A5087154078- Cancer Genomics and Diagnostics
- Advanced Breast Cancer Therapies
- Estrogen and related hormone effects
- BRCA gene mutations in cancer
- Breast Cancer Treatment Studies
- HER2/EGFR in Cancer Research
- Natural product bioactivities and synthesis
- Cancer-related Molecular Pathways
- Cancer Treatment and Pharmacology
- Ferroptosis and cancer prognosis
- Neutropenia and Cancer Infections
- Cancer Immunotherapy and Biomarkers
- Thyroid Cancer Diagnosis and Treatment
- Phytoestrogen effects and research
- Toxin Mechanisms and Immunotoxins
- Chemotherapy-related skin toxicity
- Phytochemistry and Biological Activities
- Lung Cancer Treatments and Mutations
- PI3K/AKT/mTOR signaling in cancer
- Cancer Cells and Metastasis
- Chronic Lymphocytic Leukemia Research
- MicroRNA in disease regulation
- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- Neuroendocrine Tumor Research Advances
Kumamoto City Hospital
2022-2024
Hokkaido University Hospital
2020-2023
Roswell Park Comprehensive Cancer Center
2019-2020
Daido University
2020
Kumamoto University
1988-2018
Kasugai Municipal Hospital
2018
Kyushu University
2018
Fukuoka University
2018
Kumamoto University Hospital
2018
Japanese Red Cross Kumamoto Hospital
2013
// Aiko Sueta 1 , Yutaka Yamamoto Mai Tomiguchi Takashi Takeshita Mutsuko Yamamoto-Ibusuki 2 and Hirotaka Iwase Department of Breast Endocrine Surgery, Kumamoto University Graduate School Medical Sciences, 860-8556, Japan Molecular-Targeting Therapy for Cancer, Hospital, Correspondence to: Yamamoto, email: ys-yama@triton.ocn.ne.jp Keywords: exosome, microRNA, breast cancer, circulating biomarker, prognostic factor Received: April 19, 2017     Accepted: June 28, Published:...
Two new isoflavone glycosides, the 8-C-apiosyl (1→6) glucosides of daidzein and genistein, were isolated from roots voluble stems Pueraria lobata (WILLD.) OHWI. Thirteen known isoflavonoids related compounds also found.
The basal-like breast cancer, a new category of cancer associated with poor prognosis and possibly unique chemosensitivity, is current topic in the field. Evidence from multiple sources strongly indicate that impairment BRCA1 pathways responsible for this phenotype, implying importance not only familial cancers but also sporadic cancers. acts as hub protein coordinates diverse range cellular to maintain genomic stability. participates supercomplexes execute its tasks and, most complexes,...
PIK3CA is an oncogene that encodes the p110α component of phosphatidylinositol 3-kinase (PI3K); it second most frequently mutated gene following TP53 gene. In clinical setting, mutations may have favorable prognostic value for hormone receptor-positive breast cancer patients and, during past few years, cell-free DNA (cfDNA) attracted attention as a potential noninvasive biomarker cancer. However, there are reports on implications TNBC patients. We investigated major mutation status cfDNA...
// Takashi Takeshita 1 , Yutaka Yamamoto Mutsuko Yamamoto-Ibusuki 2 Toko Inao Aiko Sueta Saori Fujiwara Yoko Omoto 1, 3 Hirotaka Iwase Department of Breast and Endocrine Surgery, Graduate School Medical Science, Kumamoto University, Chuo-ku, Kumamoto, 860–8556, Japan Molecular-Targeting Therapy for Cancer, University Hospital, Kyoto Prefectural Medicine, Hirokoji Agaru, Kawaramachi-dori, Kamigyo-ku, Kyoto, 602–0841, Correspondence to: Iwase, email: hiwase@kumamoto-u.ac.jp...
The measurement of ESR1 and PIK3CA mutations in plasma cell-free DNA (cfDNA) has been studied as a non-invasive method to quickly assess monitor endocrine therapy (ET) resistant metastatic breast cancer (MBC) patients.The subjects this retrospective study were total 185 samples from 86 estrogen receptor-positive BC patients, which 151 69 MBC patients 34 17 primary (PBC) patients. We developed multiplex droplet digital PCR assays verify the clinical significance both snapshot serially these...
Abstract The fact that 20–40% of all breast cancer (BC) patients develop recurrence when 5 year survival is 90% strongly suggests late recurrence, i.e. more than years after diagnosis, the remaining challenge to decrease absolute number BC deaths. Better understanding an essential first step address this issue. We hypothesized primary tumors with a distinctive tumor immune microenvironment will recurrence. Accordingly, we evaluated relationship between timing clinical factors, gene...
Recently, research into the development of new targeted therapies has focused on specific genetic alterations to create advanced, more personalized treatment. One target genes, fibroblast growth factor receptor‐1 ( FGFR 1 ), been reported be amplified in estrogen receptor ER )‐positive subtype breast cancer, and is considered one possible mechanism endocrine resistance through cross‐talk between signaling. We performed a comprehensive analysis at levels gene copy number, transcript protein...
DNA abnormalities are used in inclusion criteria of clinical trials for treatments with specific targeted molecules. MYC is one the most powerful oncogenes and known to be associated triple-negative breast cancer (TNBC). Its amplification often part DNA-sequencing panels under assumption reflecting upregulated signaling. However, it remains unclear if a surrogate its Thus, we investigated difference between mRNA high expression TNBCs utilizing publicly available cohorts. amplified tumors...
A new triterpenoidal saponin, 3-O-[α-L-rhamnopyanosyl(1→2)-α-L-arabinopyranosyl(1→2)-β-D-glucuronopyranosyl]sophoradio (I), was isolated from the flowers of Pueraria lobata (WILLD.) OHWI (Leguminosae). Its structure elucidated on basis chemical and physicochemical evidence. In addition, a known oligoglycoside kaikasaponin III (II) both leaves this plant, together with several aromatic compounds.
ESR1 mutation in circulating cell-free DNA (cfDNA) is emerging as a noninvasive biomarker of acquired resistance to endocrine therapy, but there paucity data comparing the status gene cfDNA with that its corresponding tumor tissue. The objective this study validate degree concordance mutations between plasma and tissue.ESR1 ligand-binding domain Y537S, Y537N, Y537C, D538G were analyzed using droplet digital PCR 35 patients metastatic breast cancer (MBC) (35 tissue samples 67 samples).Of...
The phosphoinositide-3-kinase (PI3K) pathway is commonly deregulated in breast cancer through several mechanisms, including PIK3CA mutation and loss of phosphatase tensin homolog (PTEN) inositol polyphosphate 4-phosphatase-II (INPP4B). We aimed to evaluate the predictive relevance these biomarkers trastuzumab efficacy HER2-positive disease. evaluated effect 43 patients with HER2-overexpression who received neoadjuvant treatment. was examined by direct sequencing digital PCR assay, copy...
// Saori Fujiwara 1,* , Mutsuko Yamamoto-Ibusuki Yutaka Yamamoto 1,2 Satoko 1 Mai Tomiguchi Takashi Takeshita Mitsuhiro Hayashi Aiko Sueta and Hirotaka Iwase Department of Breast Endocrine Surgery, Graduate School Medical Sciences, Kumamoto University, 1-1-1 Honjo, Chuo-ku, 860-8556, Japan 2 Molecular Targeting Therapy for Cancer, * These Authors are equally contributed to this work Correspondence: Iwase, email: Keywords : HER4, 4ICD, Splicing variants, cancer, therapy, Prognosis Received...
In the general rule, Puerariae Flos or it's combination drugs are used in traditional medicinal system for counteraction to drinking among Japanese and Chinese therapy. One of such is Kakkakaiseito. Here we report results investigation on some pharmacological actions including alcoholic metabolism. The experiments were carried out confirm its actual effect alcohol, acetaldehyde ketone body metabolism blood alteration behaviour pattern mice. Drugs this study methanolic extract (PF-ME),...
Estrogen signals play an important role in the phenotype of estrogen receptor-positive breast cancer. However, comprehensive analyses effect responsiveness to on tumor microenvironment and survival large cohorts primary cancer patients have been lacking. We aimed test hypothesis that reactivity affects gene expression immune cell infiltration profiles survival.A total 3,098 cases were analyzed: 1,904 from Molecular Taxonomy Breast Cancer (METABRIC) cohort, 1,082 The Genome Atlas (TCGA) 112...
From Abri Herba, the whole plant of Abrus cantoniensis (Leguminosae), ten new oleanene saponins together with thirteen known onew were isolated. Among them, four called abrisaponins L, A, D1 and Ca have been elucidated to be 3-O-α-L-rhamnopyranosyl-(1→2)-β-D-galactopyranosyl-(1→2)-β-D-glucuronopyranosyl (designated as β-fabatriosyl) abrisapogenol L (1), 3-O-β-fabatriosyl A (2), D (3), cantoniensistriol (4), respectively.
The cancer-immunity cycle (CIC) is a series of self-sustaining stepwise events to fight cancer growth by the immune system. We hypothesized that immunofunctional phenotyping represent malfunction CIC clinically relevant in breast (BC). Total 2979 BC cases; 1075 from TCGA cohort, 1904 METABRIC cohort were analyzed. phenotype was classified as follows: hot T-cell infiltrated (HTI), high cytolytic activity (CYT), Cold (CTI), frequency CD8+ T cells and low CYT, non-inflamed, CYT. analysis tumor...
Breast cancer treatment often employs DNA double-strand breaks (DSBs), such as that induced by irradiation or anticancer agents. Ubiquitination is required at the site of damage and plays a crucial role in DSB repair pathway. We investigated effect proteasome inhibitors on pathway after exposure to chemotherapeutic agents examined its correlation with cytotoxicity. Cells were exposed for 1 h damage-inducing After damage, nuclear foci formation conjugated ubiquitin (Ub-foci) cell viability...
The combination of cyclin-dependent kinase 4/6 inhibitors and endocrine therapy is a standard treatment for hormone receptor (HR)-positive/human epidermal growth factor 2 (HER2)-negative metastatic breast cancer (MBC); however, their toxicities financial burden are major issues, especially prolonged treatment. We investigated fulvestrant plus palbociclib in patients with HR-positive MBC resistant to monotherapy.Patients who initially received as first- or second-line were assigned group A....