A5065867330- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Reproductive Physiology in Livestock
- Ovarian function and disorders
- Genetic and phenotypic traits in livestock
- Veterinary Equine Medical Research
- Reproductive Health and Technologies
- Seed Germination and Physiology
- Assisted Reproductive Technology and Twin Pregnancy
- Business, Innovation, and Economy
- Animal Genetics and Reproduction
- Employment, Labor, and Gender Studies
- Proteins in Food Systems
- Prenatal Screening and Diagnostics
- Renal and related cancers
- Migration, Racism, and Human Rights
- Patient Satisfaction in Healthcare
- Animal health and immunology
- Healthcare Systems and Technology
- Finance, Taxation, and Governance
- Healthcare Policy and Management
- Migration, Ethnicity, and Economy
- Environmental and Cultural Studies in Latin America and Beyond
- Sports and Physical Education Studies
- International Relations in Latin America
Ghent University
2019-2025
Clínica CES
2009-2024
Ghent University Hospital
2023-2024
Division of Undergraduate Education
2021
Male fertility plays a pivotal role in the success rates of vitro embryo production. While livestock breeding programs rigorously select bulls according to their predicted field fertility, specific traits like polyspermy are not routinely evaluated. Despite known negative impact on survival, paternal factors involved remain unclear. In this study, we aimed address gap by evaluating outcomes four bulls, focusing sperm motility, fertilization rates, incidence, development, and quality....
Bovine embryos are typically cultured at reduced oxygen tension to lower the impact of oxidative stress on embryo development. However, oocyte in vitro maturation (IVM) is performed atmospheric since low during has a negative developmental competence. Lycopene, carotenoid, acts as powerful antioxidant and may protect against conditions. Here, we assessed effect adding 0.2 μM lycopene (antioxidant), 5 menadione (pro-oxidant), their combination generation reactive species (ROS) matured oocytes...
Abstract Embryo development is a dynamic process and critical stages may go unnoticed with the use of traditional morphologic assessments, especially timing embryonic divisions aberrant zygotic cleavage patterns. Bovine embryo impaired after oocyte vitrification, but little known about underlying morphokinetic behavior. Here, bovine zygotes from fresh (n = 708) vitrified oocytes 182) were monitored by time-lapse imaging nature early blastomere modeled to find associations blastocyst at day...
Anti-Müllerian hormone (AMH) reflects the population of growing follicles and has been related to mammalian fertility. In horse, clinical application ovum pick-up intracytoplasmic sperm injection (OPU-ICSI) is increasing, but results depend largely on individuality mare. The aim this study was assess AMH as a predictor for OPU-ICSI outcome in horses. Therefore, 103 mares with total follicle count above 10 were included commercial session serum determined using ELISA. Overall, level...
In the last decade, in vitro embryo production horses has become an established clinical practice, but blastocyst rates from vitrified equine oocytes remain low. Cryopreservation impairs oocyte developmental potential, which may be reflected messenger RNA (mRNA) profile. Therefore, this study aimed to compare transcriptome profiles of metaphase II before and after maturation. To do so, three groups were analyzed with sequencing: (1) fresh matured as a control (FR), (2) maturation (VMAT), (3)...
Abstract Whole-genome (WG) abnormalities, such as uniparental diploidy and triploidy, cause fetal death. Occasionally, they coexist with biparental diploid cells in live births. Understanding the origin early development of WG abnormal blastomeres is crucial for explaining formation androgenotes, gynogenotes, chimerism, mixoploidy. By haplotyping 118 from first cleavages, we identified various mechanisms heterogoneic divisions that lead to or their coexistence normal both multipolar bipolar...
Equine oocyte vitrification would benefit the growing in vitro embryo production programs, but further optimization of protocol is necessary to reach clinical efficiency. Therefore, we aimed perform a direct comparison non-permeating and permeating cryoprotective agents (CPAs) during warming equine immature oocytes. In first experiment, cumulus oocytes complexes (COCs) were vitrified comparing sucrose, trehalose, galactose combination with ethylene glycol (EG) dimethyl sulfoxide (DMSO)....
In vivo-matured oocytes exhibit higher developmental competence than those matured in vitro but mimicking the vivo environment by conditions has been challenging. Until now, conventional two-dimensional (2D) systems have used for maturation of bovine cumulus-oocytes-complexes (COCs). However, using such present certain limitations. Therefore, alternative low-cost methodologies may help to optimize oocyte maturation. Here, we two different culture COCs and evaluate their potential influence...
In addition to fulfilling many breeders' curiosity, equine embryonic sex determination can have a profound commercial impact. However, the application of currently described assays for sexing has rendered variable diagnosis and validation rates, with sensitivity being main problem. addition, while pregnancy results in vivo-flushed embryos following needle aspiration biopsy equal those non-biopsied embryos, effect on vitro-produced is unknown. Here, we aimed develop highly sensitive specific...
Cumulus expansion is an important indicator of oocyte maturation and has been suggested to be indicative greater developmental capacity. Although multiple methods have described assess cumulus expansion, none them considered a gold standard. Additionally, these are subjective time-consuming. In this manuscript, the reliability three measurement was assessed, deep learning model created automatically perform measurement. 232 cumulus-oocyte complexes evaluated by independent observers using...
Selenium is commonly used as an antioxidant in a serum-free culture medium setting. However, lycopene has emerged potent being twice efficient β-carotene and 10 times α-tocopherol with beneficial effects when supplemented maturation medium. Here, we aimed to evaluate the effect of supplementation on blastocyst development quality. After vitro fertilization, presumed zygotes were cultured groups 25 50 μl droplets synthetic oviductal fluid. Culture was done using four experimental groups:...
Reproduction, Fertility and Development is an international journal publishing original research , review comment in the fields of reproduction developmental biology humans, domestic animals wildlife
Reproduction, Fertility and Development is an international journal publishing original research , review comment in the fields of reproduction developmental biology humans, domestic animals wildlife