A5001943591- Developmental Biology and Gene Regulation
- Hemiptera Insect Studies
- Phytoplasmas and Hemiptera pathogens
- Research on scale insects
- Neurobiology and Insect Physiology Research
- Insect and Arachnid Ecology and Behavior
- Echinoderm biology and ecology
- Insect Resistance and Genetics
- RNA Research and Splicing
- Animal Behavior and Reproduction
- Ocean Acidification Effects and Responses
- Marine Biology and Environmental Chemistry
- Invertebrate Immune Response Mechanisms
- Plant Molecular Biology Research
- Insect symbiosis and bacterial influences
- Marine Ecology and Invasive Species
- Genomics and Chromatin Dynamics
- Cephalopods and Marine Biology
- Planarian Biology and Electrostimulation
- Marine and coastal plant biology
- BRCA gene mutations in cancer
- Freshwater macroinvertebrate diversity and ecology
- CRISPR and Genetic Engineering
- Congenital heart defects research
- RNA and protein synthesis mechanisms
University of Maryland, College Park
2016-2023
Carnegie Mellon University
2011-2017
University of Pittsburgh
2009
Many patients remain without a diagnosis despite extensive medical evaluation. The Undiagnosed Diseases Network (UDN) was established to apply multidisciplinary model in the evaluation of most challenging cases and identify biologic characteristics newly discovered diseases. UDN, which is funded by National Institutes Health, formed 2014 as network seven clinical sites, two sequencing cores, coordinating center. Later, central biorepository, metabolomics core, organisms screening center were added.
Abstract Background Halyomorpha halys (Stål), the brown marmorated stink bug, is a highly invasive insect species due in part to its exceptionally high levels of polyphagy. This also nuisance overwintering human-made structures. It has caused significant agricultural losses recent years along Atlantic seaboard North America and continental Europe. Genomic resources will assist with determining molecular basis for this species’ feeding habitat traits, defining potential targets pest...
A great challenge in development biology is to understand how interacting networks of regulatory genes can direct the often highly complex patterning cells a 3D embryo. Here, we detail gene network that describes distribution ciliary band-associated neurons bipinnaria larva sea star. This larva, typically for ancestral deuterostome dipleurula larval type it represents, forms two loops bands extend across much anterior-posterior and dorsal-ventral ectoderm. We show star first likely uses...
Having conquered water surfaces worldwide, the semi-aquatic bugs occupy ponds, streams, lakes, mangroves, and even open oceans. The diversity of this group has inspired a range scientific studies from ecology evolution to developmental genetics hydrodynamics fluid locomotion. However, lack representative strider genome hinders our ability more thoroughly investigate molecular mechanisms underlying processes adaptation diversification within group. Here we report sequencing manual annotation...
Gene regulatory networks (GRNs) describe the progression of transcriptional states that take a single-celled zygote to multicellular organism. It is well documented GRNs can evolve extensively through mutations cis-regulatory modules (CRMs). Transcription factor proteins bind these CRMs may also produce novelty. Coding changes are considered be rarer, however, because transcription factors multifunctional and hence more constrained in ways will not widespread detrimental effects. Recent...
Sea stars and sea urchins are model systems for interrogating the types of deep evolutionary changes that have restructured developmental gene regulatory networks (GRNs). Although cis -regulatory DNA evolution is likely predominant mechanism change, it was recently shown Tbrain, a Tbox transcription factor protein, has evolved changed preference low-affinity, secondary binding motif. The primary, high-affinity motif conserved. To date, however, no genome-wide comparisons been performed to...
Echinoderms have long been a favorite model system for studies of reproduction and development, more recently the study gene regulation evolution developmental processes. The sea star, Patiria miniata, is gaining prevalence as these types which were previously performed almost exclusively in urchins, Strongylocentrotus purpuratus Lytechinus variegatus. An advantage systems ease producing modified embryos particular up or downregulated, labeling group cells, introducing reporter gene. A...
The discovery of pair-rule genes (PRGs) in Drosophila revealed the existence an underlying two-segment-wide prepattern directing embryogenesis. milkweed bug Oncopeltus fasciatus, a hemimetabolous insect, is more representative arthropod: most its segments form sequentially after gastrulation. Here, we report expression and function orthologs complete set nine PRGs Seven Of-PRG-orthologs are expressed stripes primordia every segment, rather than other segment; Of-runt PR-like several also...
The tumor suppressor Adenomatous polyposis coli (APC) negatively regulates Wnt signaling through its activity in the destruction complex. APC binds directly to main effector of pathway, β-catenin (βcat, Drosophila Armadillo), and helps target it for degradation. In vitro studies demonstrated that a nonphosphorylated 20-amino-acid repeat (20R) βcat N-terminal extended region 20R. When phosphorylated, phospho-region an 20R also affinity is significantly increased. These distinct APC-βcat...
Abstract Regulatory genes are often multifunctional and constrained, which results in evolutionary conservation. It is difficult to understand how a regulatory gene could be lost from one species’ genome when it essential for viability closely related species. The paired classic Drosophila pair-rule gene, required formation of alternate body segments diverse insect Surprisingly, was mosquitoes without disrupting patterning. Here, we demonstrate that family member, gooseberry , has acquired...
Abstract The semi-aquatic bugs conquered water surfaces worldwide and occupy ponds, streams, lakes, mangroves, even open oceans. As such, they inspired a range of scientific studies from ecology evolution to developmental genetics hydrodynamics fluid locomotion. However, the lack representative strider genome hinders thorough investigations mechanisms underlying processes adaptation diversification in this group. Here we report sequencing manual annotation Gerris buenoi (G. buenoi) genome,...
Abstract The discovery of pair-rule genes (PRGs) in Drosophila revealed the existence an underlying two-segment-wide prepattern directing embryogenesis. milkweed bug Oncopeltus , a hemimetabolous insect, is more representative arthropod: most its segments form sequentially after gastrulation. Here we report expression and function orthologs complete set nine PRGs . Seven Of -PRG-orthologs are expressed stripes primordia every segment, rather than every-other Of-runt PR-like, several also...
Abstract How neural stem cells generate the correct number and type of differentiated neurons in appropriate places is an important question developmental biology. Although nervous systems are diverse across phyla, many taxa have a larva that forms anterior concentration neurons, or apical organ. The these organs highly variable. We show neurogenesis sea star larvae begins with soxc -expressing multipotent progenitors. These give rise to restricted progenitors express lhx2/9 . Soxc - capable...
Echinoderms have long been a favorite model system for studies of reproduction and development, more recently the study gene regulation evolution developmental processes. The sea star, Patiria miniata, is gaining prevalence as these types which were previously performed almost exclusively in urchins, Strongylocentrotus purpuratus Lytechinus variegatus. An advantage systems ease producing modified embryos particular up or downregulated, labeling group cells, introducing reporter gene. A...
Abstract The gene regulatory network for segmentation in arthropods offers valuable insights into how networks evolve owing to the breadth of species examined and extremely detailed knowledge gained model organism Drosophila melanogaster . These studies have shown that 's represents a derived state acquired changes accelerate segment patterning, whereas most insects specify segments gradually as embryo elongates. Such heterochronic shifts potentially emerged multiple times within...
Abstract Culex pipiens form and Cx. molestus differ in their ability to produce eggs without a bloodmeal. Autogenous mosquitoes, such as the bioform of , depend on nutrition acquired larvae instead bloodmeal fuel energy intensive process vitellogenesis, which requires abundant production yolk proteins. In anautogenous mosquito systems, ovary ecdysteroidogenic hormone (OEH) insulin-like peptides (ILPs) transduce nutritional signals trigger egg maturation response It is unclear what extent...