A5016661296- Caveolin-1 and cellular processes
- Metabolism, Diabetes, and Cancer
- RNA Research and Splicing
- Nuclear Structure and Function
- Lipid metabolism and biosynthesis
- Pancreatic function and diabetes
- Ion Transport and Channel Regulation
- Glycogen Storage Diseases and Myoclonus
- Protein Kinase Regulation and GTPase Signaling
- Signaling Pathways in Disease
- Erythrocyte Function and Pathophysiology
- Protist diversity and phylogeny
- Carbohydrate Chemistry and Synthesis
- Legume Nitrogen Fixing Symbiosis
- Lipid metabolism and disorders
- Hemoglobin structure and function
- Cancer Cells and Metastasis
- Effects of Radiation Exposure
- Phytase and its Applications
- TGF-β signaling in diseases
- Soybean genetics and cultivation
- Diet, Metabolism, and Disease
- Plant Toxicity and Pharmacological Properties
- Plant responses to water stress
- Phytoestrogen effects and research
Gyeongsang National University
2013-2024
Roswell Park Comprehensive Cancer Center
2006-2007
University of Virginia
1992-2005
The University of Texas at Austin
1990-1991
We report here the in vivo conversion of [3H]myoinositol to [3H]chiroinositol. After labeling intraperitoneally with for 3 days reach radioisotope equilibrium urine, [3H]chiroinositol was isolated from tissues and purified after 6 N HCl hydrolysis by two sequential paper chromatographies high performance liquid chromatography (HPLC). Percent highest urine (36%), liver (8.8%), muscle blood (7.6%) intestine, brain, kidney, spleen, heart decreasing percentage 2.8 0.7%. Labeling other inositol...
Flotillin-1 (Flot-1) has been shown to regulate cancer progression, but the regulatory role of post-translational modifications Flot-1 on cancers remains elusive. Herein, we show that up-regulated E2 conjugating enzyme UBC9 sumoylates at Lys-51 and Lys-195 with small ubiquitin-like modifier (SUMO)-2/3 modification in metastatic prostate cancer. Mitogen induced sumoylation nuclear translocation Flot-1. The nuclear-targeted physically interacted Snail, inhibited Snail degradation through...
Here, we explored flotillin-1-mediated regulation of insulin-like growth factor-1 (IGF-1) signaling. Flotillin-1-deficient cells exhibited a reduction in the activation IGF-1 receptor (IGF-1R), ERK1/2 and Akt pathways, transcriptional Elk-1 proliferation response to were reduced these cells. We found that IGF-1-independent flotillin-1 palmitoylation at Cys34 endoplasmic reticulum (ER) was required for ER exit plasma membrane localization IGF-1R. IGF-1-dependent depalmitoylation...
Adherence of Entamoeba histolytica trophozoites to colonic mucins and resistance lysis by the membrane attack complex complement are mediated a galactose- N-acetyl-D-galactosamine-specific cell-surface lectin. This lectin is heterodimeric glycoprotein heavy (170 kDa) light (35/31 subunits. In this work, amino acid sequence anchor subunit were analyzed. The cDNA encoded protein with calculated molecular mass 32 kDa containing two potential sites for N-linked glycosylation putative amino-...
We investigated the effect of phenylephrine (PE)- and isoproterenol (ISO)-induced cardiac hypertrophy on subcellular localization expression caveolin-3 STAT3 in H9c2 cardiomyoblast cells. Caveolin-3 to plasma membrane was attenuated caveolae 24.3% reduced by catecholamine-induced hypertrophy. phospho-STAT3 were up-regulated but verapamil cyclosporin A synergistically decreased levels PE- ISO-induced hypertrophic Both activation increased nucleus Immunofluorescence analysis revealed that...
// Hee Jun Cho 1,* , Sun-Mi Park In-Kyu Kim In-Koo Nam Kyoung Eun Baek 1 Min-Ju Im Jong-Min Yoo Seung-Ho Ki-Jun Ryu Hyun-Tak Han Hyo-Jin Soon-Chan Hong 2 Kwang Dong Yunbae Pak Jae Won Chang Lee and Jiyun Division of Applied Life Science (BK21 plus), Research Institute Sciences, Gyeongsang National University, Jinju, Korea Department Surgery, School Medicine, * These authors contributed equally to the work Correspondence: Yoo, email: Keywords : RhoGDI2, EMT, Snail, gastric cancer, invasion...
HIRc-B cells (rat fibroblasts expressing the human insulin receptor) were incubated with myo-[3H]inositol for 48 hr, and biosynthesis of chiro-[3H]inositol was investigated in absence presence following a time course up to 60 min. After phase separation, treatment 15 min caused 2.2-fold increase specific radioactivity chiro-[3H]inositol-containing phospholipids contrast 1.2-fold myo-[3H]inositol-containing phospholipids. No insulin-mediated change observed inositol phosphates or free...
We investigated whether altering caveolin-2 (cav-2) expression affects the proliferation of cancer cells. Cav-2 was not detected in HepG2, SH-SY5Y and LN-CaP cells, loss cav-2 restored by 5-aza-2'-deoxycytidine treatment. In contrast, C6, HeLa, A549, MCF7 PC3M cells expressed cav-2. Effects re-expression exogenous siRNA-mediated down-regulation endogenous on were examined MTT assay, colony formation assay flow cytometric analysis. transfection HepG2 hepatocellular carcinoma knockdown C6...
Insulin controls transcription to sustain its physiologic effects for the organism adapt environmental changes added genetic predisposition. Nevertheless, insulin-induced transcriptional regulation by epigenetic factors and in defined nuclear territory remains elusive. Here we show that inner membrane (INM)-integrated caveolin-2 (Cav-2) regulates insulin-response activation of Egr-1 JunB genes at periphery. INM-targeted pY19-Cav-2 response insulin associates specifically with A-type lamin,...
Abstract Here, we identify that Caveolin‐2 (Cav‐2), an integral membrane protein, controls adipocyte hypertrophy in association with nuclear lamina. In the stage of adipogenesis, pY19‐Cav‐2 lamin A/C facilitated disengagement CCAAT/enhancer‐binding protein α (C/EBPα) and peroxisome proliferator‐activated receptor γ (PPARγ) from repressed Cav‐2 promoter at periphery for epigenetic activation , thereby promoted C/EBPα PPARγ‐induced hypertrophy. Stable expression was required retained by...
Mitogenic regulation by caveolin-2 in response to insulin was investigated. Insulin triggered phosphorylation of on tyrosine 19. increased the interaction between pY19-caveolin-2 and phospho-ERK, that inhibited a MEK inhibitor U0126. Insulin-induced with phospho-ERK prevented when 19 is mutated alanine. relocalized nucleus their nuclear co-localization impaired Down-regulation siRNA arrested insulin-induced localization ERK no change insulin-stimulated activation. Of consequence, attenuated...
Herein, we report that insulin-activated extracellular signal-regulated kinase (ERK) is translocated to the nuclear envelope by caveolin-2 (cav-2) and associates with lamin A/C in inner membrane response insulin. We identified Ser154–Val155–Ser156 domain on C-terminal of cav-2 essential for insulin-induced phosphorylation targeting ERK cav-2. In human embryonic kidney 293T cells, was not activated nucleus insulin comparison insulin-like growth factor-1 (IGF-1). However, insulin-stimulated...
We report our comparative studies of myo- and chiro [3H]inositol distribution in control nondiabetic Wistar spontaneous nonobese insulin-resistant Type II diabetic G.K. rat tissues following 78-h labeling period. From various tissue extracts, free inositols, inositol phosphates, phospholipids were isolated analyzed. Our findings demonstrate (1) a significant difference the metabolism chiro-inositol normal rats, (2) severe defect conversion myo-[3H]inositol to chiro-[3H]inositol at...
We investigated glucose uptake and the translocation of Akt caveolin-3 in response to insulin H9c2 cardiomyoblasts exposed an experimental resistance condition 100 nM a 25 mM containing media for 24 h. The cells under exhibited decrease insulin-stimulated 2-deoxy[3 H]glucose as compared control grown 5 media. In addition reduction insulin-induced membranes, we observed significant membrane association phosphorylated with consequent increase cytosolic pool. Actin remodeling was also greatly...
Here, we have identified a retrograde transport pathway of caveolin-2 (cav-2) for its regulatory function in the nucleus. Confocal microscopy analysis, photoactivation experiments and subcellular fractionation revealed that cav-2 localized Golgi was transported to inner nuclear membrane (INM) response insulin. Exogenous caveolin-1 (cav-1) P132L-cav-1 expression did not affect localization insulin-induced INM targeting cav-2. Cav-2(DKV) mutant endoplasmic reticulum (ER) unable translocate The...