A5019345986- Atherosclerosis and Cardiovascular Diseases
- Photodynamic Therapy Research Studies
- Porphyrin and Phthalocyanine Chemistry
- Virus-based gene therapy research
- Angiogenesis and VEGF in Cancer
- Viral Infectious Diseases and Gene Expression in Insects
- Viral gastroenteritis research and epidemiology
- Adipose Tissue and Metabolism
- Hippo pathway signaling and YAP/TAZ
- CAR-T cell therapy research
- Photochromic and Fluorescence Chemistry
- Nanoplatforms for cancer theranostics
- Pharmacological Effects and Assays
- Galectins and Cancer Biology
- Zebrafish Biomedical Research Applications
- Retinal Diseases and Treatments
- Receptor Mechanisms and Signaling
- RNA modifications and cancer
- Neuroinflammation and Neurodegeneration Mechanisms
University of Eastern Finland
2017-2024
Kuopio University Hospital
2019
Finland University
2019
Abstract The BMP/TGFβ-Smad, Notch and VEGF signaling guides formation of endothelial tip stalk cells. However, the crosstalk bone morphogenetic proteins (BMPs) vascular growth factor receptor 2 (VEGFR2) has remained largely unknown. We demonstrate that BMP family members regulate VEGFR2 signaling, act via TAZ-Hippo pathway. BMPs were found to be regulated after gene transfer in C57/Bl6 mice a porcine myocardial ischemia model. 2/4/6 identified as endothelium-specific targets VEGF. BMP2...
Abstract Aims Vascular smooth muscle cells (SMCs) and their derivatives are key contributors to the development of atherosclerosis. However, studying changes in SMC gene expression heterogeneous vascular tissues is challenging due technical limitations high cost associated with current approaches. In this paper, we apply translating ribosome affinity purification sequencing profile SMC-specific directly from tissue. Methods results To facilitate translatome analysis, generated SMCTRAP mice,...
Selective elimination of macrophages by photodynamic therapy (PDT) is a new and promising therapeutic modality for the reduction atherosclerotic plaques. m-Tetra(hydroxyphenyl)chlorin (mTHPC, or Temoporfin) may be suitable as photosensitizer this application, it currently used in clinic cancer PDT. In present study, mTHPC was encapsulated polymeric micelles based on benzyl-poly(ε-caprolactone)-b-methoxy poly(ethylene glycol) (Ben-PCL-mPEG) using film hydration method, with loading capacity...
In this paper we describe a straightforward supramolecular strategy to encapsulate silicon phthalocyanine (SiPc) photosensitizers (PS) in polymeric micelles made of poly(ε-caprolactone)-b-methoxypoly(ethylene glycol) (PCL-PEG) block copolymers. While PCL-PEG are promising nanocarriers based on their biocompatibility and biodegradability, the design our new PS favors encapsulation. particular, they combine two axial benzoyl substituents, each them carrying either three hydrophilic...
Biological sex has fundamental effects on mammalian heart physiology and pathogenesis. While it been established that female is a protective factor against most cardiovascular diseases (CVDs), this beneficial effect may involve pathways associated with cardiac energy metabolism. Our aim was to elucidate the role of transcriptional coactivator PGC-1α in dimorphism failure (HF) development.Here, we show mice deficient expression peroxisome proliferator-activated receptor gamma (PPAR-γ)...
Vascular endothelial growth factor-A (VEGF-A) is the master regulator of angiogenesis, vascular permeability and growth. However, its role in mature blood vessels still not well understood. To better understand VEGF-A adult vasculature, we generated a knockdown mouse model carrying doxycycline (dox)-regulatable short hairpin RNA (shRNA) transgene, which silences VEGF-A. The aim was to find critical level reduction for well-being vivo. In vitro, dox-inducible lentiviral shRNA vector decreased...
Biological bypass through induced angiogenesis by vascular endothelial growth factor D (VEGF-D) gene therapy (GT) is a new concept for the treatment of cardiac ischemia. Serotype 5 adenoviruses are used in clinical trials transferring VEGF-D cDNA into ischemic myocardium. However, presence replication-competent vectors adenovirus products widely recognized problem that may pose potential safety risk to treated patients. We compared three different GT production lots containing levels (RCA)...
Diseases caused by obstruction or rupture of vulnerable plaques in the arterial walls such as cardiovascular infarction stroke are leading cause death world. In present work, we developed human serum albumin nanoparticles loaded physisorption with zinc phthalocyanine, TT1, mainly used for industrial application near-infrared photosensitizer and compared these to HSA NPs well-known silicone phthalocyanine (Pc4). The use NIR light allows better tissue penetration, while permits high local...