Anna-Lena B.G. Vigil
A5076673665- Cancer, Lipids, and Metabolism
- Cancer, Hypoxia, and Metabolism
- Biological Research and Disease Studies
- Folate and B Vitamins Research
- Prostate Cancer Treatment and Research
- Metabolism, Diabetes, and Cancer
- Cancer Research and Treatments
- Amino Acid Enzymes and Metabolism
- Plant Molecular Biology Research
- Computational Drug Discovery Methods
- Single-cell and spatial transcriptomics
- Cancer-related Molecular Pathways
- Metabolomics and Mass Spectrometry Studies
- Epigenetics and DNA Methylation
- Enzyme Structure and Function
- S100 Proteins and Annexins
- Radiopharmaceutical Chemistry and Applications
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Machine Learning in Bioinformatics
- Nutrition, Genetics, and Disease
- Metabolism and Genetic Disorders
- Peptidase Inhibition and Analysis
- T-cell and B-cell Immunology
- Advanced Proteomics Techniques and Applications
- Immune Cell Function and Interaction
Fred Hutch Cancer Center
2022-2024
European Molecular Biology Laboratory
2024
Cape Town HVTN Immunology Laboratory / Hutchinson Centre Research Institute of South Africa
2023
Seattle University
2023
University of Nevada, Las Vegas
2020-2021
Abstract The scarcity of accessible sites that are dynamic or cell type-specific in plants may be due part to tissue heterogeneity bulk studies. To assess the effects heterogeneity, we apply single-cell ATAC-seq Arabidopsis thaliana roots and identify thousands differentially sites, sufficient resolve all major types root. We find entirety a cell’s regulatory landscape its transcriptome independently capture type identity. leverage this shared information on identity integrate accessibility...
The oxidative tricarboxylic acid (TCA) cycle is a central mitochondrial pathway integrating catabolic conversions of NAD +to NADH and anabolic production aspartate, key amino for cell proliferation. Several TCA components are implicated in tumorigenesis, including loss-of-function mutations subunits succinate dehydrogenase (SDH), also known as complex II the electron transport chain (ETC), but mechanistic understanding how proliferating cells tolerate metabolic defects SDH loss still...
Abstract Metastatic castration-resistant prostate cancer remains incurable regardless of recent therapeutic advances. Prostate tumors display highly glycolytic phenotypes as the progresses. Nonspecific inhibitors glycolysis have not been utilized successfully for chemotherapy, because their penchant to cause systemic toxicity. This study reports preclinical activity, safety, and pharmacokinetics a novel small-molecule candidate, BKIDC-1553, with antiglycolytic activity. We tested large...
Abstract In plants, chromatin accessibility – the primary mark of regulatory DNA is relatively static across tissues and conditions. This scarcity accessible sites that are dynamic or tissue-specific may be due in part to tissue heterogeneity previous bulk studies. To assess effects heterogeneity, we apply single-cell ATAC-seq A. thaliana roots identify thousands differentially sites, sufficient resolve all major cell types root. However, even this vast increase relative studies number does...
Placentation presents immune conflict between mother and fetus, yet in normal pregnancy maternal immunity against infection is maintained without expense to fetal tolerance. This believed result from adaptations at the maternal-fetal interface (MFI) which affect T cell programming, but identities (i.e., memory subsets antigenic specificities) of cells signals that mediate fates functions MFI remain poorly understood. We found intact recruitment programs as well pro-inflammatory cytokine...
<p>Table S2 shows rat cardiovascular assessment (nonGLP) after IV administration of increasing dosages BKIDC-1553.</p>
<p>Table S6 shows in vitro AMPK activity.</p>
<p>Table S3 shows PrP and PP-based BKIDC chemical structures.</p>
<p>Figure S15 shows measured and simulated plasma levels in SCID mice.</p>
<p>Figure S8 shows effects of BKIDC-1553 and 1553-N-Me on intracellular ATP levels in LNCaP, PC3, DU145 cells.</p>
<p>Table S7 shows pharmacokinetic parameters after a single dose in mouse, rat, dog, and cynomolgus monkey.</p>
<p>Figure S5 shows cell cycle profiles and histograms of DAPI-stained cells with BKIDCs.</p>
<p>Table S4 shows screening results of BKIDCs tested at 10 µM in LNCaP95 and PC3 cells the MTS assay.</p>
<div>Abstract<p>Metastatic castration-resistant prostate cancer remains incurable regardless of recent therapeutic advances. Prostate tumors display highly glycolytic phenotypes as the progresses. Nonspecific inhibitors glycolysis have not been utilized successfully for chemotherapy, because their penchant to cause systemic toxicity. This study reports preclinical activity, safety, and pharmacokinetics a novel small-molecule candidate, BKIDC-1553, with antiglycolytic activity. We...
<p>Table S5 shows antibodies and corresponding RPPA signal intensities relative to β-actin signals.</p>
<div>Abstract<p>Metastatic castration-resistant prostate cancer remains incurable regardless of recent therapeutic advances. Prostate tumors display highly glycolytic phenotypes as the progresses. Nonspecific inhibitors glycolysis have not been utilized successfully for chemotherapy, because their penchant to cause systemic toxicity. This study reports preclinical activity, safety, and pharmacokinetics a novel small-molecule candidate, BKIDC-1553, with antiglycolytic activity. We...
<p>Table S1 shows BKIDC-1553 intratumoral concentrations after at least 4 weeks of thrice weekly oral dosing 20 mg/kg.</p>
<p>Figure S16 shows metabolites of BKIDC-1553 produced by liver microsomes from rats, dogs, and humans.</p>
<p>Figure S3 shows competitive proteomic profiling of 1553-N-Me.</p>
<p>Figure S1 shows evaluation of Hexokinase activity using LNCaP crude extracts and recombinant enzyme.</p>
<p>Figure S10 shows measurement of the oxygen consumption rate (OCR) and extracellular acidification (ECAR) using Seahorse assay.</p>
<p>Figure S4 shows dose response of multiple cancer cell lines to BKIDC-1553-N-Me and 1817.</p>
<p>Figure S6 shows RPPA Profiling.</p>
<p>Figure S13 shows effects of BKIDCs on intracellular ATP levels in HK1 and HK2 KO LNCaP cell lines.</p>
<p>Figure S2 shows crystal violet-based cell proliferation assay.</p>