A5034145117- Biosensors and Analytical Detection
- HIV/AIDS drug development and treatment
- Cancer therapeutics and mechanisms
- Innovative Microfluidic and Catalytic Techniques Innovation
- Microfluidic and Capillary Electrophoresis Applications
- RNA Interference and Gene Delivery
- COVID-19 Clinical Research Studies
- Single-cell and spatial transcriptomics
- Regulation of Appetite and Obesity
- Extracellular vesicles in disease
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Escherichia coli research studies
- Dietary Effects on Health
- Computational Drug Discovery Methods
- Tuberculosis Research and Epidemiology
- Viral gastroenteritis research and epidemiology
- Circadian rhythm and melatonin
- Child Nutrition and Feeding Issues
- Psoriasis: Treatment and Pathogenesis
- Cytokine Signaling Pathways and Interactions
- Occupational and environmental lung diseases
- Occupational exposure and asthma
- Synthesis and Characterization of Heterocyclic Compounds
- Immunotherapy and Immune Responses
- Immune responses and vaccinations
Tulane University
2022-2024
National Institute of Allergy and Infectious Diseases
2020
National Institutes of Health
2020
University of Virginia
2016-2020
Vector Oncology (United States)
2020
Division of Chemistry
2016
Charlottesville Medical Research
2016
Oregon State University
2015
We have developed a simple and direct method to fabricate paper-based microfluidic devices that can be used for wide range of colorimetric assay applications. With these devices, assays performed within minutes allow quantitative analysis by use widely accessible iPhone camera an RGB color reader application (app) measure intensity. In the described laboratory experiment, students design create their own with common supplies such as Kimwipes, Parafilm, thermal laminator, gain hands-on...
Current colorimetric presumptive identification of illicit drugs for determining illegal possession controlled substances by law enforcement relies solely on the subjective interpretation color change using drug- or class-specific reactions. Here, we describe use inexpensive polyester-toner, rotation-driven microfluidic devices with a smartphone as potential alternative current field-testing drugs, allowing an objective and user-friendly image analysis technique detection. The centrifugal...
Abstract Lung tissue resident memory (TRM) cells are thought to play crucial roles in lung host defense. We have recently shown that immunization with the adjuvant LTA1 (derived from A1 domain of E. coli heat labile toxin) admixed OmpX K. pneumoniae can elicit antigen specific Th17 TRM provide serotype independent immunity members Enterobacteriaceae family. However, upstream requirements generate these unclear. Single-cell RNA-seq showed vaccine-elicited expressed high levels IL-1R1,...
Networks of neurons control feeding and activity patterns by integrating internal metabolic signals energy balance with external environmental cues such as time-of-day. Proper circadian alignment behavior is necessary to prevent disease, thus it imperative that molecular players maintain neuronal coordination homeostasis are identified. Here, we demonstrate mice lacking the p75 neurotrophin receptor, p75NTR, decrease their food anticipatory (FAA) in response daytime, but not nighttime,...
The use of overhead transparencies as substrates for the fabrication microfluidic devices brings new capabilities in creation fully-integrated systems.
WR99210, a former antimalarial drug candidate now widely used for the selection of Plasmodium transfectants, selectively targets parasite’s dihydrofolate reductase thymidine synthase bifunctional enzyme (DHFR-TS) but not human DHFR, which is fused with TS. Accordingly, WR99210 and plasmids expressing dhfr gene have become valued tools genetic modification parasites in laboratory. Concerns over ineffectiveness from some sources encouraged us to investigate biological chemical differences...
ABSTRACT WR99210, a former antimalarial drug candidate now widely used for the selection of Plasmodium transfectants, selectively targets parasite dihydrofolate reductase thymidine synthase bifunctional enzyme (DHFR-TS) but not human DHFR, which is fused with TS. Accordingly, WR99210 and plasmids expressing dhfr have become valued tools genetic modification parasites in laboratory. Concerns over ineffectiveness from some sources encouraged us to investigate biological chemical differences...