Background Nitrite is a nitric oxide (NO) metabolite in tissues and blood, which can be converted to NO under hypoxia facilitate tissue perfusion. Although nitrite known cause vasodilation following its reduction NO, the effect of on platelet activity remains unclear. In this study, nitrite+erythrocytes, with without deoxygenation, was investigated. Methodology/Finding Platelet aggregation studied platelet-rich plasma (PRP) PRP+erythrocytes by turbidimetric impedance aggregometry,...

Summary Thromboembolic complications have been documented in thalassaemia patients. The aggregability of abnormal red blood cells and the high level membrane‐derived microparticles (MPs) stemming from are thought to be responsible for associated thrombotic risk. We investigated number MPs, their cellular origin procoagulant properties β ‐thalassaemia. Fresh whole was simultaneously stained annexin V, antigens known density beads. these phosphatidylserine (PS)‐bearing MPs were also measured...

Iron chelators have been reported to induce apoptosis and cell cycle arrest in cancer cells. Recent studies suggest broad selective antitumor activity of the new iron chelator, di-2-pyridylketone-4,4-dimethyl-3-thiosemicarbazone (Dp44mT; Whitnall et al., Proc Natl Acad Sci USA 2006;103:14901-14906). However, little is known concerning its effects on hematological malignancies. Using acute leukemia cells, effect Dp44mT apoptosis, cycle, caspase-3 activation, mitochondrial trans-membrane...

Abstract BACKGROUND Thalassemia is a hereditary hemolytic anemia caused by mutations in the globin gene complex. Circulatory disturbances including arterial and venous thrombosis have also been noted these patients. Aggregability of abnormal RBC high level membrane‐derived microparticles stemming from activated platelets other blood cells are thought to be responsible for associated thrombotic risk. Destruction an important pathophysiological consequence, particularly through formation...

Iron deficiency affects 500 million people, yet the molecular role of iron in gene expression remains poorly characterized. In addition, alterations global after chelation remain unclear and are important to assess for understanding pathology biological effects chelators. Considering this, we assessed effect on whole genome two chelators (desferrioxamine 2-hydroxy-1-napthylaldehyde isonicotinoyl hydrazone) that have markedly different permeability properties. Sixteen genes were significantly...

Abstract Background The current method of CD4 enumeration in Thailand, based on the three‐tube, three‐color recommended by Centers for Disease Control and Prevention, is expensive thus unavailable to most patients who have human immunodeficiency virus (HIV). Less expensive, simpler protocols (i.e., Pan Leuco gating primary gating) been described but require more published validation data gain widespread acceptance. We describe a multicenter evaluation method. Methods using generic reagents...

Various assays are used to enumerate peripheral blood absolute CD4+ T-lymphocytes. Flow cytometry is considered the gold standard for this purpose. However, high cost of available flow cytometers and monoclonal antibody reagents make it difficult implement such methods in resource-poor settings. In study, we evaluated a cheaper, recently developed single-platform microcapillary cytometer T-lymphocyte enumeration, personal cell analyzer (PCA), from Guava Technologies.CD4+ CD8+ counts whole...

ABSTRACT Use of the standard dual-platform flow cytometric method for determination CD4 + T-lymphocyte counts, which needs both a cytometer (FCM) and hematological analyzer, would inevitably lead to increased variability. The development new single-platform (SP) FCMs that provide direct counts improved assay precision accuracy have recently attracted attention. This study evaluated one those systems, CyFlow green (Partec), single-parameter SP volumetric FCM. performance was compared with two...

Infection is one of the most common causes death in β-thalassemia patients. This may be due part to an underlying immunological abnormality. During past decade, a subset CD3+ T cells that express both CD4+CD8+ (DP) T-cells were discovered and have been described several pathological conditions. However, phenotypic characterization this unique T-lymphocyte patients with has not yet investigated.Flow cytometry was used determine frequency such CD4+CD8+(DP) concert frequencies CD4+, CD8+, NKT...

Abstract Background: The standard three‐tube, three‐color flow cytometric method utilizing the TriTEST™ reagents in conjunction with MultiSET™ software commonly used most laboratories Thailand for CD4 enumeration is expensive and thus unavailable to HIV‐infected patients. A more affordable method, i.e., Pan Leuco gating protocol using only two monoclonal antibody reagents, has been described but requires use of CellQUEST™ that does not have automatic reporting facilities. We describe a...

CD4(+) T-lymphocyte count is an important marker in management of HIV-1-infected patients. The standard single-platform (SP) flow cytometric (FCM) testing that uses the known reference microbeads expensive; more affordable alternatives are therefore needed. We evaluated use glutaraldehyde-fixed chicken red blood cells (CRBCs) as counting beads alternative for enumerating counts 87 Linear regression analyses revealed excellent correlation SP FCM using CRBCs with bead-based method...

To determine the number and intensity of phosphatidylserine (PS) expression red blood cells (RBCs), fragmented RBCs, RBC-derived microparticles (RMPs) in patients with β-thalassemia/hemoglobin (Hb)E.We used flow cytometry to levels PS expression.The PS-exposing RBCs was statistically significantly higher (P <.001) than that or RMPs. In contrast, RMPs RBCs. Our study showed a trend association between RBC distribution width (RDW) both their expression.In β-thalassemia/HbE, all differed...

Abstract Background: We have previously developed an alternative approach for undertaking absolute cell counting based upon flow‐rate calibration using bead (FCB), in which (CB) can be used as a material generating the microparticle counts. Here, we extended our work of CD4+ T‐lymphocytes HIV‐infected blood samples with FCB method. Methods: T‐lymphocyte counts EDTA from 30 healthy subjects and 80 HIV‐1‐infected patients were determined TriTEST reagent. The measured by FCB, results compared...

Heinz bodies are important to diagnosing and managing patients. However, microscopic examination of has several disadvantages, demonstrating the need for a better method. We explored potential use flow cytometry examine bodies.Whole-blood samples were collected from patients deficient in G6PD healthy volunteers. Acetylphenylhydrazine was used induce formation red blood cells (RBCs). Then, RBCs positive examined using FACSCanto II cytometer.RBCs treated with acetylphenylhydrazine formed...