A5021348757- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Ovarian function and disorders
- Reproductive Health and Technologies
- Genetic and phenotypic traits in livestock
- MicroRNA in disease regulation
- Assisted Reproductive Technology and Twin Pregnancy
- Reproductive System and Pregnancy
- Extracellular vesicles in disease
- Seed Germination and Physiology
- Bee Products Chemical Analysis
- Renal and related cancers
- Animal Genetics and Reproduction
- Pluripotent Stem Cells Research
- Reproductive Physiology in Livestock
- Pickering emulsions and particle stabilization
- Bone Metabolism and Diseases
- RNA Interference and Gene Delivery
- Effects of Environmental Stressors on Livestock
Ghent University
2020-2024
University of Tennessee at Knoxville
2024
Ghent University Hospital
2023
University of Antwerp
2022
Royan Institute
2016
Academic Center for Education, Culture and Research
2016
Isfahan University of Technology
2016
Extracellular vesicles (EVs) have been isolated from follicular (FF) and ampullary oviduct fluid (AOF), using different isolation methods. However, it is not clear whether purification methods can affect the functionality of resulting EVs. Here, we compared two (OptiPrep™ density gradient ultracentrifugation (ODG UC) single-step size exclusion chromatography (SEC) (qEV IZON™ single column)) for EVs bovine FF AOF. Additionally, evaluated addition derived either by ODG UC or SEC AOF during...
Abstract Background Within the follicular fluid, extracellular vesicles (EVs) guide oocyte growth through their cargo microRNAs (miRNAs). Here, we investigated role of EVs and miRNAs by linking found in EVs, derived from fluid an individual follicle, to ability its become a blastocyst (competent) or not (non-competent). Methods Bovine antral follicles were dissected, categorized as small (2–4 mm) large (5–8 corresponding oocytes subjected maturation, fertilization embryo culture stage....
Bovine embryos are typically cultured at reduced oxygen tension to lower the impact of oxidative stress on embryo development. However, oocyte in vitro maturation (IVM) is performed atmospheric since low during has a negative developmental competence. Lycopene, carotenoid, acts as powerful antioxidant and may protect against conditions. Here, we assessed effect adding 0.2 μM lycopene (antioxidant), 5 menadione (pro-oxidant), their combination generation reactive species (ROS) matured oocytes...
Abstract Embryo development is a dynamic process and critical stages may go unnoticed with the use of traditional morphologic assessments, especially timing embryonic divisions aberrant zygotic cleavage patterns. Bovine embryo impaired after oocyte vitrification, but little known about underlying morphokinetic behavior. Here, bovine zygotes from fresh (n = 708) vitrified oocytes 182) were monitored by time-lapse imaging nature early blastomere modeled to find associations blastocyst at day...
Abstract Improved oocyte competence for embryo development and pregnancy was observed following ovulation of preovulatory follicles with greater physiological maturity, as indicated by estradiol production, prior to the gonadotropin-releasing hormone (GnRH)-induced luteinizing (LH) surge. It hypothesized that follicular fluid from maturity better supports maturing oocyte's metabolic requirements improves development. The objective determine if differences in due follicle influence metabolism...
Extracellular vesicles (EVs) contain microRNAs (miRNAs), which are important regulators of embryonic development. Nevertheless, little is known about the precise molecular processes controlling blastocyst development and quality. In a previous study, we identified bta-miR-665 as one miRNAs more abundantly present in extracellular embryo-conditioned culture media blastocysts compared to degenerate ones. Here, investigated effect regulatory roles by supplementation mimics or inhibitors media....
Equine oocyte vitrification would benefit the growing in vitro embryo production programs, but further optimization of protocol is necessary to reach clinical efficiency. Therefore, we aimed perform a direct comparison non-permeating and permeating cryoprotective agents (CPAs) during warming equine immature oocytes. In first experiment, cumulus oocytes complexes (COCs) were vitrified comparing sucrose, trehalose, galactose combination with ethylene glycol (EG) dimethyl sulfoxide (DMSO)....
In vivo-matured oocytes exhibit higher developmental competence than those matured in vitro but mimicking the vivo environment by conditions has been challenging. Until now, conventional two-dimensional (2D) systems have used for maturation of bovine cumulus-oocytes-complexes (COCs). However, using such present certain limitations. Therefore, alternative low-cost methodologies may help to optimize oocyte maturation. Here, we two different culture COCs and evaluate their potential influence...
Cumulus expansion is an important indicator of oocyte maturation and has been suggested to be indicative greater developmental capacity. Although multiple methods have described assess cumulus expansion, none them considered a gold standard. Additionally, these are subjective time-consuming. In this manuscript, the reliability three measurement was assessed, deep learning model created automatically perform measurement. 232 cumulus-oocyte complexes evaluated by independent observers using...
Selenium is commonly used as an antioxidant in a serum-free culture medium setting. However, lycopene has emerged potent being twice efficient β-carotene and 10 times α-tocopherol with beneficial effects when supplemented maturation medium. Here, we aimed to evaluate the effect of supplementation on blastocyst development quality. After vitro fertilization, presumed zygotes were cultured groups 25 50 μl droplets synthetic oviductal fluid. Culture was done using four experimental groups:...
Abstract Cumulus expansion is an important indicator of oocyte maturation, often correlated with greater developmental capacity. Although multiple methods have been described to assess cumulus expansion, none them considered a gold standard. Additionally, these are subjective and time-consuming. Here, the reliability three measurement was evaluated deep learning model created automatically perform measurement. Cumulus-oocyte complexes were compared before after in vitro maturation by...
Excessive production and accumulation of reactive oxygen species (ROS) may cause embryo damage associated with oxidative stress. Lycopene, a natural antioxidant, can scavenge singlet is one the most effective antioxidants among carotenoids. We evaluated effects supplementation lycopene (antioxidant), menadione (prooxidant), their combination during invitro oocyte maturation on ROS generation in matured oocytes quality vitrified-warmed embryos. Cumulus–oocyte complexes, collected from...
Reproduction, Fertility and Development is an international journal publishing original research , review comment in the fields of reproduction developmental biology humans, domestic animals wildlife
Abstract In vivo matured oocytes exhibit higher developmental competence than those in vitro , but mimicking the environment by conditions has been challenging. Till now, conventional two-dimensional (2D) systems have used for maturation of bovine cumulus-oocytes-complexes (COCs). However, using such may cause cell flattening and does not allow cumulus expansion all dimensions, which is less physiological. Therefore, implementing a low-cost highly effective -like microenvironment methodology...
Reproduction, Fertility and Development is an international journal publishing original research , review comment in the fields of reproduction developmental biology humans, domestic animals wildlife