A5076487947- Renal and related cancers
- Protist diversity and phylogeny
- Microbial Community Ecology and Physiology
- Renal cell carcinoma treatment
- Organ Donation and Transplantation
- Urological Disorders and Treatments
- Gold and Silver Nanoparticles Synthesis and Applications
- Laser-Ablation Synthesis of Nanoparticles
- Pluripotent Stem Cells Research
- Atherosclerosis and Cardiovascular Diseases
- Reproductive Biology and Fertility
- Pediatric Urology and Nephrology Studies
- Vibrio bacteria research studies
- Tissue Engineering and Regenerative Medicine
- Chromosomal and Genetic Variations
- Advanced biosensing and bioanalysis techniques
- RNA Research and Splicing
- 3D Printing in Biomedical Research
- Nonlinear Optical Materials Studies
- Extracellular vesicles in disease
- Optical Imaging and Spectroscopy Techniques
- Geochemistry and Elemental Analysis
- Barrier Structure and Function Studies
- Diamond and Carbon-based Materials Research
- Ureteral procedures and complications
University of Oulu
2015-2024
University of Eastern Finland
2023
Heidelberg University
2020
Institute of Applied Mathematics
2020
Indiana University
2020
Oulu University Hospital
2020
Borealis (Finland)
2019
Biocenter Finland
2016-2019
Institute of Cytology
1997-2009
University of Tübingen
2001-2007
Abstract Aims Vascular smooth muscle cells (SMCs) and their derivatives are key contributors to the development of atherosclerosis. However, studying changes in SMC gene expression heterogeneous vascular tissues is challenging due technical limitations high cost associated with current approaches. In this paper, we apply translating ribosome affinity purification sequencing profile SMC-specific directly from tissue. Methods results To facilitate translatome analysis, generated SMCTRAP mice,...
Kidney development depends crucially on proper ureteric bud branching giving rise to the entire collecting duct system. The transcription factor HNF1B is required for early steps of branching, yet molecular and cellular events regulated by are poorly understood. We report that specific removal Hnf1b from leads defective cell-cell contacts apicobasal polarity during events. High-resolution ex vivo imaging combined with a membranous fluorescent reporter strategy show decreased mutant cell...
The Müllerian duct (MD) is the anlage of oviduct, uterus and upper part vagina, main parts female reproductive tract. Several wingless-type mouse mammary tumor virus (MMTV) integration site family member (Wnt) genes, including Wnt4, Wnt5a Wnt7a, are involved in development MD its derivatives, with Wnt4 particularly critical, since fails to develop absence. We use, here, Wnt4EGFPCre-based fate mapping demonstrate that tip cells subsequent derived from Wnt4+ lineage cells. Moreover, required...
The kidney vasculature is critical for renal function, but its developmental assembly mechanisms remain poorly understood and models studying dynamics are limited. Here, we tested whether the embryonic contains endothelial cells (ECs) that heterogeneous with respect to VEGFR2/Flk1/KDR, CD31/PECAM, CD146/MCAM markers. Tie1Cre;R26RYFP-based fate mapping a time-lapse in organ culture successfully depicted of development correlation process CD31+ EC network. Depletion Tie1+ or ECs from kidneys,...
The embryonic mammalian metanephric mesenchyme (MM) is a unique tissue because it competent to generate the nephrons in response Wnt signaling. An ex vivo culture which MM separated from ureteric bud (UB), natural inducer, can be used as classic tubule induction model for studying nephrogenesis. However, technological restrictions currently prevent using this study molecular genetic details before or during induction. Using nephron segment-specific markers, we now show that also leads...
The epithelial ureteric bud is critical for mammalian kidney development as it generates the ureter and collecting duct system that induces nephrogenesis in dicrete locations mesenchyme during its emergence. We show a secreted Bmp antagonist Cerberus homologue (Cer1) fine tunes organization of tree organogenesis mouse embryo. Both enhanced expression Cer1 knock out enlarge size, these changes are associated with an altered three-dimensional structure revealed by optical projection...
Abstract Efficient delivery of genetic material to primary cells remains challenging. Here, efficient transfer is presented using synthetic biodegradable nanocarriers, resembling extracellular vesicles in their biomechanical properties. This based on two main technological achievements: generation soft polyelectrolyte capsules nanosize and application the nanocapsules for co‐transfer different RNAs tumor cell lines cells, including hematopoietic progenitor T cells. Near 100% efficiency...
Three-dimensional (3D) bioprinting is an evolving technique that expected to revolutionize the field of regenerative medicine. Since organ donation does not meet demands for transplantable organs, it important think another solution, which may and most likely will be provided by technology 3D bioprinting. However, even smaller parts printed renal tissue help, e.g. in developing better drugs. Some simple tissues such as cartilage have been with success, but a lot work still required...
Plasmon-resonant nanostars (NSts) have recently found applications in biophotonics due to their unique optical and chemical characteristics, showing comparable or superior properties than other anisotropic plasmon-resonant nanoparticles. In this paper, we synthesized gold NSts by the seed-mediated surfactant-free method. By varying diameters amount of spherical seeds tuned final tip-to-tip sizes 50, 82, 100, 120 nm ensuring peak location between 710 830 nm, scattering/absorption ratio at...
Further developments of antibacterial coatings based on photocatalytic nanomaterials could be a promising route towards potential environmentally friendly applications in households, public buildings and health care facilities. Hereby we describe simple chemical approach to synthesize nanomaterial-embedded using gypsum as binder. Various types TiO2 nanofiber-based materials (nitrogen-doped and/or palladium nanoparticle decorated) their composites with were characterized by means scanning...
Tissue, organ and organoid cultures provide suitable models for developmental studies, but our understanding of how the organs are assembled at single cell level still remains unclear. We describe here a novel Fixed Z-Dimension (FiZD) culture setup that permits high-resolution confocal imaging organoids embryonic tissues. In FiZD permeable membrane compresses tissues onto glass coverslip spacers adjust thickness, enabling tissue to grow up 12 days. Thus kidney rudiment can limited...
Laser optoporation of cells by local heating plasmonic gold nanoparticles (GNPs) was proven as a favorable delivery method molecules into cells. The efficiency depends on the laser beam intensity and GNP properties. Here, we evaluate membrane in vitro terms fluorescent dye permeability under treatment multi-pulsed nanosecond 1064 nm with sharply-focused beam. Anisotropic GNPs, such nanorods nanostars, were fabricated to achieve optimal GNP-cell interaction. Nanostars demonstrated highest...
In the framework of novel medical paradigm red blood cells (RBCs) have a great potential to be used as drug delivery carriers. This approach requires an ultimate understanding peculiarities mutual interaction RBC influenced by nano-materials composed drugs. Optical tweezers (OT) is widely explore mechanisms cells’ with ability trap non-invasively, manipulate and displace living notably high accuracy. current study, polymeric nano-capsules (NCs) investigated utilizing two-channel OT system....
In this work, several optical-spectroscopic methods have been used to visualize and investigate the penetration of diamond nanoparticles (NPs) various sizes (3-150 nm), surface structures fluorescence properties into animal skin in vitro. Murine samples treated with nanodiamond (ND) water suspensions studied using optical coherence tomography (OCT), confocal two-photon microscopy lifetime imaging (FLIM). An analysis nanodiamonds (NDs) enables selection optimal or their combination for study...
Generation of kidney organoids from pluripotent stem cells (PSCs) is regarded as a potentially powerful way to study development, disease, and regeneration. Direct differentiation PSCs towards renal lineages well studied; however, most the studies relate generation nephron progenitor population PSCs. Until now, into ureteric bud (UB) has had limited success. Here, we describe simple, efficient, reproducible protocol direct mouse embryonic (mESCs) UB cells. The mESC-derived were able induce...
Kidney diseases such as glomerulopathy and nephron dysfunction are estimated to grow more than 900 million cases by 2030, in 45% of which kidney transplantation will be required, representing a major challenge for biomedicine. A wealth progress has been made model human using induced pluripotent stem cells (iPSCs) vitro differentiated variety organoids, including developing various microfluidics-based organ-on-a-chip (OoC) systems based on them. With the combination targeted gene editing...
ABSTRACT Infection experiments were performed incubating Paramecium caudatum with non‐infectious free‐living bacteria or weakly infectious intracellular together the Holospora obtusa . Two of four ( Enterobacter aerogenes and Klebsiella pneumoniae ) found to get into nuclei when added H. The endonuclear bacterium Nonospora macronucleata that is by itself increases its infectivity presented holosporas. results provide evidence may facilitate entry other,
Three-dimensional organoids provide a new way to model various diseases, including cancer. We made use of recently developed kidney organ primordia tissue engineering technologies create novel renal for cancer gene discovery. then tested whether our assays can be used examine development. First we identified the transcriptome profiles quiescent embryonic metanephric mesenchymes (MM) and MM in which nephrogenesis program had been induced ex vivo. The were compared RCC patients controls....
Introduction: Urinary stents have been around for the last 4 decades, urinary catheters even longer. They are associated with infections, encrustation, migration, and patient discomfort. Research efforts to improve them shifted onto molecular cellular levels. ENIUS brought together translational scientists implants reduce morbidity.Methods & materials: A working group within network was tasked assessing future research lines improvement of implants.Topics were researched systematically using...
Abstract The organization and molecular composition of complicated Cajal bodies (CBs) interchromatin granule clusters (IGCs) in oocytes the house cricket, Acheta domesticus , were studied using immunofluorescent/confocal Immunogold labeling/electron microscopy. In A. oocytes, CB consists fibrillar matrix a central cavity containing predominantly granular body with insertions tightly packed material. latter structure was identified as an “internal” IGC, since it is enriched SC35 protein,...