A5027351937- Bacterial Identification and Susceptibility Testing
- Antibiotic Resistance in Bacteria
- Antimicrobial Resistance in Staphylococcus
- Pneumonia and Respiratory Infections
- Antibiotics Pharmacokinetics and Efficacy
- Streptococcal Infections and Treatments
- Pneumocystis jirovecii pneumonia detection and treatment
- Respiratory viral infections research
- Enterobacteriaceae and Cronobacter Research
- Bacterial biofilms and quorum sensing
- Cervical Cancer and HPV Research
- Bacterial Infections and Vaccines
- Infective Endocarditis Diagnosis and Management
- Clostridium difficile and Clostridium perfringens research
- Hepatitis B Virus Studies
- Viral gastroenteritis research and epidemiology
- Microscopic Colitis
- Appendicitis Diagnosis and Management
- Mycobacterium research and diagnosis
- Urinary Tract Infections Management
- Pharmaceutical and Antibiotic Environmental Impacts
- Antibiotic Use and Resistance
- Actinomycetales infections and treatment
- Infections and bacterial resistance
- Medical and Biological Ozone Research
Universidade Federal do Rio Grande do Sul
2014-2024
Instituto de Ciências Farmacêuticas
2024
Universidade Federal do Rio Grande
2021
Hospital Moinhos de Vento
2018-2019
Universidade Federal de Ciências da Saúde de Porto Alegre
2006-2017
University of Health Science
2014
Pontifícia Universidade Católica do Rio Grande do Sul
2009
Fundação Faculdade de Medicina
2006
Hospital Mãe de Deus
2004
Despite the increasing importance of Enterococcus as opportunistic pathogens, their virulence factors are still poorly understood. This study determines frequency in clinical and commensal isolates from inpatients Porto Alegre, Brazil. Fifty were analysed presence gelE, asa1 esp genes was determined. Gelatinase activity biofilm formation also tested. The clonal relationships among evaluated using pulsed-field gel electrophoresis. asa1, gelE identified 38%, 60% 76% all isolates, respectively....
Abstract For Brazil, there are no nationwide data on HPV prevalence against which the impact of immunization program can be measured in future. Therefore, we aim to evaluate genital infection among adolescents and young adults Brazil. A cross-sectional, multicentric, survey was conducted between September 2016 November 2017. Sexually active unvaccinated women men aged 16 25 years old were recruited from 119 public primary care units, including all 26 state capitals Federal District. All...
To reduce the burden of pneumococcal diseases, different formulations conjugate vaccines (PCV) have been introduced in many countries. In Brazil, PCV10 has available since 2010. We aimed to analyze serotype and genetic composition invasive pneumococci from Brazil pre- post- vaccination periods (2007–2012). Antibiotic susceptibility was determined genotypes macrolide fluoroquinolone resistance were characterized. The isolates most frequent serotypes by multilocus sequence typing. study...
Introduction Human papillomavirus (HPV) is associated with the development of genital warts and different types cancer, including virtually all cervical cancers a considerable number penile, anal oropharyngeal cancers. Data regarding prevalence HPV infection in Brazil are limited fragmented. We aim to determine sexually active women men aged 16–25 years investigate regional differences virus types. Methods analysis This nationwide, multicentric, cross-sectional, prospective study that will...
Abstract Use of pneumococcal conjugate vaccines has caused emergence non-vaccine serotypes. No Brazilian data specifically about serotype 19A are available. We aimed to evaluate the frequency occurrence, susceptibility profile and molecular epidemiology before after vaccine introduction in Brazil. Pneumococcal identification was performed by conventional method. Strain determined multiplex polymerase chain reaction (PCR) and/or Quellung reaction. Resistance Etest ® PCR determine presence...
We described a case of 27-year old male patient with skin and soft tissue infection neoplastic lesion caused by Corynebacterium striatum, an organism which has been rarely as human pathogen. Identification was confirmed DNA sequencing. Successful treatment penicillin achieved. The role the C. striatum emerging opportunistic pathogen is discussed.
To evaluate the susceptibility patterns among Streptococcus pneumoniae recovered during years 2010-2012 and to correlate these with serotypes.Pneumococci from invasive sites were serotyped by sequential multiplex PCR and/or Quellung reaction. Etest strips used determine minimal inhibitory concentrations, Clinical Laboratory Standards Institute (CLSI) guidelines for interpretation. Genetic determinants of macrolide resistance assessed PCR, occurrence D phenotype was analyzed following...
In Porto Alegre (South Brazil), since the first VRE isolation in 2000 until middle of last decade, epidemiology enterococcal infections presented peculiarity that, as opposed to other regions country, almost all were E. faecalis. The aim this study was investigate microbiological and epidemiological characteristics a outbreak that occurred between August 2010 September 2011 Alegre, South Brazil.Twenty-nine isolates from inpatients Mãe de Deus Hospital identified characterized for their...
The sexual behaviors of 15- to 24-year-olds increase the risk this population acquire sexually transmitted infections (STIs). present study aimed describe behavior in transition adulthood Brazilian and its association with STI history.We analyzed cross-sectional data collected from 8562 active women men who participated National Survey Human Papillomavirus Prevalence (POP-Brazil). This large-scale survey enrolled participants 26 capitals Federal District. Professionals primary care...
INTRODUCTION: Methicillin-Resistant Staphylococcus aureus (MRSA) presenting reduced susceptibility to vancomycin has been associated therapeutic failure. Some methods used by clinical laboratories may not be sufficiently accurate detect this phenotype, compromising results and the outcome of patient. OBJECTIVES: To evaluate performance in detection MIC values among isolates MRSA. MATERIAL AND METHODS: The Vancomycin Minimal Inhibitory Concentration was determined for 75 MRSA from inpatients...
Background Vaccination using the 10-valent conjugate vaccine (PCV-10) was introduced into Extended Program on Immunization in Mozambique March 2013, however its impact pediatric pneumococcal meningitis is unknown. In this study, we assessed for first time of PCV10 burden children less than 5 years age at three largest hospitals Mozambique. Method Between 2013 and December 2015, a total 744 cerebrospinal fluid (CSF) samples were collected from eligible children, which 160 (21.5%) positive S....
Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most frequently isolated agents in both nosocomial and community settings. It a constant challenge for antibacterial therapy. Therefore, it becomes essential to understand epidemiology MRSA isolates institution and/or region guide empirical The objective this study was evaluate epidemiological characteristics state Santa Catarina, Brazil, determine if there clonal spread. We evaluated 124 clinical obtained from various...
Coagulase-negative staphylococci (CNS) are the major cause of nosocomial infections. Methicillin-resistant strains particularly important because they narrow therapeutic options. Detecting methicillin resistance among CNS has been a challenge for years. The objective this study was to determine accuracy an agar screening test (0.6 and 4 microg oxacillin ml(-1)), disc diffusion automated MicroScan system characterize CNS. One hundred seventy five were analysed: 41.1 % Staphylococcus...
According to the National Committee for Clinical Laboratory Standards (NCCLS, 2004), a method evaluate inducible clindamycin resistance in accordance with an approach of disks erythromycin and - D test has been reported. We analyzed performance this 200 coagulase negative staphylococci (CoNS) strains obtained from blood cultures hospitalized patients at general hospital Southern Brazil. Twenty-seven clinical isolates suitable profile (erythromycin-resistant clindamycin-susceptible) were...
Detecting carbapenemase-associated carbapenem resistance is a subject of major clinical and epidemiological concern as it influences therapeutic choice. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been proposed means to assess bacterial mechanisms. We aimed detect the KPC enzyme directly from positive blood cultures using MALDI-TOF MS. To do so, 102 Enterobacteria were evaluated, including 59 blaKPC positives. Proteins extracted formic...
Purpose. Invasive pneumococcal disease (IPD) in the elderly is an important public health issue due to increased proportion of this population many countries including Brazil. We aimed characterise pneumococci isolates adults >50 years with IPD, following introduction 10-valent conjugate vaccine (PCV10) as part National Childhood Immunisation Program for children ≤2 March 2010.Methodology. Between 2013 and 2015, were collected serotypes determined using multiplex PCR and/or Quellung...
This study aims to adapt a questionnaire about the knowledge, beliefs and behaviors regarding HPV related subjects into Brazilian Portuguese.National Survey.The instrument was translated Portuguese retranslated English separately. Experts assessed validity of content cross-cultural adaptation instrument. The administered 8580 male female adolescents young adults (aged between 16 25 years) who participated in National Survey Human Papillomavirus Prevalence (POP-Brazil). large-scale survey...
Clostridium (Clostridioides) difficile infection (CDI) is recognized worldwide as a public health concern, related mainly with hypervirulent strains. In Brazil there are few studies about molecular epidemiology of C. difficile, for this reason, we aimed to characterize isolates from large cohort study three different Brazilian states identify virulence and resistance genes, specifically genes metronidazole vancomycin resistance.All 153 fecal samples were submitted culture in CM0601 broth....
Coagulase-negative staphylococci (CoNS) are an important cause of nosocomial bacteremia, specially in patients with indwelling devices or those submitted to invasive medical procedures. The identification species and the accurate rapid detection methicillin resistance directly dependent on quality susceptibility tests used, either manual automated. objective this study was evaluate accuracy two automated systems MicroScan Vitek - CoNS determination methicillin, considering as gold standard...