A5036275832- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Prenatal Screening and Diagnostics
- Reproductive Physiology in Livestock
- Genetic Syndromes and Imprinting
- Genetic and phenotypic traits in livestock
- Chromosomal and Genetic Variations
- Genomic variations and chromosomal abnormalities
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Animal Genetics and Reproduction
- Parasitic infections in humans and animals
- Bioenergy crop production and management
- Renal and related cancers
- Assisted Reproductive Technology and Twin Pregnancy
- Amoebic Infections and Treatments
- Cleft Lip and Palate Research
- Genetic Mapping and Diversity in Plants and Animals
- Reproductive Health and Technologies
- Pluripotent Stem Cells Research
- Parasite Biology and Host Interactions
- Parathyroid Disorders and Treatments
- Craniofacial Disorders and Treatments
- Parasites and Host Interactions
- Seed Germination and Physiology
- Ovarian function and disorders
Ghent University
2018-2025
Ghent University Hospital
2024
KU Leuven
2021-2024
Cordoba University
2023
Abstract In different species, embryonic aneuploidies and genome-wide errors are a major cause of developmental failure. The increasing number equine embryos being produced worldwide provides the opportunity to characterize rank or select based on their genetic profile prior transfer. Here, we explored possibility generic, preimplantation testing concurrently for (PGT-A) monogenic (PGT-M) traits diseases in horse, meanwhile assessing incidence spectrum chromosomal vitro - embryos. To this...
Male fertility plays a pivotal role in the success rates of vitro embryo production. While livestock breeding programs rigorously select bulls according to their predicted field fertility, specific traits like polyspermy are not routinely evaluated. Despite known negative impact on survival, paternal factors involved remain unclear. In this study, we aimed address gap by evaluating outcomes four bulls, focusing sperm motility, fertilization rates, incidence, development, and quality....
During normal zygotic division, two haploid parental genomes replicate, unite and segregate into biparental diploid blastomeres.
Abstract Embryo development is a dynamic process and critical stages may go unnoticed with the use of traditional morphologic assessments, especially timing embryonic divisions aberrant zygotic cleavage patterns. Bovine embryo impaired after oocyte vitrification, but little known about underlying morphokinetic behavior. Here, bovine zygotes from fresh (n = 708) vitrified oocytes 182) were monitored by time-lapse imaging nature early blastomere modeled to find associations blastocyst at day...
Anti-Müllerian hormone (AMH) reflects the population of growing follicles and has been related to mammalian fertility. In horse, clinical application ovum pick-up intracytoplasmic sperm injection (OPU-ICSI) is increasing, but results depend largely on individuality mare. The aim this study was assess AMH as a predictor for OPU-ICSI outcome in horses. Therefore, 103 mares with total follicle count above 10 were included commercial session serum determined using ELISA. Overall, level...
In the last decade, in vitro embryo production horses has become an established clinical practice, but blastocyst rates from vitrified equine oocytes remain low. Cryopreservation impairs oocyte developmental potential, which may be reflected messenger RNA (mRNA) profile. Therefore, this study aimed to compare transcriptome profiles of metaphase II before and after maturation. To do so, three groups were analyzed with sequencing: (1) fresh matured as a control (FR), (2) maturation (VMAT), (3)...
Abstract Whole-genome (WG) abnormalities, such as uniparental diploidy and triploidy, cause fetal death. Occasionally, they coexist with biparental diploid cells in live births. Understanding the origin early development of WG abnormal blastomeres is crucial for explaining formation androgenotes, gynogenotes, chimerism, mixoploidy. By haplotyping 118 from first cleavages, we identified various mechanisms heterogoneic divisions that lead to or their coexistence normal both multipolar bipolar...
Equine oocyte vitrification would benefit the growing in vitro embryo production programs, but further optimization of protocol is necessary to reach clinical efficiency. Therefore, we aimed perform a direct comparison non-permeating and permeating cryoprotective agents (CPAs) during warming equine immature oocytes. In first experiment, cumulus oocytes complexes (COCs) were vitrified comparing sucrose, trehalose, galactose combination with ethylene glycol (EG) dimethyl sulfoxide (DMSO)....
In addition to fulfilling many breeders' curiosity, equine embryonic sex determination can have a profound commercial impact. However, the application of currently described assays for sexing has rendered variable diagnosis and validation rates, with sensitivity being main problem. addition, while pregnancy results in vivo-flushed embryos following needle aspiration biopsy equal those non-biopsied embryos, effect on vitro-produced is unknown. Here, we aimed develop highly sensitive specific...
Recently mutations in single genes have been found to cause dentofacial deviations like hypodontia, cleft lip and palate, as well some craniofacial malformation syndromes Aperts/Crouzon syndromes, cleidocranial dysostosis... These findings may lead an overestimation oversimplification of the role our daily life. The "one gene, one disease" principle has applied too often, while most complex human characteristics are determined by a large number genes, each leading increased liability for...
Abstract In different species, embryonic chromosomal aneuploidies are a major cause of developmental failure. The increasing number equine embryos being produced worldwide provides the opportunity to characterize and rank or select based on their genetic profile prior transfer. Here, we explore possibility generic, genome-wide preimplantation testing concurrently for (PGT-A), monogenic (PGT-M) polygenic (PGT-P) traits diseases in horse. To this end, over 70,000 single nucleotide polymorphism...
Abstract The zygotic division enables two haploid genomes to segregate into biparental diploid blastomeres. This fundamental tenet was challenged by the observation that blastomeres with different genome ploidy or parental genotypes can coexist within individual embryos. We hypothesized whole distinct blastomere lineages during first through “heterogoneic division”. Here, we map genomic landscape of 82 from 25 embryos underwent multipolar division. coexistence androgenetic and polyploid...
Reproduction, Fertility and Development is an international journal publishing original research , review comment in the fields of reproduction developmental biology humans, domestic animals wildlife
The mammalian zygotic cleavage is expected to result in two mononuclear blastomeres. However, zygotes undergoing multipolar divisions resulting direct into three or four cells are frequently observed bovine and human embryonic development have been associated with decreased euploidy rates of blastocysts a lower pregnancy rate (Somfai et al. 2010 J. Reprod. Dev. 56, 200-207; https://doi.org/10.1262/jrd.09-097a; Zhan 2016 PLoS ONE 11, 1-19; https://doi.org/10.1371/journal.pone.0166398;...