A5030331320- Salmonella and Campylobacter epidemiology
- Viral gastroenteritis research and epidemiology
- Escherichia coli research studies
- Bacterial Infections and Vaccines
- Blood Coagulation and Thrombosis Mechanisms
- Inflammasome and immune disorders
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Infective Endocarditis Diagnosis and Management
- Pneumonia and Respiratory Infections
- Bacteriophages and microbial interactions
- Microscopic Colitis
- Clostridium difficile and Clostridium perfringens research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- COVID-19 Clinical Research Studies
- Immune Response and Inflammation
- Hepatitis Viruses Studies and Epidemiology
University of Birmingham
2020-2025
Institute of Immunology
2024
Public Health England
2018-2020
Despite high vaccination coverage, Bordetella pertussis the causative agent of whooping cough is still a health concern worldwide. A resurgence cases has been reported, particularly in countries using acellular vaccines with waning immunity and pathogen adaptation thought to be responsible. better understanding protective immune responses needed for development improved vaccines. In our study, B. strain B1917 variants presenting single gene deletion were generated analyze role vaccine...
Abstract Inflammation-induced thrombosis is a common consequence of bacterial infections, such as those caused by Salmonella Typhimurium (STm). The presentation multi-organ post-infection that develops and resolves with organ-specific kinetics raises significant challenges for its therapeutic control. Here, we identify specific inflammatory events driving in the spleens livers STm-infected mice. IFN-γ or platelet expression C-type lectin-like receptor CLEC-2, key drivers liver, are...
Introduction Vaccination with Vi capsular polysaccharide (Vi-PS) or protein-Vi typhoid conjugate vaccine (TCV) can protect adults against Salmonella Typhi infections. TCVs offer better protection than Vi-PS in infants and may adults. Potential reasons for why TCV be superior are not fully understood. Methods results Here, we immunized wild-type (WT) mice deficient IgG IgM (Vi conjugated to tetanus toxoid CRM197) up seven months, without subsequent challenge Vi-expressing Typhimurium....
Whooping cough is a re-emerging respiratory tract infection. It has become clear that there need for better understanding of protective immune responses and variation between Bordetella pertussis strains to aid the development improved vaccines. In order survive in host, B. evolved mechanisms evade complement-mediated killing, including ability bind complement-regulatory proteins. Here we evaluate interactions with complement system among recently isolated strains. Isolates whose genomes...
Germinal centers (GCs) are sites where plasma and memory B cells form to generate high-affinity, Ig class-switched antibodies. Specialized stromal called follicular dendritic (FDCs) essential for GC formation. During systemic Salmonella Typhimurium (STm) infection GCs absent, whereas extensive extrafollicular switched antibody responses maintained. The mechanisms that underpin the absence of formation incompletely understood. Here, we demonstrate STm induces a reversible disruption niches...
Mucosal vaccination aims to prevent infection mainly by inducing secretory IgA (sIgA) antibody, which neutralises pathogens and enterotoxins blocking their attachment epithelial cells. We previously demonstrated that encapsulated protein antigen CD0873 given orally hamsters induces neutralising antibodies locally as well systemically, affording partial protection against Clostridioides difficile infection. The aim of this study was determine whether displaying on liposomes, mimicking native...
Clearance of intracellular infections caused by Salmonella Typhimurium (STm) requires IFN-γ and the Th1-associated transcription factor T-bet. Nevertheless, whereas IFN-γ-/- mice succumb rapidly to STm infections, T-bet-/- do not. In this study, we assess anatomy immune responses relationship with bacterial localization in spleens livers STm-infected mice. mice, there is deficient granuloma formation inducible NO synthase (iNOS) induction, increased dissemination bacteria throughout organs,...
Abstract Inflammation-induced thrombosis is a common consequence of bacterial and viral infections, such as those caused by Salmonella Typhimurium (STm) SARS-CoV-2. The identification multi-organ the chronological differences in its induction resolution raises significant challenges for successfully targeting infection-associated thrombosis. Here, we identified specific pathways effector cells driving spleen liver following STm infection. Thrombosis independent IFN-γ or platelet C-type...
Lipopolysaccharide O-antigen is an immunodominant target of protective antibodies. Variation in structures limits antibody-mediated cross-protection between closely-related pathogens including Salmonella Typhimurium (STm) and S . Enteritidis (SEn). Bacterial outer membrane vesicles (OMV) are vaccine platforms presenting surface antigens their natural conformations. To assess how lengths impact antibody responses control homologous or heterologous infection, mice were immunized with STm-OMV...
Abstract Vaccination with Vi capsular polysaccharide (Vi-PS) or protein-Vi typhoid conjugate vaccine (TCV) can protect adults against Salmonella Typhi infections. TCVs offer better protection than Vi-PS in infants and may adults. Potential reasons for why TCV be superior are not fully understood. Here, we immunized wild-type (WT) mice deficient IgG IgM (Vi conjugated to tetanus toxoid CRM 197 ) up seven months, without subsequent challenge Vi-expressing Typhimurium. Unexpectedly, alone were...
Summary Germinal centres (GCs) are sites where plasma and memory B cells form to generate high-affinity, Ig class switched antibodies. Specialised stromal called follicular dendritic (FDCs) essential for GC formation. During systemic Salmonella Typhimurium (STm) infection GCs absent, whereas extensive extrafollicular antibody responses maintained. The mechanisms that underpin the absence of formation incompletely understood. Here, we show STm-induces a reversible disruption niches within...