A5004071141- Ovarian cancer diagnosis and treatment
- Estrogen and related hormone effects
- Cancer-related molecular mechanisms research
- Ferroptosis and cancer prognosis
- Genomics, phytochemicals, and oxidative stress
- NF-κB Signaling Pathways
- Prostate Cancer Treatment and Research
- Inflammatory mediators and NSAID effects
- Immune cells in cancer
- FOXO transcription factor regulation
- Ultrasound and Hyperthermia Applications
- interferon and immune responses
- Cancer Research and Treatments
- Genetics, Bioinformatics, and Biomedical Research
- Immunodeficiency and Autoimmune Disorders
- Genomics and Rare Diseases
- Cardiac, Anesthesia and Surgical Outcomes
- Reproductive Physiology in Livestock
- Effects of Radiation Exposure
- Curcumin's Biomedical Applications
- Reproductive System and Pregnancy
- Hormonal and reproductive studies
- Cystic Fibrosis Research Advances
- Adsorption and biosorption for pollutant removal
- HIV Research and Treatment
Baylor College of Medicine
2025
The University of Texas MD Anderson Cancer Center
2022-2023
Tulane University
2015-2022
Center for Genomic Science
2022
Jewish Hospital
2019-2020
Barnes-Jewish Hospital
2019-2020
Washington University in St. Louis
2018-2020
This collaborative study, led by the Clinical Genome Resource Severe Combined Immunodeficiency Disease Variant Curation Expert Panel (ClinGen SCID-VCEP), implemented and adapted American College of Medical Genetics Genomics/Association for Molecular Pathology (ACMG/AMP) guidelines interpreting germline variants in genes with established relationships to SCID. The effort focused on 7 most common SCID-related identified SCID newborn screening North America: ADA , DCLRE1C IL2RG IL7R JAK3 RAG1...
Prostate cancer (PCa) cells expressing full-length androgen receptor (AR-FL) are susceptible to deprivation therapy (ADT). However, outgrowth of castration-resistant prostate (CRPC) can occur due the expression constitutively active (ligand-independent) AR splice variants, particularly AR-V7. We previously demonstrated that sulforaphane (SFN), an isothiocyanate phytochemical, decrease AR-FL levels in PCa cell lines, LNCaP and C4-2B. Here, we examined efficacy SFN targeting both AR-V7 CRPC...
Chronic genitourinary inflammation results in Leukocytospermia (LCS), an elevated number of white blood cells (WBCs) semen, which, association with oxidative stress, may suppress sperm function, and manifest as male factor infertility. The current clinical diagnosis LCS employs manual enumeration WBCs requires complex staining laboratory skills or measurement inflammatory cytokines chemokines levels. Many patients idiopathic infertility are asymptomatic. In search better markers for LCS, we...
Aberrant activation of the NF-κB transcription factors underlies chemoresistance in various cancer types, including colorectal (CRC). Targeting activating mechanisms, particularly with inhibitors to upstream IκB kinase (IKK) complex, is a promising strategy augment effect chemotherapy. However, clinical success has been limited, largely because low specificity and toxicities tested compounds. In solid cancers, IKKs are driven predominantly by Toll-like receptor (TLR)/IL-1 family members,...
NF-κB transcription factors, driven by the IRAK/IKK cascade, confer treatment resistance in pancreatic ductal adenocarcinoma (PDAC), a cancer characterized near-universal KRAS mutation. Through reverse-phase protein array and RNA sequencing we discovered that IRAK4 also contributes substantially to MAPK activation KRAS-mutant PDAC. ablation completely blocked RAS-induced transformation of human murine cells. Mechanistically, expression mutant stimulated an inflammatory, autocrine IL-1β...
Abstract Persistence of latent HIV-1 in macrophages (MACs) and T-helper lymphocytes (THLs) remain a major therapeutic challenge. Currently available latency reversing agents (LRAs) are not very effective vivo . Therefore, understanding physiologic mechanisms that dictate latency/reactivation reservoirs is clearly needed. Mesenchymal stromal/stem cells (MSCs) regulate the function immune cells; however, their role regulating virus production from latently-infected MACs & THLs known. We...
Prostate cancer (PCa) cells utilize androgen for their growth. Hence, deprivation therapy (ADT) using anti-androgens, e.g. bicalutamide (BIC) and enzalutamide (ENZ), is a mainstay of treatment. However, the outgrowth castration resistant PCa (CRPC) remains significant problem. These CRPC express receptor (AR) intratumoral towards continued growth invasion. Sulforaphane (SFN), naturally occurring isothiocyanate found in cruciferous vegetables, can decrease AR protein levels. In present study,...
Androgen receptor (AR) signaling is fundamental to prostate cancer (PC) progression, and hence, androgen deprivation therapy (ADT) remains a mainstay of treatment. However, augmented AR via both full length (AR-FL) constitutively active splice variants, especially AR-V7, associated with the recurrence castration resistant (CRPC). Oxidative stress also plays crucial role in anti-androgen resistance CRPC outgrowth. We examined whether triterpenoid antioxidant drug, Bardoxolone-methyl, known as...
Abstract Adult-type granulosa cell tumors (aGCT) are rare ovarian sex cord with few effective treatments for recurrent disease. The objective of this study was to characterize the tumor microenvironment (TME) primary and aGCTs identify correlates disease recurrence. Total RNA sequencing (RNA-seq) performed on 24 pathologically confirmed, cryopreserved aGCT samples, including 8 16 tumors. After read alignment quality-control filtering, DESeq2 used differentially expressed genes (DEG) between...
Despite the initial success in treatment of localized prostate cancer (PCa) using surgery, radiation or hormonal therapy, recurrence aggressive tumors dictates morbidity and mortality. Focused ultrasound (FUS) is being tested as a targeted, noninvasive approach to eliminate PCa foci, strategies enhance anticancer potential FUS have high translational value. Since cells utilize oxidative stress (Ox-stress) endoplasmic reticulum (ER-stress) pathways for their survival recurrence, we...
<p>RNA Sequencing QC Metrics</p>
Abstract Introduction: Prostate Cancer (PC) cells utilize androgen for their growth. Therefore, deprivation therapy (ADT) that blocks both systemic production and receptor (AR) signaling remains the mainstay of PC treatment. However, despite efficacy AR antagonists such as bicalutamide (BIC) enzalutamide (ENZ), sub-therapeutic in tumor microenvironments enable selection outgrowth castration resistant (CRPC). Interestingly, CRPC continue to express exploit intratumoral androgen. Hence,...
Abstract Background: A missense mutation in the Forkhead domain-containing FOXL2 (Foxl2 p.C134W) transcription factor is found nearly all adult-type granulosa cell tumors, but oncogenic mechanism of this not known. Other family factors have well-described “pioneer” activity, binding to compacted, nucleosome-bound DNA and increasing accessibility for other regulatory proteins. Objectives: To develop novel culture model systems determine whether Foxl2-C134W has pioneering activity. Methods:...
Abstract Background: Adult-type granulosa cell tumors (AGCT) are rare ovarian sex cord that exhibit near-universal FOXL2 c.C402G (p.Cys134Trp) hotspot mutations. AGCT recurrence is difficult to predict and almost always incurable after relapse. Little known about the relationship between intra-tumor immune stromal composition Objective: To compare global gene expression profiles primary recurrent AGCTs, characterize tumor microenvironment (TME), identify correlates of disease recurrence....
<div>Abstract<p>Adult-type granulosa cell tumors (aGCT) are rare ovarian sex cord with few effective treatments for recurrent disease. The objective of this study was to characterize the tumor microenvironment (TME) primary and aGCTs identify correlates disease recurrence. Total RNA sequencing (RNA-seq) performed on 24 pathologically confirmed, cryopreserved aGCT samples, including 8 16 tumors. After read alignment quality-control filtering, DESeq2 used differentially expressed...
<p>Clinical Characteristics by Patient</p>
<p>Differentially Expressed Genes</p>
<p>S1. Heatmap of unsupervised hierarchical clustering the top 1000 most variable genes.S2. Violin plots showing expression levels genes previously identified as differentially expressed between primary and recurrent tumors in Haltia et al., 2020.S3. Gene set enrichment analysis performed with GO terms KEGG pathways that primarily immune-related hormone-regulated gene sets are altered aGCTs.S4. results enriched significantly comparison tumors.S5. Boxplot fraction each noncancerous cell...