A5050753376- Ion channel regulation and function
- Neurobiology and Insect Physiology Research
- Sperm and Testicular Function
- Electrochemical Analysis and Applications
- Force Microscopy Techniques and Applications
- Neuroendocrine regulation and behavior
- Neuroscience and Neural Engineering
- Reproductive Biology and Fertility
- Head and Neck Cancer Studies
- Analytical Chemistry and Sensors
- Reproductive biology and impacts on aquatic species
- Photoreceptor and optogenetics research
- Cardiac electrophysiology and arrhythmias
- Ion Transport and Channel Regulation
- Dysphagia Assessment and Management
- Ion Channels and Receptors
- Pain Mechanisms and Treatments
- Electrolyte and hormonal disorders
- Voice and Speech Disorders
- Marine Biology and Environmental Chemistry
- Neuroscience and Neuropharmacology Research
- Circadian rhythm and melatonin
- Apelin-related biomedical research
- melanin and skin pigmentation
- Cancer survivorship and care
Centro de Investigación en Materiales Avanzados
2011-2022
Center for Research and Advanced Studies of the National Polytechnic Institute
2007-2019
Instituto Politécnico Nacional
2009-2019
Universidad de Antioquia
2016-2018
London Centre for Nanotechnology
2006-2009
Imperial College London
2004-2009
Nanomed (Brazil)
2008
Universidad Nacional Autónoma de México
1996-2007
Hammersmith Hospital
2006
Medical Research Council
2005
Do not touch! The surface of a living cell is soft and responsive therefore high-resolution imaging the membrane has been possible to date. Now noncontact protein complexes in plasma cells demonstrated (see picture) used follow cells' structural reorganization. This breakthrough opens up wealth new experiments biology. Supporting information for this article available on WWW under http://www.wiley-vch.de/contents/jc_2002/2006/z503915_s.pdf or from author. Please note: publisher responsible...
Cannabinoid receptor 2 (CB2) agonists provide the potential for treating chronic pain states without CNS effects associated with CB1 activation. Animal models suggest that they act mainly via non-neuronal cells, possibly inhibition of inflammatory cells in periphery or CNS, release beta-endorphin; however, clinical relevance and mechanism analgesic action is uncertain. Here, we demonstrate colocalisation CB2 capsaicin TRPV1 human dorsal root ganglion (DRG) sensory neurons increased levels...
Aldosterone, the most important sodium-retaining hormone, was first characterized >50 years ago. However, despite numerous studies including classical work of Isidore S. “Izzy” Edelman showing that aldosterone action depended on ATP production, mechanism by which it activates sodium reabsorption via epithelial channel remains unclear. Here, we report experiments suggest one key steps in is an autocrine/paracrine system. The hormone stimulates release from basolateral side target kidney...
Extracellular ATP initiates potent effects on sodium transport across renal epithelia through membrane-associated purinergic receptors. Dependent the location of these receptors, either inhibits or stimulates reabsorption. Using A6 cells, transepithelial electrical resistance measurements, and scanning ion conductance microscopy, we have identified receptors involved in stimulatory action epithelial cell basolateral plasma membrane. Addition P2X 4 receptor agonist 2-methylthio-ATP (2MeSATP)...
Renal proximal tubular sodium and glucose reabsorption are regulated by the sodium-glucose cotransporter (SGLT2). Changes in this transporter can play a role hyperglycaemia reactive oxygen species (ROS) production. We demonstrated increased absorption tubule membrane vesicles expression of SGLT2 hypertensive rats. Here we investigated Angiotensin II (Ang II) -dependent induction development Ang II-dependent kidney damage. The aim study was to determine whether is associated with propose...
Berühren verboten! Die Oberfläche lebender Zellen ist weich und nachgiebig, weshalb bisher eine hochauflösende Abbildung der Zellmembran nicht möglich war. Nun gelang das kontaktlose Abbilden von Proteinkomplexen in Plasmamembran (siehe Bild) Verfolgen strukturellen Reorganisation Zellen. Dieser Durchbruch eröffnet den Zugang zu einer Vielzahl neuer Experimente Membran- Zellbiologie. Supporting information for this article is available on the WWW under...
Stem cells, that is, cells can both reproduce themselves and differentiate into functional cell types, attract much interest as potential aids to healing disease therapy. Embryonic neural crest is pluripotent generates the peripheral nervous system, melanocytes, some connective tissues. Neural-crest-related stem have been reported previously in postnatal skin: committed melanocytic hair follicle, types from follicle papilla produce various sets of lineages. Here we describe novel crest-like...
Summary Cyclic guanosine monophosphate ( cGMP ) has been recently shown to modulate in vitro capacitation of mammalian spermatozoa, but the mechanisms through which it influences sperm functions have not clarified. There are at least two targets , cyclic nucleotide‐gated (CNG) channels and ‐dependent protein kinase (PKG), involved several physiological events spermatozoa. It suggested that CNG allow influx Ca 2+ cytoplasm during capacitation, whereas PKG could trigger a phosphorylation...
Components of the sea urchin outer egg jelly layer such as speract drastically change second messenger levels and membrane permeability in sperm. Ion channels are deeply involved sperm-egg dialogue other species. Yet, due to small size sperm, studies ion their modulation by messengers sperm scarce. In this report we offer first direct evidence that cation-selective upwardly regulated cAMP operate Due poor selectivity among monovalent cations, channel activation seawater could contribute...
During capacitation of mammalian sperm intracellular [Ca(2+)] and cyclic nucleotides increase, suggesting that CNG channels play a role in the physiology sperm. Here we study effect capacitation, 8Br-cAMP (8-bromoadenosine 3',5'-cyclic monophosphate) 8Br-cGMP (8-bromoguanosine on macroscopic ionic currents mouse sperm, finding existence different populations terms recorded current its response to nucleotides. Our results show increase current, having differential sensitivity cGMP (cyclic...
The study of motor properties cells under appropriate physical-chemical conditions is a significant problem nowadays. standard techniques presently used do not allow to evaluate neither large samples nor control their thermodynamic conditions. In this work, we report cell motility sensor based on an optical technique with time-resolved correlation, adapted in system able several simultaneously. Image correlation analysis follow temporal behavior. A wide variety motile cells, such as archaea,...
Abstract Ion permeability changes involving ion channels and transporters are essential for fertilization, since they key elements in sperm‐egg signaling environmental sensing. Changes modulated by external factors components from the outer layer of homologous egg, like sperm‐activating peptides fucose‐sulfate glycoconjugate, trigger complex systems sperm that modulate how swim, find egg fertilize it. These depend on plasma membrane involve an alteration second messenger levels, potential...