A5000739218- Advanced MRI Techniques and Applications
- MRI in cancer diagnosis
- Electron Spin Resonance Studies
- Cardiac Imaging and Diagnostics
- Advanced Neuroimaging Techniques and Applications
- Advanced NMR Techniques and Applications
- Virus-based gene therapy research
- Cardiovascular Function and Risk Factors
- Aortic Disease and Treatment Approaches
- Aortic aneurysm repair treatments
- Lanthanide and Transition Metal Complexes
- Cardiac Valve Diseases and Treatments
- Radiomics and Machine Learning in Medical Imaging
- Angiogenesis and VEGF in Cancer
- NMR spectroscopy and applications
- Ultrasound and Hyperthermia Applications
- Osteoarthritis Treatment and Mechanisms
- RNA Interference and Gene Delivery
- Prostate Cancer Diagnosis and Treatment
- MicroRNA in disease regulation
- Cardiomyopathy and Myosin Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Neuroinflammation and Neurodegeneration Mechanisms
- Aortic Thrombus and Embolism
- Traumatic Ocular and Foreign Body Injuries
Oulu University Hospital
2018-2024
University of Eastern Finland
2013-2024
University of Oulu
2018-2024
Kuopio University Hospital
2005-2022
Institute for Molecular Medicine Finland
2006-2021
Institute for Molecular Science
2008-2017
Universidad de Granada
2016
University of Minnesota
2008-2011
Resonance Research (United States)
2008-2011
Eastern Finland Laboratory Center
2008
Type 2 diabetes mellitus (T2DM) is a major risk factor for heart disease. Mortality rates after myocardial infarction (MI) are significantly increased in T2DM patients because of dysfunctional left ventricle (LV). However, molecular pathways underlying accelerated failure (HF) MI remain unclear. We investigated the mechanisms by inducing well-established model and control mice. Cardiac imaging revealed decreased global ventricular ejection fraction parallel with mortality mice compared...
Background— For clinically relevant proangiogenic therapy, it would be essential that the growth of whole vascular tree is promoted. Vascular endothelial factor (VEGF) well known to induce angiogenesis, but its capability promote larger vessels controversial. We hypothesized blood flow remodels during VEGF gene therapy and may contribute large vessels. Methods Results— Adenoviral (Ad) or LacZ control transfer was performed in rabbit hindlimb semimembranous muscles with without ligation...
Rhizobium etli CFN42 is a symbiotic nitrogen-fixing bacterium of the common bean Phaseolus vulgaris. The plasmid p42d R. comprises gene encoding putative (strept)avidin-like protein, named rhizavidin. amino acid sequence identity rhizavidin in relation to other known avidin-like proteins 20–30%. residues involved (strept)avidin–biotin interaction are well conserved structural and functional properties were carefully studied, we found that shares characteristics with bradavidin, streptavidin...
Abstract A new method to measure rotating frame relaxation and create contrast for MRI is introduced. The technique exploits along a fictitious field (RAFF) generated by amplitude‐ frequency‐modulated irradiation in subadiabatic condition. Here, RAFF demonstrated using radiofrequency pulse based on sine cosine amplitude frequency modulations of equal amplitudes, which gives rise stationary magnetic doubly frame. According dipolar theory, the time constant ( T ) was found differ from...
Heart has a wide lymphatic network but the importance of cardiac system in heart diseases remained unclear. Vascular Endothelial Growth Factor Receptor 3 (VEGFR3) is key molecule development and maintenance vessels. Here we characterized role VEGFR3 healthy hearts after myocardial infarction (MI) by using sVEGFR3 transgenic mice expressing soluble decoy under K14 promoter Chy which have an inactivating mutation gene. Cardiac vessels were significantly dilated when compared to controls....
Drug carrier systems based on mesoporous inorganic nanoparticles generally face the problem of fast clearance from bloodstream thus failing in passive and active targeting to cancer tissue. To address this problem, a specific dual PEGylation (DPEG) method for silicon (PSi) was developed studied vitro vivo. The DPEG coating changed significantly behavior vivo, increasing circulation half-life 1 241 min. Furthermore, accumulation coated particles mainly taking place spleen whereas uncoated...
Objectives: In thoracic aortic aneurysm (TAA) of the ascending aorta (AA), AA is progressively dilating due to weakening wall. Predicting and preventing dissections ruptures in TAA continues be challenging, more accurate assessment dilatation, identification high-risk patients, timing repair surgery are required. We investigated whether wall shear stress (WSS) predicts pathological biomechanical changes TAA. Methods: The study included 12 patients with bicuspid (BAV) 20 tricuspid valve...
MRI relaxation measurements are performed in the presence of a fictitious magnetic field recently described technique known as RAFF (Relaxation Along Fictitious Field). This method operates 2(nd) rotating frame (rank n = 2) by using nonadiabatic sweep radiofrequency effective to generate field. In present study, is extended for generating contrasts frames ranks 1 ≤ 5. The developed entitled rank (RAFFn).RAFFn pulses were designed fields that allow locking magnetization n. Contrast generated...
Purpose Brain myelin plays an important role in normal brain function. Demyelination is involved many degenerative diseases, thus quantitative imaging of has been under active investigation. In previous work, we demonstrated the capability method known as Relaxation Along a Fictitious Field (RAFF) rotating frame rank n (RAFFn) to provide image contrast between white and gray matter human rat brains. Here, evidence pointing being major source this contrast. Methods RAFFn relaxation time...
Abstract Oncolytic viruses are a promising tool for treatment of cancer. We studied an oncolytic Semliki Forest virus (SFV) vector, VA7, carrying the enhanced green fluorescent protein gene (EGFP), as novel virotherapy candidate against unresectable osteosarcoma. The efficiency and characteristics VA7-EGFP were compared with widely adenovirus, Ad5Δ24, both in vitro vivo. resulted more rapid oncolysis was efficient at low multiplicities infection (MOI) when Ad5Δ24 vitro. Yet, MG-63 cells,...
Abstract Longitudinal relaxation time in the rotating frame ( T 1 ρ ) was measured using continuous wave irradiation normal and infarcted mouse myocardium vivo. Significant increase found after 7 days of infarction when compared with reference or before infarction. Cine MRI histology were performed to verify severity The course infarct fits better granulation scar tissue formation than necrosis edema. results study show that could potentially be a noninvasive quantitative marker for...
Herein, we report the use of biodegradable nanoparticles (NPs) containing perfluoro-1,5-crown ether (PFCE), a fluorine-based compound (NP170-PFCE) with capacity to track cells in vivo by magnetic ressonance imaging (MRI) and efficiently release miRNA. NP170-PFCE complexed miRNAs accumulate whitin cell's endolysosomal compartment interact higher frequency argonaute2 (Ago2) GW182 proteins, which are involved biological action miRNAs, than commercial complexes formed reagents miRNA, turn cell...
ABSTRACT Several laboratory and rotating frame quantitative MRI parameters were evaluated compared for detection of changes in articular cartilage following selective enzymatic digestion. Bovine osteochondral specimens subjected to 44 h incubation control medium or collagenase chondroitinase ABC induce superficial collagen proteoglycan (glycosaminoglycan) alterations. The samples scanned at 9.4 T 1 , Gd (dGEMRIC), 2 adiabatic ρ continuous‐wave RAFF2 sat relaxation times magnetization...
Abstract We evaluated the therapeutic potential of replication competent vector VA7‐EGFP, which is based on avirulent Semliki Forest virus (SFV) strain A7 (74) carrying EGFP marker gene in an orthotopic lung cancer tumor model nude mice. have previously shown that this oncolytic destroys cells efficiently vitro and vivo (in subcutaneous model). Tumor growth animals with orthotopically implanted adenocarcinoma (A549) were monitored during study small animal CT. show locally administered...
"Epigenetherapy" alters epigenetic status of the targeted chromatin and modifies expression endogenous therapeutic gene. In this study we used lentiviral in vivo delivery small hairpin RNA (shRNA) into hearts a murine infarction model. shRNA complementary to promoter vascular endothelial growth factor (VEGF-A) was able upregulate VEGF-A expression. Histological multiphoton microscope analysis confirmed effect transduced hearts. Magnetic resonance imaging (MRI) showed that infarct size...