A5016477520- Reproductive Biology and Fertility
- Sperm and Testicular Function
- MicroRNA in disease regulation
- Ovarian function and disorders
- Reproductive Health and Technologies
- Seed Germination and Physiology
- Extracellular vesicles in disease
- Reproductive System and Pregnancy
- Genetic and phenotypic traits in livestock
- Assisted Reproductive Technology and Twin Pregnancy
- 3D Printing in Biomedical Research
- Pickering emulsions and particle stabilization
- Bee Products Chemical Analysis
- Reproductive Physiology in Livestock
- Effects of Environmental Stressors on Livestock
- Cancer-related molecular mechanisms research
- Tissue Engineering and Regenerative Medicine
- Renal and related cancers
- Pluripotent Stem Cells Research
- Nanoplatforms for cancer theranostics
Ghent University
2021-2024
Inserm
2024
Université de Montpellier
2024
Centre National de la Recherche Scientifique
2024
Abstract Background Within the follicular fluid, extracellular vesicles (EVs) guide oocyte growth through their cargo microRNAs (miRNAs). Here, we investigated role of EVs and miRNAs by linking found in EVs, derived from fluid an individual follicle, to ability its become a blastocyst (competent) or not (non-competent). Methods Bovine antral follicles were dissected, categorized as small (2–4 mm) large (5–8 corresponding oocytes subjected maturation, fertilization embryo culture stage....
Abstract Embryo development is a dynamic process and critical stages may go unnoticed with the use of traditional morphologic assessments, especially timing embryonic divisions aberrant zygotic cleavage patterns. Bovine embryo impaired after oocyte vitrification, but little known about underlying morphokinetic behavior. Here, bovine zygotes from fresh (n = 708) vitrified oocytes 182) were monitored by time-lapse imaging nature early blastomere modeled to find associations blastocyst at day...
Extracellular vesicles (EVs) contain microRNAs (miRNAs), which are important regulators of embryonic development. Nevertheless, little is known about the precise molecular processes controlling blastocyst development and quality. In a previous study, we identified bta-miR-665 as one miRNAs more abundantly present in extracellular embryo-conditioned culture media blastocysts compared to degenerate ones. Here, investigated effect regulatory roles by supplementation mimics or inhibitors media....
Equine oocyte vitrification would benefit the growing in vitro embryo production programs, but further optimization of protocol is necessary to reach clinical efficiency. Therefore, we aimed perform a direct comparison non-permeating and permeating cryoprotective agents (CPAs) during warming equine immature oocytes. In first experiment, cumulus oocytes complexes (COCs) were vitrified comparing sucrose, trehalose, galactose combination with ethylene glycol (EG) dimethyl sulfoxide (DMSO)....
In vivo-matured oocytes exhibit higher developmental competence than those matured in vitro but mimicking the vivo environment by conditions has been challenging. Until now, conventional two-dimensional (2D) systems have used for maturation of bovine cumulus-oocytes-complexes (COCs). However, using such present certain limitations. Therefore, alternative low-cost methodologies may help to optimize oocyte maturation. Here, we two different culture COCs and evaluate their potential influence...
Selenium is commonly used as an antioxidant in a serum-free culture medium setting. However, lycopene has emerged potent being twice efficient β-carotene and 10 times α-tocopherol with beneficial effects when supplemented maturation medium. Here, we aimed to evaluate the effect of supplementation on blastocyst development quality. After vitro fertilization, presumed zygotes were cultured groups 25 50 μl droplets synthetic oviductal fluid. Culture was done using four experimental groups:...
Reproduction, Fertility and Development is an international journal publishing original research , review comment in the fields of reproduction developmental biology humans, domestic animals wildlife
Abstract In vivo matured oocytes exhibit higher developmental competence than those in vitro , but mimicking the environment by conditions has been challenging. Till now, conventional two-dimensional (2D) systems have used for maturation of bovine cumulus-oocytes-complexes (COCs). However, using such may cause cell flattening and does not allow cumulus expansion all dimensions, which is less physiological. Therefore, implementing a low-cost highly effective -like microenvironment methodology...
Reproduction, Fertility and Development is an international journal publishing original research , review comment in the fields of reproduction developmental biology humans, domestic animals wildlife
Reproduction, Fertility and Development is an international journal publishing original research , review comment in the fields of reproduction developmental biology humans, domestic animals wildlife
Reproduction, Fertility and Development is an international journal publishing original research , review comment in the fields of reproduction developmental biology humans, domestic animals wildlife
Reproduction, Fertility and Development is an international journal publishing original research , review comment in the fields of reproduction developmental biology humans, domestic animals wildlife
Reproduction, Fertility and Development is an international journal publishing original research , review comment in the fields of reproduction developmental biology humans, domestic animals wildlife