A5001309321- Cellular transport and secretion
- Parkinson's Disease Mechanisms and Treatments
- Neuroscience and Neuropharmacology Research
- Autophagy in Disease and Therapy
- Neurological diseases and metabolism
- Mitochondrial Function and Pathology
- Nerve injury and regeneration
- Lipid Membrane Structure and Behavior
- COVID-19 and Mental Health
- COVID-19 Pandemic Impacts
- Retinal Development and Disorders
- Neurotransmitter Receptor Influence on Behavior
- Drug Solubulity and Delivery Systems
- Immunotherapy and Immune Responses
- Alzheimer's disease research and treatments
- Cannabis and Cannabinoid Research
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Psychological Well-being and Life Satisfaction
- Gastrointestinal motility and disorders
- Advanced Drug Delivery Systems
- RNA regulation and disease
- Genetic Neurodegenerative Diseases
- COVID-19 epidemiological studies
- Gut microbiota and health
Stanford University
2018-2023
Center for Drug Evaluation and Research
2020
United States Food and Drug Administration
2020
European Neuroscience Institute Göttingen
2012-2017
The synaptotagmins (syts) are a family of molecules that regulate membrane fusion. There 17 mammalian syt isoforms, most which expressed in the brain. However, little is known regarding subcellular location and function majority these syts neurons, largely due to lack isoform-specific antibodies. Here we generated pHluorin-syt constructs harboring luminal domain pH sensor, reports localization, organelles targeted, kinetics sites exocytosis endocytosis. Of interest, only syt-1 2 targeted...
Delivery of neurotrophins and neuropeptides via long-range trafficking dense core vesicles (DCVs) from the cell soma to nerve terminals is essential for synapse modulation circuit function. But mechanism by which transiting DCVs are captured at specific sites unknown. Here, we discovered that Synaptotagmin-4 (Syt4) regulates capture spatial distribution in hippocampal neurons. We found highly mobile undergo translocation but switch directions only distal ends axons, revealing a circular...
Dysregulated iron or Ca
Brain-derived neurotrophic factor (BDNF) is widely reported to enhance synaptic vesicle (SV) exocytosis and neurotransmitter release. But it still unclear whether BDNF enhances SV recycling at excitatory terminals only, or both inhibitory terminals. In the present study, in a direct comparison using cultured rat hippocampal neurons, we demonstrate that spontaneous activity-dependent release from terminals, but not treatment for 5 minutes 48 hours increased activity-induced...
The sorting of activated receptors into distinct endosomal compartments is essential to activate specific signaling cascades and cellular events including growth survival. However, the proteins involved in this are not well understood. We discovered a novel role EndophilinAs BDNF-TrkB late compartments. Mice lacking all three accumulate Rab7-positive endosomes. Moreover, differentially localized to, co-traffic with, tubulate, compartments: In response BDNF, EndophilinA2 recruited both early...
There is a lack of reliable molecular markers for Parkinson’s disease (PD) patients and at-risk individuals. The detection the pre-symptomatic population PD will empower more effective clinical intervention to delay or prevent onset. We have previously found that mitochondrial protein Miro1 resistant depolarization-induced degradation in fibroblasts from large number several Therefore, has potential molecularly label populations. In order determine whether could serve as marker risk PD, here...
Abstract Parkinson’s disease (PD) is a debilitating movement disorder, significantly afflicting the aging population. Efforts to develop an effective treatment have been challenged by lack of understanding pathological mechanisms underlying neurodegeneration. We shown that Miro1, outer mitochondrial membrane protein, situates at intersection complex genetic and functional network PD. Removing Miro1 from surface damaged mitochondria prerequisite for clearance via mitophagy. proteins PINK1,...
Along with the major impact on public health, COVID-19 outbreak has caused unprecedented concerns ranging from sudden loss of employment to mental stress and anxiety. We implemented a survey-based data collection platform characterize how pandemic affected socio-economic, physical health conditions individuals. focused three broad areas, namely, changes in social interaction during home confinement, economic their status. identified substantial increase virtual among individuals, which might...
Mutations in MAPT gene cause multiple neurological disorders, including frontal temporal lobar degeneration and parkinsonism. Increasing evidence indicates impaired mitochondrial homeostasis mitophagy patients disease models of pathogenic . Here, using patients’ fibroblasts as a model, we report that disease-causing mutations compromise early events mitophagy. By employing biochemical assays discover upon depolarization, the recruitment LRRK2 Parkin to mitochondria degradation outer membrane...
The purpose of this investigation was to prepare a gastroretentive drug delivery system Ofloxacin. Ofloxacin is fluoroquinolone antibacterial which acts by inhibiting the topoisomerase enzyme essential in reproduction bacterial DNA. It highly soluble acidic media and precipitates alkaline thereby losing its solubility. Hence, developed enhance bioavailability retaining it environment stomach. Different formulations were formulated using various concentrations hydroxy propyl methyl cellulose,...
Abstract Along with the major impact on public health, COVID-19 outbreak has caused unprecedented concerns ranging from sudden loss of employment to mental stress and anxiety. We implemented a survey-based data collection platform characterize how pandemic affected socio-economic, physical health conditions individuals. focused three broad areas, namely, changes in social interaction during home confinement, economic their status. identified substantial increase virtual among individuals,...
Summary Dysregulated iron or Ca 2+ homeostasis has been reported in Parkinson’s disease (PD) models. Here we discover a connection between these two metals at the mitochondria. Elevation of levels causes inward mitochondrial overflow, through an interaction Fe with Mitochondrial Calcium Uniporter. In PD neurons, accumulation-triggered influx across surface leads to spatially confined elevation outer membrane, which is subsequently sensed by Miro1, -binding protein. A Miro1 blood test...
Right now, inside all of our bodies there is a complex ecosystem made up bacteria. Often when we think bacteria, disease, but most bacteria are beneficial and needed for maintaining good health. Most these live in the gut. Beneficial contribute to development help combat harmful that might make us sick. Therefore, if bacterial gut out balance too many it lead certain intestinal diseases. We have studied how heme iron, type iron found blood red meat, disturbs community gut, ultimately affects...
Our brains create and store memories. Do you know how brain cells communicate with each other to memories? Each cell (also called a neuron) talks neurons in the brain. These release chemicals neurotransmitters that enable help storing memory. We were specifically interested understanding growth factor brain-derived neurotrophic (BDNF) facilitates communication between region, hippocampus, as memories are formed stored here. found BDNF interacts endocannabinoids, which components of...