A5043207861- Advanced biosensing and bioanalysis techniques
- Pesticide Exposure and Toxicity
- Pesticide and Herbicide Environmental Studies
- Carbon and Quantum Dots Applications
- Biosensors and Analytical Detection
- Electrochemical sensors and biosensors
- Environmental Toxicology and Ecotoxicology
- Allelopathy and phytotoxic interactions
- Stress Responses and Cortisol
- Nanocluster Synthesis and Applications
- SARS-CoV-2 detection and testing
- Analytical chemistry methods development
- Air Quality and Health Impacts
- Computational Drug Discovery Methods
- Paraquat toxicity studies and treatments
- Insect and Pesticide Research
- Pregnancy and Medication Impact
- Climate Change and Health Impacts
- Molecular Sensors and Ion Detection
- Air Quality Monitoring and Forecasting
- Analytical Chemistry and Chromatography
- Indoor Air Quality and Microbial Exposure
- Carcinogens and Genotoxicity Assessment
- Cholinesterase and Neurodegenerative Diseases
- DNA and Nucleic Acid Chemistry
Mahidol University
2020-2024
Oregon State University
2015-2019
Corvallis Environmental Center
2016-2017
Kasetsart University
2006
We have developed a simple and direct method to fabricate paper-based microfluidic devices that can be used for wide range of colorimetric assay applications. With these devices, assays performed within minutes allow quantitative analysis by use widely accessible iPhone camera an RGB color reader application (app) measure intensity. In the described laboratory experiment, students design create their own with common supplies such as Kimwipes, Parafilm, thermal laminator, gain hands-on...
A sensitive, selective, environmentally friendly, high-throughput, well-plate-based immunosorbent assay was developed to detect human α-fetoprotein (AFP) using carbon dots (C-Dots). Highly fluorescent C-Dots were synthesized a one-step hydrothermal reaction, with citric acid serving as the source and ethylene diamine acting nitrogen source. The reaction conditions optimized obtain desired surface functionality. Then, used label one member of anti-AFP pair (Ab2) via amine-amine coupling...
Non-toxic, fluorescent carbon nanodot labels are employed as novel ratiometric immunosensors for α-fetoprotein (AFP), a liver cancer biomarker. The assay generates broad linear range, low detection limit, and can be adapted to variety of immunoassay targets.
The objective of this study was to investigate the relationship acute pesticide exposures and changes in thyroid hormones among Thai farmers. We recruited 78 farmers, who were scheduled spray insecticides (chlorpyrifos and/or cypermethrin) or herbicides (paraquat glyphosate). On day before spraying, farmers collected their first morning void urine went for blood collection. day, samples at end spraying event they interviewed with questionnaires. next morning, collected. Blood analyzed...
A detection method for type-II pyrethroids in an environmental water sample using a microfluidic paper-based analytical device (µPAD) is reported here. The approach based on the formation of cyanide from hydrolysis and colorimetric layer-based µPAD. Parafilm inexpensive laminating pouches were used to create hydrophobic barrier assay This was selective which tested. calibration curves cypermethrin, deltamethrin, cyhalothrin, fenvalerate ranged 2 40 µg/mL without preconcentration. lower...
Described is a new approach to building microfluidic quantum dot wavelength-ratiometric sensor system for quantifying copper in water and biological samples.
A novel approach to building a membrane-based disposable well-plate, here applied cyanide detection, is described. Chitosan encapsulated CdTe quantum dots with maximum emission at 520 nm (CS-QD520) were used as fluorophores. The CS-QD520 nanoparticle was specifically quenched by copper(II), and the (Cu-CS-QD520) deposited onto glass microfiber filter (GF/B). Subsequent introduction of ion resulted in fluorescence recovery. "signal-ON" linearly correlated concentrations range 38.7 200 μM...
Chemical synthesis of oligonucleotides is a widely used tool in the field biochemistry. Several methods for gene have been introduced growing area genomics. In this paper, novel method constructing dsDNA proposed. Short (28-mer) oligo fragments from library were assembled through successive annealing and ligation processes, followed by PCR. First, two annealed to form molecule. The double-stranded was immobilized onto magnetic beads (solid support) via streptavidin-biotin binding. Next,...
Organophosphate (OP) pesticides are used by most farmers to remove insects and increase productivity; however, questions remain on the long-term health impacts of their use. This study assessed relationship between OP biomarker levels metabolic parameters. Conventional (n = 213) organic 225) were recruited, interviewed, had physical examinations. Serum glucose lipid profiles, triglycerides, total cholesterol, high-density lipoproteins (HDL), low-density (LDL) measured. The average age,...
Cypermethrin, a pyrethroid insecticide, is frequently spread on agricultural farmlands and also used in households Thailand. Conventional pesticide-using farmers (n = 209) were recruited from the Phitsanulok Nakornsawan provinces. Certified organic 224) Yasothorn province. The interviewed via questionnaire urine their first morning void was collected. samples analyzed for 3-phenoxybenzoic acid (3-PBA), cis-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropane carboxylic (cis-DCCA),...
Alteration of the hypothalamic-pituitary-adrenal (HPA) axis hormones has been associated with a range chronic metabolic and cardiovascular health conditions. This study evaluated whether type farming (organic versus conventional) or number self-reported days spraying pesticides in past 8 months was diurnal cortisol levels. Salivary levels were measured four times day (waking, 30 min after waking, 6 h waking bedtime) longitudinally, apart during three rounds data collection. Pesticide using...
Background : Organophosphates and carbamates exposure is typically gauged by serum Butyrylcholinesterase (BChE) activity decline, reflecting their enzyme-inhibiting effects. This research aimed to compare differences in BChE health conditions between elderly conventional organic farmers.
Purpose The purpose of this study was to develop an accurate, selective, low-cost and user-friendly colorimetric pad detect formaldehyde at low concentration. Design/methodology/approach 1-phenyl-1,3-butanedione, a reactive chemical, selected the for indoor air measurement. Silica nanoparticle impregnated with chemical coated on cellulose filter surface increase site. A certified permeation tube used generate six varied concentrations between 0.01 0.10 ppm in test chamber. color intensity...
Acetochlor is a chloroacetanilide selective pre-emergent herbicide used for controlling grass and broadleaf weeds in crops. This study compared the acetochlor exposures of backpack tractor sprayers assessed whether dermal or air were more important contributors to overall body burden as measured by urinary metabolites. Sixty sugarcane farmers Nakhonsawan province, Thailand participated study, breathing zone patch samples collected during spraying. Urine before spraying, at end spraying task,...