A5064793113- Corneal surgery and disorders
- Corneal Surgery and Treatments
- Retinal Diseases and Treatments
- Retinal and Macular Surgery
- Intraocular Surgery and Lenses
- Ocular Surface and Contact Lens
- Retinal and Optic Conditions
- Retinal Development and Disorders
- Colorectal and Anal Carcinomas
- Glaucoma and retinal disorders
- Photoreceptor and optogenetics research
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Retinal Imaging and Analysis
- Advanced Glycation End Products research
- RNA Research and Splicing
- Ocular Oncology and Treatments
- Diverticular Disease and Complications
- RNA regulation and disease
- Cystic Fibrosis Research Advances
- Ophthalmology and Visual Health Research
- Intraperitoneal and Appendiceal Malignancies
- Psoriasis: Treatment and Pathogenesis
- Neurological Complications and Syndromes
- Families in Therapy and Culture
- Islamic Thought and Society Studies
The Francis Crick Institute
2024
EES Research (United Kingdom)
2024
Newcastle University
2018-2022
Sunderland Eye Infirmary
2020
South Tyneside and Sunderland NHS Foundation Trust
2020
Centre for Life
2018-2019
James Cook University Hospital
2018-2019
Royal Victoria Infirmary
2019
University of Newcastle Australia
2019
Kırıkkale University
2017
Cell replacement therapy is a promising treatment for irreversible retinal cell death in diverse diseases such as Stargardt's disease, age-related macular degeneration, and retinitis pigmentosa. The final impact of all dystrophies the loss photoreceptors; hence, there pressing need research into replacement. Seminal work has shown that simple three-dimensional culture system enables differentiation human pluripotent stem cells to organoids containing large numbers photoreceptors developing...
Abstract Induced pluripotent stem cell (iPSC)-derived retinal organoids provide a platform to study human retinogenesis, disease modeling, and compound screening. Although may represent tissue structures with greater physiological relevance the in vivo retina, their generation is not without limitations. Various protocols have been developed enable development of all major types; however, variability across iPSC lines often reported. Modulating signaling pathways important for eye formation,...
RNA editing is a posttranscriptional mechanism that targets changes in transcripts to modulate innate immune responses. We report the role of astrocyte-specific, ADAR1-mediated neuroinflammation Parkinson’s disease (PD). generated human induced pluripotent stem cell–derived astrocytes, neurons and cocultures exposed them small soluble alpha-synuclein aggregates. Oligomeric triggered an inflammatory glial state associated with Toll-like receptor activation, viral responses, cytokine...
Abstract Nerve growth factor (NGF) has demonstrated great benefit in the treatment of neurotrophic corneal ulcers. There is evidence for multiple modes action promoting healing, but only indirect exists NGF's effects on limbal stem cells (LSCs). Understanding role NGF LSC biology will improve our understanding paracrine regulation niche and design cell-based therapies conditions such as deficiency. In this article, we studied signaling components during differentiation proliferation...
Abstract One of the main challenges in limbal stem cell (LSC) biology and transplantation is lack definitive surface markers which can be used to identify enrich viable LSCs. In this study, expression 361 proteins was assessed ex vivo expanded epithelial cells. marker, CD200 selected for further characterization based on a small subset cells (2.25% ± 0.69%) reduced through consecutive passaging calcium induced differentiation. localized population at basal layer human mouse epithelium....
Limbal stem cell (LSC) deficiency causes progressive loss of vision but may be treated by transplant autologous LSCs. Cryopreservation has the potential to indefinitely extend lifespan LSCs allowing re-transplant in case graft failure. In this study, we aimed identify optimal cryoprotectant and concentration for LSC cultures. Suspension cultures derived from cadaveric corneoscleral rims were cooled 4 °C with Me2SO, propylene glycol or ethylene at a 5%, 10% 15%. Cell tolerance was measured...
Abstract RNA editing is a post transcriptional mechanism that targets changes in transcripts to modulate innate immune responses. We report the role of astrocyte specific, ADAR1 mediated neuroinflammation Parkinson’s disease. generated hiPSC-derived astrocytes, neurons and co-cultures exposed them small soluble alpha-synuclein aggregates. Oligomeric triggered an inflammatory glial state associated with TLR activation, viral responses, cytokine secretion. This reactive resulted loss...
The definitive treatment of anal cancer with chemoradiotherapy spares abdominoperineal resection for salvage but carries a high burden toxicity. Intensity-modulated radiation therapy has been implemented to reduce toxicity, breaks and improve survival. However, large long-term studies are lacking. We aimed investigate the toxicities survival patients treated intensity-modulated at James Cook University Hospital, Middlesbrough.We conducted retrospective analysis all squamous cell Hospital...
Abstract Objectives To validate and update the 2013 James Lind Alliance (JLA) Sight Loss Vision Priority Setting Partnership (PSP)’s research priorities for Ophthalmology, as part of UK Clinical Eye Research Strategy. Methods Twelve ophthalmology themes were identified from JLA report. They allocated to five Study Groups diverse stakeholders who reviewed top 10 each theme. Using an online survey (April 2021-February 2023), respondents invited complete one or more nine subspecialty surveys....
Despite major advances in spatial RNA sequencing, the ability to extract temporal information sequencing experiments is still limited. Here, we describe Transcriptome Timestamping (T2), a system which harnesses naturally occurring A-to-I editing of transcripts unmodified human cells infer transcriptional history. T2 provides age estimates for individual transcripts, and serves as an endogenous molecular recorder, differentiating between complex programs. We show that can identify transient...
Endothelial cell density (ECD) changes long after penetrating keratoplasty (PKP) of organ-cultured corneas have been little studied. We aim to calculate the point when ECD decline stabilises following PKP with organ culture stored corneas.This is an observational study first-ever PKPs and re-grafts, performed over 17 years under a single surgeon. ECDs were acquired at 3 6 months, 1 year post-graft annually thereafter by specular microscopy. Time-dependent data was fitted log-biexponential...
Squamous cell carcinoma is the most common variant of anal malignancy. Certain disease-related factors have been established in determining survival. These include tumour size, differentiation and nodal involvement. Other such as HIV status, human papillomavirus infection, smoking socioeconomic disparity may important roles, however few data are available on UK population. We aim to correlate social deprivation survival cancer patients at a tertiary centre.All consecutive cases diagnosed...
<h3>Introduction and objectives</h3> SM is predominantly associated with hospital-acquired sepsis. It occurs naturally in soil water has a propensity for antimicrobial resistance. Its role adult bronchiectasis, risk factors colonisation pathogenicity unknown. We sought to identify characteristics the isolation of this pathogen sputum samples, antibiotic resistance clinical outcomes. <h3>Methods</h3> A longitudinal, retrospective analysis was conducted specialist bronchiectasis unit North...
<h3>Introduction and objectives</h3> Prompt diagnosis, risk stratification, treatment follow-up are essential for a favourable outcome in the management of PE. We examined number important factors significant PE from hospital admission to end period six months. <h3>Methods</h3> A retrospective cross-sectional study was performed large tertiary North East England following review CT Pulmonary Angiography (CTPA) reports over 12 month period. Inclusion criteria included: high pre-test...