A5058170358- Reproductive Biology and Fertility
- Ovarian function and disorders
- Pluripotent Stem Cells Research
- Sperm and Testicular Function
- Renal and related cancers
Vrije Universiteit Brussel
2021-2025
Abstract Biphasic in vitro oocyte maturation (IVM) can be offered as a patient-friendly alternative to conventional ovarian stimulation fertilization (IVF) patients predicted hyper-responsive stimulation. However, cumulative live birth rates after IVM per cycle are lower than for IVF. In different animal species, supplementation of media with oocyte-secreted factors (OSFs) improves developmental competence through the expression pro-ovulatory genes cumulus cells. Whether addition OSFs human...
Abstract In vitro maturation (IVM) is an assisted reproduction technique with reduced hormone-related side-effects. Several attempts to implement IVM in routine practice have failed, primarily due its relatively low efficiency compared conventional fertilization (IVF). Recently, capacitation (CAPA)-IVM—a novel two-step method—has improved the embryology outcomes through synchronizing oocyte nuclear and cytoplasmic maturation. However, gap between CAPA-IVM IVF still noticeable especially...
Establishing an ideal human follicle culture system for oncofertility patients relies mainly on animal models since donor tissue is scarce and often of suboptimal quality. The in vitro developed our laboratory supports the growth prepubertal mouse secondary follicles up to mature oocytes. Given importance glucose preparing oocyte proper maturation, a baseline characterization metabolism both vivo was carried out. Markers glucose-related pathways (glycolysis, tricarboxylic acid [TCA] cycle,...
Abstract Study question Are there significant differences in carbohydrate metabolism trends between vivo and vitro grown mouse antral follicles during oocyte final maturation? Summary answer Glucose characterization GV to MII transition revealed altered metabolic patterns mainly cumulus cells of matured follicles. What is known already For some cancer patients fertility restoration dependent on using efficient follicle culture systems. As human donor ovarian tissue available for research...