A5065709499- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Fungal Diseases
- Fungal and yeast genetics research
- Fungal Plant Pathogen Control
- Plant Disease Resistance and Genetics
- Mycotoxins in Agriculture and Food
- Autophagy in Disease and Therapy
- Fungal Biology and Applications
- Yeasts and Rust Fungi Studies
- Metabolomics and Mass Spectrometry Studies
- Chromatography in Natural Products
- Insect-Plant Interactions and Control
- Phytoplasmas and Hemiptera pathogens
- Plant Virus Research Studies
- Diet, Metabolism, and Disease
- Insect Resistance and Genetics
- Bioactive natural compounds
- Sirtuins and Resveratrol in Medicine
- Traditional Chinese Medicine Analysis
- Plant Gene Expression Analysis
- Cancer Treatment and Pharmacology
- Biochemical Analysis and Sensing Techniques
- Probiotics and Fermented Foods
- 14-3-3 protein interactions
- Botany and Plant Ecology Studies
Heilongjiang University of Chinese Medicine
2024-2025
Qingdao Agricultural University
2020-2024
Nanjing Agricultural University
2014-2020
China Pharmaceutical University
2007-2008
Botrytis cinerea is a filamentous phytopathogen with high risk of developing resistance to fungicides. The phenylpyrrole fungicide fludioxonil has been reported have excellent activity against B. and increasingly applied control gray mold in China. In this study, molecular biochemical characteristics laboratory field mutants resistant investigated. During 2012 2014, isolates collected from Jiangsu Shandong Provinces China were tested vitro for sensitivity fungicides commonly used suppress...
Abstract A simple, accurate, precise, specific and reproducible high‐performance liquid chromatography (HPLC) method was developed for simultaneous determination of resveratrol isomers in rat plasma. Cis ‐resveratrol made by exposure a trans solution to sunlight 5 days followed separation HPLC identification mass spectrometry (MS). The assay procedure involved simple liquid–liquid extraction internal standard (IS, caffeine) from small plasma volume directly into acetonitrile. supernatant...
Autophagy, a ubiquitous intracellular degradation process, is conserved from yeasts to humans. It serves as major survival function during nutrient depletion stress and crucial for correct growth differentiation. In this study, we characterized an atg1 orthologue Bcatg1 in the necrotrophic plant pathogen Botrytis cinerea. Quantitative real-time polymerase chain reaction (qRT-PCR) assays showed that expression of BcATG1 was up-regulated under carbon or nitrogen starvation conditions. could...
Autophagy is a conserved degradation process that maintains intracellular homeostasis to ensure normal cell differentiation and development in eukaryotes. ATG8 one of the key molecular components autophagy pathway. In this study, we identified characterized BcATG8, homologue Saccharomyces cerevisiae (yeast) necrotrophic plant pathogen Botrytis cinerea Yeast complementation experiments demonstrated BcATG8 can functionally complement defects yeast null mutant. Direct physical interaction...
Glomerella leaf spot (GLS) caused by cingulata (Colletotrichum spp.) is a devastating disease of apple (Malus × domestica Borkh.) in China. We evaluated the effects temperature, moisture, and wetness duration on production conidia ascospores diseased leaves. The temperature required for producing G. leaves ranged from 20 to 30°C, with an optimum approximately 28°C. pathogen produced only when were wet or at RH=100%. Conidia could not form until maintained least 24 hours, modified Weibull...
High-osmolarity glycerol (HOG) signaling pathway belongs to mitogen-activated protein kinase (MAPK) cascades that regulate responses of organism diverse extracellular stimuli. The membrane spanning proteins Sho1 and Sln1 serve as biosensors HOG in Saccharomyces cerevisiae. In this study, we investigated the biological functions BcSHO1 BcSLN1 gray mold fungus Botrytis cinerea. Target gene deletion demonstrated both are important for mycelial growth, conidiation sclerotial formation. double...
The Basic Leucine Zipper (bZIP) transcription factors (TFs) family is among of the largest and most diverse gene families found in plant species, members bZIP TFs perform important functions developmental processes stress response. To date, genes Platycodon grandiflorus have not been characterized. In this work, a number 47 PgbZIP were identified from genome P. , divided into 11 subfamilies. distribution these on chromosome replication events analyzed. motif, structure, cis -elements,...
Nutrient and stress factor 1 (Nsf1), a transcription containing the classical Cys2-His2 (C2H2) zinc finger motif, is expressed under non-fermentable carbon conditions in response to salt Saccharomyces cerevisiae. However, role of Nsf1 filamentous fungi not well understood. In this study, orthologue was investigated Fusarium graminearum (named FgNsf1), causal agent head blight (FHB). The functions FgNsf1 were evaluated by constructing FgNSF1 deletion mutant, designated as ΔFgNsf1, its...
Fusarium wilt caused by oxysporum f. sp. melonis (FOM) is one of the most notorious seed-borne diseases worldwide. Phenamacril a cyanoacrylate fungicide with novel chemical structure and strong inhibitive activity against FOM. To evaluate risk FOM developing phenamacril resistance, five phenamacril-resistant mutants > 800 μg ml− 1 minimum inhibitory concentration were obtained repeated exposure to in laboratory. Compared parental isolate, four showed enhanced biological fitness sporulation...
Protein phosphatase complex Nem1/Spo7 plays crucial roles in the regulation of various biological processes eukaryotes. However, its functions phytopathogenic fungi are not well understood. In this study, genome-wide transcriptional profiling analysis revealed that Nem1 was significantly upregulated during infection process Botryosphaeria dothidea, and we identified characterized substrate Pah1 (a phosphatidic acid phosphatase) B. dothidea. physically interacted with dephosphorylated to...
The Str2 gene encodes a cystathionine γ-synthase that is key enzyme in methionine (Met) biosynthesis Saccharomyces cerevisiae. Met plays critical role protein synthesis and diverse cellular processes both eukaryotes prokaryotes. In this study, we characterized the orthologue BcStr2 Botrytis cinerea. mutant was unable to grow on minimal medium (MM). addition, conidia of were germinate water-agar within 15 h incubation. Supplementation with 1 mm or 0.5 mg/mL homocysteine, but not cysteine...
The S-adenosyl-L-homocysteine hydrolase (Sah1) plays a crucial role in methylation and lipid metabolism yeast mammals, yet its function remains elusive filamentous fungi. In this study, we characterized Sah1 the phytopathogenic fungus F. graminearum by generating knockout knockout-complemented strains of FgSAH1. We found that FgSah1-GFP fusion protein was localized to cytoplasm, deletion FgSAH1 resulted defects vegetative growth, asexual sexual reproduction, stress responses, virulence,...
Botryosphaeria canker and fruit rot caused by the fungus dothidea is one of most destructive diseases apple worldwide. Autophagy an evolutionarily conserved self-degradation process that important for maintaining homeostasis to ensure cellular functionality. To date, role autophagy in B. not well elucidated. In this study, we identified characterized autophagy-related protein Atg1 dothidea. The BdAtg1 deletion mutant ΔBdAtg1 showed blockade phenotypic defects mycelial growth, conidiation,...
Abstract Grape white rot is a destructive fungal disease caused by the pathogenic fungus Coniella vitis . The sensitivity of C. to fluazinam was tested select an efficient fungicide control this disease. Furthermore, baseline populations established based on EC 50 62 strains collected from different regions in which grapevines are grown China. were extremely sensitive fluazinam. efficiency grape determined with detached grapevine leaves. Fluazinam highly effective controlling Moreover, risk...