ABSTRACT C-phycocyanin (C-PC), a natural blue dye, has been used in food, immunodiagnostics and analytical reagents. In this paper, the optimization of two-stage extraction C-PC from Spirulina platensis cells was studied. First, biomass underwent different treatments for cell disruption, including drying, freezing milling at levels, then carried out using fixed time, impeller rotational speed biomass-to-solvent ratio. Having defined rupture conditions, process optimized, evaluating effects...

Carotenoids are natural pigments that can be produced through biotechnological processes. However, the costs relatively high and minimized by using lower-cost substrates as alternative nutrient sources. The fed-batch fermentation is one of techniques used to obtain a biomass concentration and/or maximum production. Thus, aim this work produce carotenoids in batch with yeast Rhodotorula mucilaginosa CCT 7688 agroindustrial byproducts culture medium. Carotenoid production was increased...

Abstract The production of lipase by Geotrichum candidum in both, stirred tank and airlift bioreactors were compared. G an imperfect filamentous fungus, grows well liquid medium, produces a with specific affinity for long‐chain fatty acids cis ‐9 double bonds but, is generally not efficient because the optimum medium composition fermentation conditions are known. Response surface methodology was used to optimize agitation speed (100–500 rpm) aeration (0.2–1.8 vvm) bench‐scale fermenter. A...

Astaxanthin (3,3'-dihydroxy-b,b'-carotene-4,4'-dione) is carotenoid of high market value whose demand has increased in such fields as aquaculture, pharmaceutical supplements and natural coloring. Cell disruption the first step for isolating intracellular materials it depends on cell wall permeability. In order to maximize recovery astaxanthin from Phaffia rhodozyma NRRL-Y17268, drying freeze pretreatments were tested by different methods: abrasion with celite, glass pearls vortex agitator,...

Different yeast strains from forests located in southern Brazil, with potential to produce carotenoids, were isolated. Three microorganisms selected as carotenoid producers. Sporiodiobolus pararoseus, Rhodotorula mucilaginosa and Pichia fermentans grown Yeast Malt (YM) medium the carotenoids produced identified cryptoxanthin β-carotene. In order reduce production costs, agroindustrial residues used formulation of A (parboiled rice water crude glycerol) B sugar cane molasses). The highest was...

This study aimed to evaluate the influence of magnetic fields (MF) on inoculum cultivation and carotenoid production by Phaffia rhodozyma. The application MF in culture was evaluated (0 m T – control 30 T). Cellular concentration increased 12.8 % after 24 h-culture with compared assay, this best alternative for preparation inoculum. Different intervals were over 168 h. highest volumetric carotenoids achieved applying throughout cultivation, values 1146.39 ± 26.18 μg L−1 productivity 11.94...

Carotenoids are pigments that can be applied to food but they unstable towards certain intrinsic conditions, as well processing ones.Microencapsulation is an alternative increasing their stability.This study aimed produce carotenoids by Phaffia rhodozyma crops and promote microencapsulation lyophilization with soy protein the wall material, in different proportions.High process yield of 96% encapsulation efficiency around 65% were observed at ratios under study.Well-defined separate...

The interest in carotenoid production from natural sources has increased based their possible use as dyes and powerful biological antioxidant capacity. This study evaluated the of rice parboiling wastewater (RPW) an alternative substrate for bioproduction carotenoids using yeast Phaffia rhodozyma found it to be promising only source nutrients, reaching a concentration 0.6 μg mL-1 (259.1 g-1) 48 h. To increase potential this industrial effluent, supplementation was carried out enhance...

In the present study, an innovative cultivation strategy of Rhodotorula mucilaginosa CCT 7688 to accumulate both lipids and high added value carotenoids was proposed, with potential contribute economic feasibility microbial oils in comparison vegetable oils. The stepwise fed-batch crude glycerol feeding at 24 h, 96 144 addition magnesium initial culture medium, resulted a lipid content 51.0 ± 0.3 % CDW, biomass concentration 21.00 0.48 g L-1, total production 10.72 0.18 productivity 0.037...

In a single-stage process with the simultaneous addition of β-galactosidase and lactic culture, lactose conversion, processing time, viscosity syneresis were evaluated. Fermentation was promoted by culture containing two probiotic microorganisms, Bifidobacterium animalis Lactobacillus acidophilus, associated typical microorganisms yogurt. An enzymatic preparation β-galactosidases from Kluyveromyces lactis Aspergillus niger used. A central composite design (2³ trials plus three points)...