A5087245344- X-ray Spectroscopy and Fluorescence Analysis
- Enzyme function and inhibition
- Protein Structure and Dynamics
- Enzyme Structure and Function
- Hormonal and reproductive studies
- Monoclonal and Polyclonal Antibodies Research
- Electron and X-Ray Spectroscopy Techniques
- Enzyme Catalysis and Immobilization
- Protein purification and stability
- Diet, Metabolism, and Disease
- Steroid Chemistry and Biochemistry
- Nuclear Physics and Applications
- Mass Spectrometry Techniques and Applications
- Pancreatic function and diabetes
- Polyamine Metabolism and Applications
- Biochemical and Molecular Research
- Atomic and Molecular Physics
- Enzyme Production and Characterization
- Amino Acid Enzymes and Metabolism
- Chemical Reactions and Isotopes
- Cholinesterase and Neurodegenerative Diseases
- Chemical Thermodynamics and Molecular Structure
- Renin-Angiotensin System Studies
- Blood Coagulation and Thrombosis Mechanisms
- RNA and protein synthesis mechanisms
Tampere University
2013-2024
Tampere University of Applied Sciences
2011-2013
Institute of Biomedical Science
2013
Tampere University Hospital
2007-2011
University of Eastern Finland
2002-2006
Joensuu Science Park
2002
Helsinki Institute of Physics
1976-1985
Carbonic anhydrase IX (CA IX) is an exceptional member of the CA protein family; in addition to its classical role pH regulation, it has also been proposed participate cell proliferation, adhesion, and tumorigenic processes. To characterize biochemical properties this membrane protein, two soluble recombinant forms were produced using baculovirus-insect expression system. The proteins consisted either catalytic domain only form) or extracellular domain, which included both proteoglycan...
Zinc metalloproteins are one of the most abundant and structurally diverse proteins in nature. In these proteins, Zn(II) ion possesses a multifunctional role as it stabilizes fold small zinc fingers, catalyzes essential reactions enzymes all six classes, or assists formation biological oligomers. Previously, number database surveys have been conducted on to gain broader insights into their rich coordination chemistry. However, many suffer from severe flaws misinterpretations otherwise...
Twelve members of the family 11 xylanases, including both mesophilic and thermophilic proteins, were studied using molecular dynamics (MD). Simulations xylanases carried out in an explicit water environment at four different temperatures, 300, 400, 500 600 K. A difference thermotolerance between became clear: endured heat higher simulation temperatures better than ones. The unfolding pathways seemed to be similar for all simulations regardless protein. initiates N-terminal region or...
Background: The main purpose of the study was to establish whether essential components renin-angiotensin system (RAS) exist in human aqueous humor. Methods: Forty-five patients ≥ 60 (74±7) years age undergoing cataract surgery at Tampere University Hospital were randomly selected for prospective study. exclusion criterion use oral antihypertensive medicine acting via system. Aqueous humor samples taken beginning normal extraction. frozen and stored -80 °C. concentrations intraocular...
Rhizobium etli CFN42 is a symbiotic nitrogen-fixing bacterium of the common bean Phaseolus vulgaris. The plasmid p42d R. comprises gene encoding putative (strept)avidin-like protein, named rhizavidin. amino acid sequence identity rhizavidin in relation to other known avidin-like proteins 20–30%. residues involved (strept)avidin–biotin interaction are well conserved structural and functional properties were carefully studied, we found that shares characteristics with bradavidin, streptavidin...
Purpose: An active local renin-angiotensin system (RAS) has recently been found in the human eye. The aim of present study was to compare activities central RAS enzymes (ACE1 and 2) porcine ocular tissues, morphologically physiologically close In addition, effects three ACE-inhibitory tripeptides on these were evaluated. Methods: Enucleated fresh eyes used. Activities ACE1 ACE2 their inhibition by bioactive (Ile-Pro-Pro, Val-Pro-Pro, Leu-Pro-Pro) as well a standard ACE-inhibitor captopril...
Deacetylation of histones is carried out by a corepressor complex in which Sin3A an essential scaffold protein. Two proteins this complex, the Sin3A-associated SAP30L and SAP30, have previously been suggested to function as linker molecules between various corepressors. In report, we demonstrate new functions for human SAP30 showing that they can associate directly with core well naked DNA. A zinc-coordinating structure necessary DNA binding, one consequence bending We provide evidence...
Unnatural amino acids (UAAs) offer significant promise in a wide range of applications, including drug discovery, the custom design peptides and proteins, their utility use as markers for monitoring molecular interactions biological research. The synthesis UAAs presents formidable challenge can be classified into two primary categories: enzymatic chemical synthesis. Notably, route, specifically asymmetric synthesis, emerges an attractive method procuring enantiopure with high efficiency,...
A highly selective, high affinity recombinant anti-testosterone Fab fragment has been generated by stepwise optimization of the complementarity-determining regions (CDRs) random mutagenesis and phage display selection a monoclonal antibody (3-C4F5). The best mutant (77 Fab) was obtained evaluating additivity effects different independently selected CDR mutations. 77 contains 20 mutations about 40-fold increased (K d = 3 × 10−10m) when compared with wild-type (3-C4F5) Fab. To obtain...
Conversion of hydrogen carbonate to formate by mutants Candida methylica (CmFDH) and Chaetomium thermophilum (CtFDH) dehydrogenases (FDHs) was studied. Hydrogen is not the primary substrate for hydride transfer reaction in FDHs. The chosen mutations were selected so that enzyme activity could remain at an adequate level. In CtFDH, mutation Asn120Cys active site inactivated (oxidation) but increased specific (reduction) as a function concentration. CtFDH 6.5-fold KM, indicating binding...
STAT1 is an essential transcription factor for interferon-γ-mediated gene responses. A distinct sumoylation consensus site (ψKxE) 702IKTE705 localized in the C-terminal region of STAT1, where Lys703 a target PIAS-induced SUMO modification. Several studies indicate that has inhibitory role on STAT1-mediated expression but molecular mechanisms are not fully understood.Here, we have performed structural and functional analysis STAT1. We show deconjugation by SENP1 enhances transcriptional...
High-resolution oxygen K x-ray spectra have been obtained from a number of ionic insulators. A distortion the 1${s}^{\mathrm{\ensuremath{-}}1?}$(${\mathrm{hyV}}^{\mathrm{\ensuremath{-}}1}$ emission band due to initial 1s hole is observed and related variation in 1${s}^{\mathrm{\ensuremath{-}}1}$${V}^{\mathrm{\ensuremath{-}}1?}$(${\mathrm{hyV}}^{\mathrm{\ensuremath{-}}2}$ satellite structure as function bonding character. shake model which considers influence crystal environment on relaxation...
Our previous microarray study showed that the non-specific cytotoxic cell receptor protein 1 (Nccrp1) transcript is significantly upregulated in gastric mucosa of carbonic anhydrase IX (CA IX)-deficient (Car9−/−) mice. In this paper, we aimed to characterize human NCCRP1 and elucidate its relationship CA IX. Recombinant was expressed Escherichia coli, a novel polyclonal antiserum raised against purified full-length protein. Immunocytochemistry intracellularly, even though it has previously...
Carbonic anhydrase isoenzyme VI (CA VI) plays an important role in the homeostasis of oral tissues participating processes taste, protection dental against loss minerals, caries, and possibly formation calculus periodontal disease. This study aimed to verify correlation between changes expression activity human salivary carbonic genetic polymorphisms its gene (CA6). The population consisted 182 healthy volunteers (female male, aged 18-22). Samples total saliva were assayed for CA...
Acetaldehyde can generate modifications in several proteins, such as carbonic anhydrase (CA) II. In this study, we extended vitro investigations on acetaldehyde adduct formation by focusing the other human cytosolic CA enzymes I, III, VII, and XIII. High-resolution mass spectrometric analysis indicated that most efficiently formed covalent adducts with II The binding of up to 19 acetaldehydes is probably attributable high number lysine residues (n = 24) located mainly surface enzyme...
The milk casein-derived bioactive tripeptides isoleucine-proline-proline (IPP) and valine-proline-proline (VPP) have been shown to prevent development of hypertension in animal models lower blood pressure moderately hypertensive subjects most but not all clinical trials. Inhibition angiotensin-converting enzyme 1 (ACE-1) has suggested as the explanation for these antihypertensive beneficial vascular effects. Previously, human umbilical vein endothelial cells (HUVEC) used test ACE-1...
Abstract Background Acetaldehyde, the first metabolite of ethanol, can generate covalent modifications proteins and cellular constituents. However, functional consequences such modification remain poorly defined. In present study, we examined acetaldehyde reaction with human carbonic anhydrase (CA) isozyme II, which has several features that make it a suitable target protein: It is widely expressed, its enzymatic activity be monitored, structural catalytic properties are known, contains 24...
Interactions and force resistance of different β-integrin–talin complexes were analysed in a set steered molecular dynamics simulations.