A5065897303- Neurobiology and Insect Physiology Research
- Insect Resistance and Genetics
- Insect and Arachnid Ecology and Behavior
- Trace Elements in Health
- Plant and animal studies
- Iron Metabolism and Disorders
- Insect-Plant Interactions and Control
- Animal Behavior and Reproduction
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Insect and Pesticide Research
- Insect Utilization and Effects
- Estrogen and related hormone effects
- Genomics and Chromatin Dynamics
- Environmental DNA in Biodiversity Studies
- Developmental Biology and Gene Regulation
- Heavy Metal Exposure and Toxicity
- Forest Insect Ecology and Management
- Freshwater macroinvertebrate diversity and ecology
- Subterranean biodiversity and taxonomy
- Insect Pheromone Research and Control
- Crustacean biology and ecology
- Coleoptera Taxonomy and Distribution
- Philosophical and Theoretical Analysis
- Biotin and Related Studies
- Effects and risks of endocrine disrupting chemicals
Université Claude Bernard Lyon 1
2009-2024
Centre National de la Recherche Scientifique
2000-2019
École Normale Supérieure de Lyon
2007-2019
Institut de Génomique Fonctionnelle de Lyon
2007-2019
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2009-2017
Canadian Nautical Research Society
2014
Laboratoire de Biologie et Modélisation de la Cellule
2003-2005
Laboratoire de Génétique Cellulaire
2003
Centre de Génétique Moléculaire
2000
University of Leicester
1997
Cladistic analysis allows inference of the structure an ancestral steroid that acts as a ligand for receptor.
Having conquered water surfaces worldwide, the semi-aquatic bugs occupy ponds, streams, lakes, mangroves, and even open oceans. The diversity of this group has inspired a range scientific studies from ecology evolution to developmental genetics hydrodynamics fluid locomotion. However, lack representative strider genome hinders our ability more thoroughly investigate molecular mechanisms underlying processes adaptation diversification within group. Here we report sequencing manual annotation...
Ecdysteroid hormones are major regulators in reproduction and development of insects, including larval molts metamorphosis. The functional ecdysone receptor is a heterodimer ECR (NR1H1) USP-RXR (NR2B4), which the orthologue vertebrate retinoid X receptors (RXR alpha, beta, gamma). Both proteins belong to superfamily nuclear hormone receptors, ligand-dependent transcription factors that share two conserved domains: DNA-binding domain (DBD) ligand-binding (LBD). In order gain further insight...
The Tribolium genome contains 21 nuclear receptors, representing all of the six known subfamilies. This first complete set for a coleopteran species reveals strong conservation number and identity receptors in holometabolous insects. Two novelties are observed: atypical NR0 gene knirps is present only brachyceran flies, while NR2E6 found Apis. Using quantitative analysis evolutionary rate, we discovered that could be divided into two groups. In one group 13 proteins, rates follow trend...
Understanding how the variability of protein structure arises during evolution and leads to new structure–function relationships ultimately promoting evolutionary novelties is a major goal molecular critical for interpreting genome sequences. We addressed this issue using ecdysone receptor (ECR), developmental factor that controls development reproduction arthropods. The functional ECR heterodimer two nuclear receptors: ECR, which binds ecdysteroids, its obligatory partner ultraspirade...
Naturalists have been fascinated for centuries by animal colors and color patterns. While widely studied at the adult stage, we know little about patterns in embryo. Here, study a trait consisting of coloration that is specific to embryo absent from postembryonic stages water striders (Gerromorpha). By combining developmental genetics with chemical phylogenetic analyses across broad sample species, uncovered mechanisms underlying emergence diversification embryonic this group insects. We...
The effects of endocrine disrupting chemicals (EDCs) on reproduction are well known, whereas their developmental much less characterized. However, exposure to disruptors during organogenesis may lead deleterious and permanent problems later in life. Zebrafish (Danio rerio) transgenic lines expressing the green fluorescent protein (GFP) specific organs tissues powerful tools uncover defects elicited by EDCs. Here, we used seven visualize vivo whether a series EDCs other pharmaceutical...
Abstract Taking advantage of a large transcriptomic dataset recently obtained in the sentinel crustacean amphipod Gammarus fossarum , we developed an approach based on sequence similarity and phylogenetic reconstruction to identify key players involved endocrine regulation G . Our work identified three genes interest: nuclear receptors RXR E75, regulator broad-complex (BR). Their involvement molting reproduction, along with their sensitivity chemical contamination were experimentally...
Extensive sequence analysis of the developmental gene hunchback and its 5' 3' regulatory regions in Drosophila melanogaster, virilis, Musca domestica, Tribolium castaneum, using a variety computer algorithms, reveals high simplicity probably generated by slippage-like mechanisms turnover. No are entirely refractory to action slippage, although density composition simple motifs varies from region region. Interestingly, flanking share short repetitive despite their separation itself, different...
Abstract In arthropods, the regulation by ecdysteroids is mediated heterodimer between ecdysone receptor (ECR; NR1H1) and ultraspiracle (USP/RXR; NR2B4) nuclear receptors. Both ECR USP/RXR ligand‐binding domains experienced a strong acceleration of evolutionary rate in Diptera Lepidoptera, which belong to superorder Mecopterida. We performed phylogenetic analysis 28 30 protein sequences from 36 arthropod species, including representatives Trichoptera, Mecoptera Siphonaptera. Our data show...
The metallothionein system in Drosophila melanogaster is composed of two genes, Mtn and Mto. In order to compare the induction properties these we transformed D. with P-element vectors containing Adh lacZ reporter genes under control Mto promoters, respectively. transgenes are mainly expressed digestive tract. However, expression has been detected also fat body. respond differently metallic, heat-shock oxidative stresses. These data confirm that both part functionally different.
Abstract Background The maintenance of biological systems requires plasticity and robustness. function the ecdysone receptor, a heterodimer composed nuclear receptors ECR (NR1H1) USP (NR2B4), was maintained in insects despite dramatic divergence that occurred during emergence Mecopterida. This receptor is therefore good model to study evolution plasticity. We tested hypothesis selection has shaped Ligand-Binding Domain (LBD) Results isolated usp cox1 several species Drosophilidae,...
Abstract Developmental expression of the Drosophila melanogaster metallothionein Mtn gene has been analysed. Transcripts this accumulate during vitellogenic phase oogenesis in a ring follicular cells at oocyte‐nurse cell margin and surrounding oocyte. There is also strong second half embryogenesis hemocytes, endoderm midgut, Malpighian tubules. A banded pattern observed transiently midgut stage 13. The two alleles, 1 .3 , show quantitative differences their patterns. Copper intoxication...
Abstract The semi-aquatic bugs conquered water surfaces worldwide and occupy ponds, streams, lakes, mangroves, even open oceans. As such, they inspired a range of scientific studies from ecology evolution to developmental genetics hydrodynamics fluid locomotion. However, the lack representative strider genome hinders thorough investigations mechanisms underlying processes adaptation diversification in this group. Here we report sequencing manual annotation Gerris buenoi (G. buenoi) genome,...