A5003941210- Neurobiology and Insect Physiology Research
- Muscle activation and electromyography studies
- Genetic Neurodegenerative Diseases
- Children's Physical and Motor Development
- Vestibular and auditory disorders
- Erythrocyte Function and Pathophysiology
- Nerve injury and regeneration
- Muscle Physiology and Disorders
- Ion channel regulation and function
- Pain Mechanisms and Treatments
- Ion Channels and Receptors
- Cancer-related Molecular Pathways
- Microtubule and mitosis dynamics
- Axon Guidance and Neuronal Signaling
- Neurogenesis and neuroplasticity mechanisms
- Genomics and Chromatin Dynamics
- Ubiquitin and proteasome pathways
- Neuroscience and Neural Engineering
- Respiratory and Cough-Related Research
- Zebrafish Biomedical Research Applications
- 3D Printing in Biomedical Research
- Hearing, Cochlea, Tinnitus, Genetics
- Action Observation and Synchronization
- Genetics and Physical Performance
- Cellular Mechanics and Interactions
Columbia University Irving Medical Center
2019-2025
Columbia University
2006-2024
Howard Hughes Medical Institute
2006-2015
Institute of Molecular Biology and Biophysics
2015
Massachusetts General Hospital
1995-2000
The organization of spinal reflex circuits relies on the specification distinct classes proprioceptive sensory neurons (pSN), but factors that drive such diversity remain unclear. We report here pSNs supplying skeletal muscles differ in their dependence ETS transcription factor Etv1 for survival and differentiation. status Etv1-dependence is linked to location proprioceptor muscle targets: innervating hypaxial axial depend critically survival, whereas those certain limb are resistant...
Proprioceptive feedback mainly derives from groups Ia and II muscle spindle (MS) afferents group Ib Golgi tendon organ (GTO) afferents, but the molecular correlates of these three afferent subtypes remain unknown. We performed single cell RNA sequencing genetically identified adult proprioceptors uncovered five molecularly distinct neuronal clusters. Validation cluster-specific transcripts in dorsal root ganglia skeletal demonstrates that two clusters correspond to MS GTO proprioceptors,...
Cell proliferation and cell fate specification are under strict spatiotemporal control in the developing Drosophila eye. Cells excluded from five-cell preclusters synchronously enter a single additional cycle, second mitotic wave, after which remaining cells sequentially recruited. When wave was blocked with human cyclin-dependent kinase inhibitor p21 CIP1/WAF1 , each type still specified. Hence, determination is regulated independently of division pattern precursor cells. However, needed to...
Peripheral sensory neuropathy (PSN) is associated with several devastating neurological conditions, yet effective strategies to prevent or alleviate the consequences of PSN are nearly non-existent. A major challenge in development better therapeutic interventions lack appropriate human model systems. Human induced pluripotent stem cell (hiPSC)-derived somatosensory neurons present a promising strategy overcome this issue but remain limited translational utility, part due low efficiency and...
Mechanoreception is an essential feature of many sensory modalities. Nevertheless, the mechanisms that govern conversion a mechanical force to distinct patterns action potentials remain poorly understood. Proprioceptive mechanoreceptors reside in skeletal muscle and inform nervous system position body limbs space. We show here Whirlin/Deafness autosomal recessive 31 (DFNB31), PDZ-scaffold protein involved vestibular auditory hair cell transduction, also expressed by proprioceptive neurons...
Proprioceptive feedback from Group Ia/II muscle spindle afferents and Ib Golgi tendon is critical for the normal execution of most motor tasks, yet how these distinct proprioceptor subtypes emerge during development remains poorly understood. Using molecular genetic approaches in mice either sex, we identified 24 transcripts that have not previously been associated with a identity. Combinatorial expression analyses markers reveal at least three molecularly subtypes. In addition, find 12 are...
Abstract Anatomical and physiological analyses have long revealed differences between proprioceptive groups Ia, II, Ib sensory neurons, yet the molecular correlates of these three muscle afferent subtypes remain unknown. We performed single cell RNA sequencing genetically identified adult proprioceptors and, using unbiased bioinformatics approaches, detected five molecularly distinct neuronal clusters. Validation cluster-specific transcripts in dorsal root ganglia (DRG) skeletal provides...
Abstract Friedreich’s ataxia (FRDA) is one of the most common hereditary ataxias. It caused by a GAA repeat in first intron FXN gene, which encodes an essential mitochondrial protein. Patients suffer from progressive motor dysfunction due to degeneration mechanoreceptive and proprioceptive neurons dorsal root ganglia (DRG) cerebellar dentate nucleus neurons, especially at early disease stages. Postmortem analyses FRDA patients also indicate pathological changes cortex including projection...