The appropriate COVID-19 booster vaccine following inactivated or adenoviral vector vaccination is unclear.

Inactivated vaccine (CoronaVac) and chimpanzee adenovirus-vector (ChAdOx1) have been widely used in resource-limited settings. However, the information on reactogenicity immunogenicity of these two vaccines same setting are limited.Healthy health care workers (HCWs) aged 18 years or older were randomly assigned to receive either doses CoronaVac at 4 weeks interval ChAdOx1 10 interval. Self-reported adverse events (AEs) collected for 7 days following each vaccination. Immunogenicity was...

Summary Thromboembolic complications have been documented in thalassaemia patients. The aggregability of abnormal red blood cells and the high level membrane‐derived microparticles (MPs) stemming from are thought to be responsible for associated thrombotic risk. We investigated number MPs, their cellular origin procoagulant properties β ‐thalassaemia. Fresh whole was simultaneously stained annexin V, antigens known density beads. these phosphatidylserine (PS)‐bearing MPs were also measured...

Enumeration of CD4+ T-lymphocytes is important in the management HIV. However, standard laboratory systems based on flow cytometry are expensive, complicated, and thus unavailable to most resource-limited settings where a low-cost fully automated point-of-care CD4 testing system required. In attempts address this issue, study was conducted validate Alere PIMA test.Duplicate values absolute number 203 HIV-infected blood samples obtained using were compared with two predicate single-platform...

In infectious diseases, extracellular vesicles (EVs) released from a pathogen or pathogen-infected cells can transfer pathogen-derived biomolecules, especially proteins, to target and consequently regulate these cells. For example, malaria is an important tropical disease caused by Plasmodium spp. Previous studies have identified the roles of falciparum-infected red blood cell-derived EVs (Pf-EVs) in pathogenesis, activation, modulation host immune responses. This study investigated...

Extracellular vesicles (EVs) released from pathogenic protozoans play crucial roles in host-parasite communication and disease pathogenesis. Naegleria fowleri is a free-living protozoan causing primary amoebic meningoencephalitis, fatal the central nervous system. This study aims to explore of N. fowleri-derived EVs (Nf-EVs) host-pathogen interactions using THP-1 cell line as model. The Nf-EVs were isolated trophozoite culture supernatant sequential centrifugation characterized by...

Abstract The CoronaVac (Sinovac Biotech) and ChAdOx1(Oxford-AstraZeneca) are two widely used COVID-19 vaccines. We examined the immunogenicity of four booster vaccine: BBIBP-CorV (Sinopharm Biotech), ChAdOx1, 30μg-BNT162b2 15μg-BNT162b2 (Pfizer-BioNTech), in healthy adults who received a two-dose or ChAdOx1 8-12 weeks earlier. Among 352 participants (179 173 participants), 285 (81%) were female, median age was 39(IQR: 31-47) years. 98%(175/179) 99%(172/173) Coronavac remained seropositive at...

Absolute CD4+ T-lymphocyte counts are used in the initiation and monitoring of antiretroviral therapy HIV-infected patients. Becton Dickinson's (BD) FACSCount system was introduced 12 years ago as a dedicated instrument for enumeration absolute T-lymphocytes. However, this does not provide percent that is required parameter pediatric We evaluated new BD CD4 software reagents simultaneous T-lymphocytes blood.Percent 149 blood samples were determined using system. Results compared between...

Background Nonvalvular atrial fibrillation (AF) is the most common cardiac arrhythmia, and it associated with prothrombotic state. Circulating microparticles (cMPs) are membrane vesicles that shed from many cell types in response to activation apoptosis. Several studies reported cMPs may play a role hypercoagulable state can be observed patients AF. The aim of this study was determine levels total characterize their cellular origins AF patients. Methods Atotal 66 33 healthy controls were...

Enumeration of CD4+ T lymphocytes is important in management HIV-infected patients. However, CD4 testing by current gold standard bead-based flow cytometer (FCM) system expensive for developing countries. This study compared 2 affordable volumetric FCMs with the 3 predicate FCM systems. T-lymphocyte counts on blood samples from 150 HIV-1-infected Thai patients were determined parallel 5 systems: single-platform systems, Guava and CyFlow(green); systems (2-color FACSCount TriTEST/TruCOUNT...

Upon searching plant extracts for inhibitors of the Ca2+ signaling pathway using zds1Δ-yeast proliferation based assay, a crude rhizome extract Boesenbergia pandurata was found to be strongly positive, and from this pinostrobin, alpinetin, pinocembrin chalcone were isolated as active components. Further biochemical experiments confirmed that pinostrobin possesses inhibitory activity on signals involved in control G2/M phase cell cycle progression Saccharomyces cerevisiae.

Abstract The appropriate COVID-19 booster vaccine following inactivated or adenoviral vector vaccination is unclear. We evaluated the safety and immunogenicity of different vaccines, (BBIBP-CorV), chimpanzee (ChAdOx1), mRNA (BNT162b2 at full (30 µg), half (15 µg) dose) in healthy adults who received 2-dose primary series either (CoronaVac) ChAdOx1 8-12 weeks earlier. Overall, adverse events for all vaccines were mild moderate. Two post-booster dose, neutralising antibody titres against Delta...

Affordable, easy-to-use, and reliable CD4(+) T lymphocyte enumeration systems are needed in resource-constrained settings to monitor HIV.A simple image cytometer was used count fluorescently labeled CD8(+) lymphocytes from CD3 immunomagnetically selected cells on blood specimens of 460 HIV-1-infected patients Siriraj Hospital, Bangkok, Thailand. Results were compared with flow cytometry (FCM).CD4(+) counts by comparable (R > or = 0.97) those the FACSCount FACScan a bias 7.3 9.1%,...

Mangosteen ( Garcinia mangostana L.) a tropical fruit, has been used in traditional medicine. A frequently part of mangosteen is the pericarp, containing high content xanthones. α-Mangostin, one major xanthone derivatives, exhibits variety actions, including antimicrobial, antioxidant, cytotoxic and antitumor; however, its function on immune system still equivocal. This study aimed to examine immunomodulatory activities α-mangostin lymphocyte lineage cytokine production human peripheral...

Abstract Water-soluble polysaccharides were isolated from the tubers of Butea superba Roxb. and Pueraria candollei Wall. Ex Benth. var. mirifica (Shaw et Suvat.) C. Niyomdham, leaves Centella asiatica (L.) Urb, Ocimum basilicum L., Psidium guajava Andrographis paniculata (Burn. f.) Nees, stems Cymbopogon citratus (Stapf ExG), fruits Scaphium scaphigerum. The immunological impacts on T-lymphocyte proliferation in vitro was investigated by flow cytometric (immunofluorescence) analysis using...

Background: The BD FACSPresto ™ system uses capillary and venous blood to measure CD4 absolute counts (CD4), %CD4 in lymphocytes, hemoglobin (Hb) approximately 25 minutes. cell count is used with portable counters resource-limited settings manage HIV/AIDS patients. A method comparison was performed using samples from seven clinical laboratories five countries. assessed for variability between laboratory, instrument/operators, cartridge lots within-run at four sites. Methods: Samples were...

Abstract Importance Inactivated vaccine (CoronaVac) and chimpanzee adenovirus-vector (ChAdOx1) have been more available in resource-limited settings. However, the data comparing between these two vaccines same setting are limited. Objectives To determine adverse events (AEs) immunogenicity of CoronaVac ChAdOx1 health care workers (HCWs). Design This prospective study was conducted from February to July 2021. Setting A single center, university-based tertiary center Bangkok. Participants...

A quick, reliable, and reproducible biological assay to distinguish individuals with possible life-threatening risk following radiological or nuclear incidents remains a quest in biodosimetry. In this paper, we examined the use of γ-H2AX as an early dose estimation for rapid triage based on both flow cytometry image analyses. experiment, whole blood from 11 donors was irradiated ex vivo inside water phantom by gamma rays Co-60 at 0.51 Gy/min. After lysis red cells, white cells were collected...

CD4(+) T-lymphocyte count is an important marker in management of HIV-1-infected patients. The standard single-platform (SP) flow cytometric (FCM) testing that uses the known reference microbeads expensive; more affordable alternatives are therefore needed. We evaluated use glutaraldehyde-fixed chicken red blood cells (CRBCs) as counting beads alternative for enumerating counts 87 Linear regression analyses revealed excellent correlation SP FCM using CRBCs with bead-based method...

Abstract Flow cytometric assays for quantifying polymorphonuclear leucocyte (PMN) function involve peripheral blood fractionation methods. However, most are of limited use conditions like pediatrics and thalassemia, as they may require large volumes or complicated by red cell contamination. In whole assay, 500‐μl aliquots normal thalassemic were incubated with bis‐carboxyethyl‐carboxyfluorescein pentaacetoxymethylester (BCECF‐AM)‐labeled Candida albicans cells phycoerythrin‐conjugated...

Immunization with a pandemic influenza A H1N1 2009 was recommended for HIV-infected patients. However, there is limited information concerning the impact of immunization this vaccine on immune activation and HIV viral replication. In study, 45 children adolescents receiving antiretroviral therapy were immunized 2-dose series nonadjuvated monovalent upon enrollment approximately 1 month later. Immunogenicity determined by haemagglutination inhibition assay. The level identification CD38...

The aim of this study was to evaluate the performance automated impedance platelet counts by Beckman Coulter LH780 (PLT-LH), Sysmex XN-3000 (PLT-XNi) and fluorescence method (PLT-F) in patients with acute leukemia. Blood specimens were subjected measurements evaluated methods then compared against international reference (IRM). Eighty-two blood included. Bland–Altman plots differences between IRM demonstrated mean biases PLT-LH, PLT-XNi PLT-F 9 × 109/L, 11 109/L 2 respectively. For...