A5031736889- Soft tissue tumor case studies
- Protein Structure and Dynamics
- Enzyme Structure and Function
- Computational Drug Discovery Methods
- Sarcoma Diagnosis and Treatment
- Soft tissue tumors and treatment
- Hormonal Regulation and Hypertension
- RNA and protein synthesis mechanisms
- Photosynthetic Processes and Mechanisms
- Bacterial biofilms and quorum sensing
- Blood Pressure and Hypertension Studies
- Microbial Metabolic Engineering and Bioproduction
- Molecular spectroscopy and chirality
- Bacterial Genetics and Biotechnology
- Metabolomics and Mass Spectrometry Studies
- ATP Synthase and ATPases Research
- Metabolism and Genetic Disorders
- Machine Learning in Bioinformatics
- Enterobacteriaceae and Cronobacter Research
- Neonatal and fetal brain pathology
- Traditional Chinese Medicine Studies
- Vibrio bacteria research studies
- Ion channel regulation and function
- Listeria monocytogenes in Food Safety
- Lung Cancer Research Studies
Duke Medical Center
2019-2024
Desmoid Tumor Research Foundation
2019-2023
Granta Design (United Kingdom)
2020
RTI International
2016-2018
Botsford Hospital
2014
National Institute of Environmental Health Sciences
2009-2010
University of Nebraska–Lincoln
2005-2010
Kelly Services (United States)
2009-2010
Rutgers, The State University of New Jersey
2006
Abstract Rapid and accurate functional assignment of novel proteins is increasing in importance, given the completion numerous genome sequencing projects vastly expanding list unannotated proteins. Traditionally, global primary‐sequence structure comparisons have been used to determine putative function. These approaches, however, do not emphasize similarities active site configurations that are fundamental a protein's activity highly conserved relative more variable structural features. The...
Barth Syndrome is the only known Mendelian disorder of cardiolipin remodeling, with characteristic clinical features cardiomyopathy, skeletal myopathy, and neutropenia. While primary biochemical defects reduced mature increased monolysocardiolipin are well-described, much downstream dysregulation has not been uncovered, biomarkers limited. In order to further expand upon knowledge abnormalities in Syndrome, we analyzed metabolite profiles plasma from a cohort individuals compared age-matched...
An abundance of protein structures emerging from structural genomics and the Protein Structure Initiative (PSI) are not amenable to ready functional assignment because a lack sequence homology proteins known function. We describe high-throughput NMR methodology (FAST-NMR) annotate biological function novel through analysis protein−ligand interactions. This is based on basic tenets biochemistry where with similar functions will have active sites exhibit ligand binding interactions, despite...
Desmoid tumors (aggressive fibromatosis) are locally invasive soft tissue that lack the ability to metastasize. There no directed therapies or standard treatment plan, and chemotherapeutics, radiation, surgery often have temporary effects. The majority of desmoid related T41A S45F mutations beta-catenin encoding gene (CTNNB1). Using broad spectrum metabolomics, differences were investigated between paired normal fibroblast tumor cells from affected patients. identified, also, in metabolomics...
We identified a homologue of the molluscan acetylcholine-binding protein (AChBP) in marine polychaete Capitella teleta, from annelid phylum. The amino acid sequence C. teleta AChBP (ct-AChBP) is 21−30% identical with those known AChBPs. Sequence alignments indicate that ct-AChBP has shortened Cys loop compared to other receptors, and variation on conserved triad, which associated ligand binding AChBPs nicotinic ACh receptor (nAChR) α subunits. Within D ct-AChBP, aromatic residue (Tyr or Trp)...
Circulating tumor DNA (ctDNA)-based molecular profiling is rapidly gaining traction in clinical practice of advanced cancer patients with multi-gene next-generation sequencing (NGS) panels. However, outcomes remain poorly described and deserve further validation personalized treatment genomic alterations detected plasma ctDNA. Here, we describe the outcomes, disease control rate (DCR) at 3 months progression-free survival (PFS) oncogenic-addicted NSCLC actionable identified by ctDNA liquid...
A multi-step NMR based screening assay is described for identifying and evaluating chemical leads their ability to bind a target protein. The provides structure-related information while being an integral part of structure drug discovery design program. fundamental principle the combine distinct 1D 2D techniques, in such manner, that inherent strengths weakness associated with each technique complementary other screen. By taking advantage combined experiments, it possible minimize protein...
In the past few years, NMR has been extensively utilized as a screening tool for drug discovery using various types of compound libraries. The designs specific chemical libraries that utilize fragment-based approach based on drug-like characteristics have previously reported. this article, new type library will be described focuses aiding in functional annotation novel proteins identified from ongoing genomics efforts. is comprised small molecules with known biological activity such as:...
Pseudomonas aeruginosa is the prototypical biofilm-forming gram-negative opportunistic human pathogen. P. causatively associated with nosocomial infections and cystic fibrosis. Antibiotic resistance in some strains adds to inherent difficulties that result from biofilm formation when treating infections. Transcriptional profiling studies suggest widespread changes proteome during quorum sensing development. Many of proteins found be upregulated these processes are poorly characterized a...
The solution structure of the Bacillus subtilis protein YndB has been solved using NMR to investigate proposed biological functions. exhibits helix-grip fold, which consists a β-sheet with two small and one long α-helix, forming hydrophobic cavity that preferentially binds lipid-like molecules. Sequence comparisons proteins from eukaryotes, prokaryotes, archaea suggest is very similar eukaryote Aha1, middle domain Hsp90 induces ATPase activity. On basis these similarities, classified as...
e23549 Background: Desmoid tumors are a benign sarcoma diagnosed in 4-5 patients per million each year. The Tumor Research Foundation (DTRF) launched the patient registry and natural history study (NHS) 2017. This is retrospective analysis of diagnostic data collected, tumor location, rates misdiagnosis, how genetics testing being incorporated into clinical practice, additional trial participation. Methods: NHS September 2017 contains 15 surveys covering diagnostics, disease, treatment, care...
Desmoid tumors are locally invasive sarcoma, affecting 5-6 individuals out of 1,000,000 per year. The desmoid have high rates recurrence after resection and can lead to significant deterioration the quality life patients. There is a need for better understanding tumors' patient experience from first symptoms through diagnosis, disease monitoring, clinical treatment options. With National Organization Rare Disorders, Tumor Research Foundation Natural History Study was designed be collected...
Desmoid tumors (aggressive fibromatosis) can negatively affect patients' lives and overall quality of life due to the associated symptoms complications. This analysis describes patient-reported symptom severity impact desmoid on participants' lives. is an global, survey-based Tumor Research Foundation (DTRF) Natural History Study using data collected from Sept 2017 Aug 2023. Participants and/or their caregivers reported a tumor diagnosis, symptoms, Symptom were evaluated tumor-specific...
Patients with desmoid tumors (aggressive fibromatosis) have several treatment options, and individual experiences are vast varied. This analysis describes the landscape for patients after diagnosis. is a descriptive of global, survey-based Desmoid Tumor Research Foundation (DTRF) Natural History Study using data collected from Sept 2017 to Aug 2023. Participants and/or their caregivers reported tumor diagnosis treatment. The N values vary these analyses due incomplete data. Of 383...
11513 Background: Desmoid fibromatosis (DF) often occurs during pregnancy/peripartum. Guidance for planning new pregnancies active surveillance or following DF resection has been limited. Thus, we sought to evaluate outcomes and decision making in the peripartum. Methods: Women of child-bearing age with diagnosed between 2000 2020 were interviewed about procreation decisions, a multicenter retrospective observational study (NCT05284305). Pregnancies simultaneous after diagnosis analyzed....