- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Extracellular vesicles in disease
- Radiomics and Machine Learning in Medical Imaging
- Ovarian function and disorders
- Metabolomics and Mass Spectrometry Studies
- Cancer Genomics and Diagnostics
- Reproductive Health and Technologies
- Animal Genetics and Reproduction
- Reproductive System and Pregnancy
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Pluripotent Stem Cells Research
- Bee Products Chemical Analysis
- Effects of Environmental Stressors on Livestock
Ghent University Hospital
2023
Ghent University
2019-2021
Extracellular vesicles (EVs) have been isolated from follicular (FF) and ampullary oviduct fluid (AOF), using different isolation methods. However, it is not clear whether purification methods can affect the functionality of resulting EVs. Here, we compared two (OptiPrep™ density gradient ultracentrifugation (ODG UC) single-step size exclusion chromatography (SEC) (qEV IZON™ single column)) for EVs bovine FF AOF. Additionally, evaluated addition derived either by ODG UC or SEC AOF during...
Excessive production and accumulation of reactive oxygen species (ROS) may cause embryo damage associated with oxidative stress. Lycopene, a natural antioxidant, can scavenge singlet is one the most effective antioxidants among carotenoids. We evaluated effects supplementation lycopene (antioxidant), menadione (prooxidant), their combination during invitro oocyte maturation on ROS generation in matured oocytes quality vitrified-warmed embryos. Cumulus–oocyte complexes, collected from...
Invivo, oocytes mature in the presence of follicular fluid (FF) and ampullary oviductal (AOF), starting from premature to final maturation stages (3-6h postovulation). Extracellular vesicles (EVs) have been identified as important mediators gamete/embryo-maternal interactions. They carry regulatory molecules, such microRNAs mRNAs. So far, functionality EVs derived FF AOF has not yet determined. The aim this study was evaluate effect during invitro oocyte (IVM) on development quality...
Reproduction, Fertility and Development is an international journal publishing original research , review comment in the fields of reproduction developmental biology humans, domestic animals wildlife