A5003186673- Protein Interaction Studies and Fluorescence Analysis
- Cannabis and Cannabinoid Research
- Protein Structure and Dynamics
- Alzheimer's disease research and treatments
- Perovskite Materials and Applications
- Conducting polymers and applications
- Quantum Dots Synthesis And Properties
- Neurotransmitter Receptor Influence on Behavior
- Chalcogenide Semiconductor Thin Films
- Biochemical effects in animals
- Proteins in Food Systems
- Trace Elements in Health
- RNA Interference and Gene Delivery
- Collagen: Extraction and Characterization
- Surfactants and Colloidal Systems
- Spectroscopy Techniques in Biomedical and Chemical Research
- Advanced Glycation End Products research
- Sensorless Control of Electric Motors
- Pharmacological Receptor Mechanisms and Effects
- Pain Mechanisms and Treatments
- Multilevel Inverters and Converters
- Electric Motor Design and Analysis
- Computational Drug Discovery Methods
- Enzyme Structure and Function
- Fern and Epiphyte Biology
Indian Institute of Technology Bombay
2019-2024
Maulana Azad National Institute of Technology
2019-2024
Indian Institute of Technology Kanpur
2021-2023
Technion – Israel Institute of Technology
2019
Indian Institute of Technology Kharagpur
1965-2018
Virginia Commonwealth University
2012-2016
The University of Texas Health Science Center at San Antonio
2015
West Virginia University
2015
Scripps Research Institute
2015
Medical College of Wisconsin
2015
Since monoacylglycerol lipase (MAGL) has been firmly established as the predominant catabolic enzyme of endocannabinoid 2-arachidonoylglycerol (2-AG), a great need emerged for development highly selective MAGL inhibitors. Here, we tested in vivo effects one such compound, KML29 (1,1,1,3,3,3-hexafluoropropan-2-yl 4-(bis(benzo[d][1,3]dioxol-5-yl)(hydroxy)methyl)piperidine-1-carboxylate).
Despite having long excited carrier lifetimes and high mobilities in hybrid halide perovskite materials, conventional (n-i-p) devices exhibit significant interfacial nonradiative recombination losses that are little understood but limit the radiative efficiency overall open-circuit potential. In this Letter, we reveal process of spiro-OMeTAD coating on gives rise to buried defect states, which detrimental devices' operational stability. We subsequently report a method passivate these...
Glycation causes severe damage to protein structure that could lead amyloid formation in special cases. Here this report, we have shown for the first time hen egg white lysozyme (HEWL) does not undergo even after prolonged glycation presence of D-glucose, D-fructose and D-ribose. Cross-linked oligomers were formed all cases ribose was found be most potent among three sugars. Ribose mediated oligomers, however, exhibit Thioflavin T binding properties although microscopic images clearly show...
The aggregation process in proteins is governed by several factors such as temperature, pH, presence of electrolytes, denaturants, and metal ions. Here, we report the role Cu(II) inducing rapid fibrillation human serum albumin. We have monitored this via UV−vis spectroscopy, fluorescence circular dichroism, ζ-potential measurements, electron paramagnetic resonance studies, microscopy, field emission scanning microscopy. Images show a fibrillar network albumin 60% ethanol incubated at 65 °C...
Inhibition of fatty acid amide hydrolase (FAAH) or monoacylglycerol lipase (MAGL), the primary hydrolytic enzymes for respective endocannabinoids <i>N</i>-arachidonoylethanolamine (AEA) and 2-arachidonylglycerol (2-AG), produces antinociception but with minimal cannabimimetic side effects. Although selective inhibitors either enzyme often show partial efficacy in various nociceptive models, their combined blockade elicits augmented antinociceptive effects, effects emerge. Moreover, complete...
Inhibition of diacylglycerol lipase (DGL)β prevents LPS-induced pro-inflammatory responses in mouse peritoneal macrophages. Thus, the present study tested whether DGLβ inhibition reverses allodynic mice LPS model inflammatory pain, as well neuropathic pain models.Initial experiments examined cellular expression and mediators within LPS-injected paw pad. DAGL-β (-/-) or wild-type treated with inhibitor KT109 were assessed pain. Additional studies locus action for KT109-induced...
Hen egg white lysozyme (HEWL) adopts a molten globule-like state at high pH (~12.75) and is found to form amyloid fibrils alkaline pH. Here, we report that Cu(II) inhibits self-association of HEWL 12.75 both 37 65 °C. A significant reduction in Thioflavin T fluorescence intensity, attenuation β-sheet content hydrophobic exposure were observed with increasing stoichiometry. Electron paramagnetic resonance spectroscopy suggests 4N type coordination pattern around during fibrillation. also...
Protein self-association leads to toxic filamentous plaques believed be the key reason behind several neurodegenerative diseases. Stabilization of fibrillar architecture is guided by forces; primarily electrostatic, hydrophobic, and extent hydrogen bonding. In this study, we experimentally demonstrate, for first time, effect varying static electric fields on preformed fibrils human serum albumin (HSA) in an electrowetting dielectric type setup using spectroscopic microscopic techniques. We...
Protein aggregation is related to a series of pathological disorders the main cause which are fibrillar species generated during process. Human serum albumin (HSA) undergoes rapid fibrillation in presence Cu(II) at pH 7.4 60% ethanol after 6-h incubation (∼65 °C) followed by room temperature incubation. Here, we have investigated effect stoichiometric variation on self-assembly HSA using Congo red and thioflavin T dye-binding studies, circular dichroism spectroscopy, Fourier transform...
Encapsulates are, in general, the passive components of any photovoltaic device that provides required shielding from externally stimulated degradation. Here we provide comprehensive physical insight depicting a rather non-trivial active nature, contrast to supposedly passive, atomic layer deposition (ALD) grown Al2O3 encapsulate on hybrid perovskite [(FA0.83MA0.17)0.95Cs0.05PbI2.5Br0.5] having configuration: glass/FTO/SnO2/perovskite/spiro-OMeTAD/Au/(±) Al2O3. By combining various...
During the past several years, studies on protein aggregation process in presence of cosolvents/ co-solutes have been looked into which provides significant insight stability proteins a crowded cellular milieu. Here, present report we investigated fibrillation human serum albumin (HSA) under mixed aqueous-ethanol solvent conditions at two different temperatures (37 °C and 65 °C). Self-association was monitored using various spectroscopic microscopic techniques. Results obtained from detailed...