A5042284929- Advanced Proteomics Techniques and Applications
- Environmental Toxicology and Ecotoxicology
- Insect Resistance and Genetics
- Molecular Biology Techniques and Applications
- Aquatic Invertebrate Ecology and Behavior
- Marine Biology and Environmental Chemistry
- Identification and Quantification in Food
- Immunotherapy and Immune Responses
- Metabolomics and Mass Spectrometry Studies
- Genomics and Phylogenetic Studies
- Toxin Mechanisms and Immunotoxins
- Mass Spectrometry Techniques and Applications
- vaccines and immunoinformatics approaches
- T-cell and B-cell Immunology
- Physiological and biochemical adaptations
- Aquaculture Nutrition and Growth
- Marine Bivalve and Aquaculture Studies
- Invertebrate Immune Response Mechanisms
- Viral Infectious Diseases and Gene Expression in Insects
- Supramolecular Self-Assembly in Materials
- Biosensors and Analytical Detection
- Boron Compounds in Chemistry
- Pharmaceutical and Antibiotic Environmental Impacts
- Prostate Cancer Treatment and Research
- Environmental DNA in Biodiversity Studies
CEA Paris-Saclay
2005-2024
CEA Marcoule
2015-2024
Technologies pour la Santé
2018-2024
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2013-2024
Université Paris-Saclay
2020-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2024
Institut de Biologie et Technologies
2013-2022
Laboratoire Innovations Technologiques pour la Détection et le Diagnostic
2017-2021
Service de la Santé Publique
2018-2020
CEA Paris-Saclay - Etablissement de Saclay
2006-2020
Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) has resulted in a pandemic and is continuing to spread rapidly around the globe. No effective vaccine currently available prevent COVID-19, intense efforts are being invested worldwide into development. In this context, all technology platforms must overcome several challenges resulting from use of an incompletely characterized new virus. These include finding right conditions for virus amplification development vaccines...
Abstract In living systems, protein assemblies have essential functions, serving as structural supports, transport highways for molecular cargo, and containers of genetic material. The construction assemblies, which involves control over space time, remains a significant challenge in biotechnology. Here, we show that anionic boron clusters, 3,3′‐commo‐bis[closo‐1,2‐dicarba‐3‐cobaltadodecaborane] (COSAN − ), halogenated closo‐dodecarboranes (B 12 X 2− , X=H, Cl, or I), described...
Abstract NY-ESO-1 is expressed by a broad range of human tumors and often recognized Abs in the sera cancer patients with NY-ESO-1-expressing tumors. The gene also encodes several MHC class I- II-restricted tumor epitopes T lymphocytes. In this study we report one novel pan-MHC peptide sequence, 87–111, that capable binding to multiple HLA-DR HLA-DP4 molecules, including HLA-DRB1*0101, 0401, 0701, 1101 HLA-DPB1*0401 0402 molecules. We demonstrate 87–111 stimulates Th1-type Th-2/Th0-type CD4+...
As a proof of principle, selected reaction monitoring (SRM) mass spectrometry-based methodology was applied to the simultaneous quantification dozens protein biomarkers in caged amphipods (Gammarus fossarum). We evaluated suitability assess complex field contaminations through its application framework regional river network. Thanks high throughput acquisition biomarker levels G. fossarum exposed four reference and 13 contaminated sites, we analyzed individual responses 38 peptides reporting...
Abstract Taking advantage of a large transcriptomic dataset recently obtained in the sentinel crustacean amphipod Gammarus fossarum , we developed an approach based on sequence similarity and phylogenetic reconstruction to identify key players involved endocrine regulation G . Our work identified three genes interest: nuclear receptors RXR E75, regulator broad-complex (BR). Their involvement molting reproduction, along with their sensitivity chemical contamination were experimentally...
Abstract Gammarids are amphipods found worldwide distributed in fresh and marine waters. They play an important role aquatic ecosystems well established sentinel species ecotoxicology. In this study, we sequenced the transcriptomes of a male individual female for seven different taxonomic groups belonging to two genera Gammarus Echinogammarus : fossarum A , G . B C wautieri pulex berilloni marinus These taxa were chosen explore molecular diversity transcribed genes genotyped individuals from...
Taxon-specific proteins from sentinel species have been shown to be among the most relevant monitor biological effects of anthropogenic contaminations. Shotgun proteomics allows high-throughput analysis animal proteomes, but comprehensive coverage remains difficult achieve due wide dynamic range. Here, advantages a new generation mass analyser for environmental toxicoproteomics are documented through identification candidate biomarkers in which genome is as yet unsequenced. A label-free...
Abstract Biological responses of zebra mussel Dreissena polymorpha are investigated to assess the impact contaminants on aquatic organisms and ecosystems. In addition concentrate chemical in their tissues, mussels accumulate several microorganisms such as viruses, protozoa bacteria. order understand molecular mechanisms involved defence against this study aims at identifying immune proteins from D. hemolymph viruses. For purpose, were exposed ex vivo Cryptosporidium parvum RNA poly I:C....
Next generation sequencing and mass spectrometry technologies have recently expanded the availability of whole transcriptomes proteomes beyond classical model organisms in molecular biology, even absence an annotated genome. However, fragmented nature transcriptomic proteomic data reduces ability to interpret data, notably non-model organisms. Network-based approaches may help extracting important biological information from -omics datasets. The reproductive cycle freshwater crustacean...
Proteogenomics, the alliance of proteomics, transcriptomics, genomics and bioinformatics, was first proposed for refining genome annotation using experimental data acquired on gene products. With high-throughput analysis proteins made possible with next-generation tandem mass spectrometers, proteogenomics is greatly improving human per se, helping to decrypt numerous protein modifications occurring during development, aging, illness cancer progression. Further efforts are required obtain a...
The taxol resistance gene TRAG-3 was initially isolated from cancer cell lines that became resistant to in vitro. is a germline Ag expressed by tumors of different histological types including the majority melanoma, breast, and lung cancers. In present study, we report patients with stage IV melanoma breast cancers developed spontaneous IFN-gamma-producing CD4+ T responses against single immunodominant promiscuous peptide epitope presented context multiple HLA-DR molecules. TRAG-3-specific...
Bee venom phospholipase A2 (bvPLA2) is a small, 15kDa enzyme which hydrolyses many phospholipids through interfacial binding. The mutated bvPLA2H34Q (bvPLA2m), in histidine-34 replaced by glutamine, not catalytically active. This protein has been shown to be suitable membrane anchor and suggested as tumor-antigen vector for the development of novel dendritic cell-based vaccines. To confirm this feature, study fusion PNY, composed NY-ESO-1(NY(s)) fused C-terminus bvPLA2m, was engineered....
Proteogenomics is gaining momentum as, today, genomics, transcriptomics, and proteomics can be readily performed on any new species. This approach allows key alterations to molecular pathways identified when comparing conditions. For animals plants, RNA-seq-informed the most popular means of interpreting tandem mass spectrometry spectra acquired for species which genome has not yet been sequenced. It relies high-performance de novo RNA-seq assembly optimized translation strategies. Here,...