A5012959772- Atherosclerosis and Cardiovascular Diseases
- Aortic Disease and Treatment Approaches
- Aortic aneurysm repair treatments
- Vascular Tumors and Angiosarcomas
- Vascular Malformations and Hemangiomas
- Cardiac Valve Diseases and Treatments
- Single-cell and spatial transcriptomics
- Hippo pathway signaling and YAP/TAZ
- RNA modifications and cancer
- Angiogenesis and VEGF in Cancer
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- Genetic Associations and Epidemiology
- Platelet Disorders and Treatments
- Galectins and Cancer Biology
- Soft tissue tumor case studies
- Infectious Aortic and Vascular Conditions
University of Eastern Finland
2020-2024
Abstract The BMP/TGFβ-Smad, Notch and VEGF signaling guides formation of endothelial tip stalk cells. However, the crosstalk bone morphogenetic proteins (BMPs) vascular growth factor receptor 2 (VEGFR2) has remained largely unknown. We demonstrate that BMP family members regulate VEGFR2 signaling, act via TAZ-Hippo pathway. BMPs were found to be regulated after gene transfer in C57/Bl6 mice a porcine myocardial ischemia model. 2/4/6 identified as endothelium-specific targets VEGF. BMP2...
Coronary artery disease (CAD) is a pandemic where up to half of the risk explained by genetic factors. Advanced insights into basis CAD require deeper understanding contributions different cell types, molecular pathways, and genes heritability. Here, we investigate biological diversity atherosclerosis-associated states interrogate their contribution using single-cell bulk RNA sequencing (RNA-seq) mouse human lesions. We identified 12 disease-associated that characterized further gene set...
Abstract Aims Vascular smooth muscle cells (SMCs) and their derivatives are key contributors to the development of atherosclerosis. However, studying changes in SMC gene expression heterogeneous vascular tissues is challenging due technical limitations high cost associated with current approaches. In this paper, we apply translating ribosome affinity purification sequencing profile SMC-specific directly from tissue. Methods results To facilitate translatome analysis, generated SMCTRAP mice,...
Objectives: In thoracic aortic aneurysm (TAA) of the ascending aorta (AA), AA is progressively dilating due to weakening wall. Predicting and preventing dissections ruptures in TAA continues be challenging, more accurate assessment dilatation, identification high-risk patients, timing repair surgery are required. We investigated whether wall shear stress (WSS) predicts pathological biomechanical changes TAA. Methods: The study included 12 patients with bicuspid (BAV) 20 tricuspid valve...
Previous studies have demonstrated that the expression of several lysine (K)-specific demethylases (KDMs) is induced by hypoxia. Here, we sought to investigate exact mechanisms underlying this regulation and its functional implications for endothelial cell function, such as angiogenesis. Approach Results: We analyzed changes KDMs under hypoxia modulation HIF (hypoxia-inducible factor) using GRO-Seq RNA-Seq in cells. provide evidence majority are at level nascent transcription mediated action...
Sporadic venous malformation (VM) and angiomatosis of soft tissue (AST) are benign, congenital vascular anomalies affecting vasculature. Depending on the size location lesion, symptoms vary from motility disturbances to pain disfigurement. Due high recurrence lesions, more effective therapies needed. As targeting stromal cells has been an emerging concept in anti-angiogenic therapies, here, by using VM/AST patient samples, RNA-sequencing, cell culture techniques, a xenograft mouse model, we...
Abstract Coronary artery disease (CAD) is a pandemic where up to half of the risk explained by genetic factors. Advanced insights into basis CAD require deeper understanding contributions different cell types, molecular pathways and genes heritability. Here, we investigate biological diversity atherosclerosis associated states interrogate their contribution using single bulk RNA sequencing (RNA-Seq) mouse human lesions. We identified thirteen disease-associated which characterized further...
Background: Sporadic venous malformation (VM) and angiomatosis of soft tissue (AST) are benign, congenital vascular anomalies affecting vasculature. Depending on the size location lesion, symptoms vary from motility disturbances to pain disfigurement. Due high recurrence lesions more effective therapies needed. Methods: As targeting stromal cells has been an emerging concept in anti-angiogenic therapies, here, by using VM/AST patient samples, RNA-sequencing, cell culture techniques a...