly toxic to T. brucei cells, while mildly human cells. JC-229 treatment mimics effects TbRPA1 depletion, including inhibition damage accumulation. In-vitro...

Serotonin (5-HT), an indoleamine compound, has been known to mediate many physiological responses of plants under environmental stress. The deep-seeding (≥20 cm) maize seeds is important cultivation strategy ensure seedling emergence and survival drought However, the role 5-HT in tolerance remains unexplored. Understanding mechanisms evaluating optimal concentration alleviating stress could benefit production. In this study, two inbred lines were treated with or without at both sowing depths...

Aphanomyces euteiches causes root rot in pea, leading to significant yield losses. However, the metabolites involved this pathosystem have not been thoroughly studied. This study aimed fill gap and explore mechanisms of bacterial suppression A. via untargeted metabolomics using pea grown a controlled environment. Chemical isotope labeling (CIL), followed by liquid chromatography-mass spectrometry (LC-MS), was used for metabolite separation detection. Univariate multivariate analyses showed...

Biological N 2 -fixation underpins the role of pulse crops in development sustainable cropping systems, but it is uncertain how nodulation and accumulation may differ with species, cultivars, environments. This 3 yr field study investigated at early late flowering stages seed straw uptake for chickpea (Cicer arietinum L.), dry bean (Phaseolus vulgaris faba (Vicia pea (Pisum sativum lentil (Lens culinaris Medik.). At flowering, all pulses except had more nodules wetter (2010) than drier year...

Brassica crops are cultivated widely for human consumption and animal feed purposes, oilseed rape/canola (Brassica napus rapa) is the second most important worldwide. Because of its natural diversity genetic complexity, genomics studies on rape will be a useful resource base to modify quantity quality biomass in various crops, therefore, should have positive impact lignocellulosic biofuel production. The objective this study was perform microarray analysis two variable lignin containing...

Processed plant-based foods, particularly high carbohydrate-containing are among the greatest contributors to dietary acrylamide, a probable human carcinogen, uptake. Between 2009 and 2020, five surveys were conducted determine acrylamide in foods Canada. These included sampling of potato sweet chips, French fries, frozen potato/sweet products, as follow-up our earlier from 2002 - 2008. Samples analyzed using isotope dilution (13C3-acrylamide) with LC-MS/MS. The highest mean levels found...