A5004675827- Bacteriophages and microbial interactions
- Antibiotic Resistance in Bacteria
- Gut microbiota and health
- Microbial infections and disease research
- Antimicrobial Resistance in Staphylococcus
- Listeria monocytogenes in Food Safety
- Genomics and Phylogenetic Studies
- Biochemical and Structural Characterization
- Pharmaceutical and Antibiotic Environmental Impacts
- Bacterial biofilms and quorum sensing
- Bacterial Infections and Vaccines
- Clostridium difficile and Clostridium perfringens research
- Identification and Quantification in Food
- Mycobacterium research and diagnosis
- Complement system in diseases
- Enterobacteriaceae and Cronobacter Research
- Toxin Mechanisms and Immunotoxins
- Bacterial Genetics and Biotechnology
- Aquaculture disease management and microbiota
- Vibrio bacteria research studies
- S100 Proteins and Annexins
- Biosimilars and Bioanalytical Methods
- Genetic Associations and Epidemiology
- Bladder and Urothelial Cancer Treatments
- Salmonella and Campylobacter epidemiology
Canadian Food Inspection Agency
2020-2024
University of Toronto
2016-2020
Texas A&M Health Science Center
2011-2016
Texas A&M University
2016
York University
2016
Mitchell Institute
2013
Huazhong Agricultural University
2006-2011
State Key Laboratory of Agricultural Microbiology
2010
Members of a family collagen-binding microbial surface components recognizing adhesive matrix molecules (MSCRAMMs) from Gram-positive bacteria are established virulence factors in several infectious diseases models. Here, we report that these adhesins also can bind C1q and act as inhibitors the classical complement pathway. Molecular analyses Cna Staphylococcus aureus suggested this prototype MSCRAMM bound to collagenous domain interfered with interactions C1r C1q. As result, C1r2C1s2 was...
Inflammatory bowel disease patients have a greatly increased risk of developing colitis-associated colon cancer (CAC); however, the basis for inflammation-induced genetic damage requisite neoplasia is unclear. Using three models CAC, we find that sustained inflammation triggers 8-oxoguanine DNA lesions. Strikingly, antioxidants or iNOS inhibitors reduce and polyps in CAC models. Because mismatch repair (MMR) system repairs frequently defective colorectal (CRC), test whether mediates...
Actinobacillus pleuropneumoniae is the etiologic agent of porcine contagious pleuropneumonia, a cause considerable world wide economic losses in swine industry. We sequenced complete genome A. pleuropneumoniae, JL03, an isolate serotype 3 prevalent China. Its single chromosome 2,242,062 base pairs containing 2,097 predicted protein-coding sequences, six ribosomal rRNA operons, and 63 tRNA genes. Preliminary analysis genomic sequence functions encoded proteins not only confirmed present...
Thermostable nuclease is known to be an important pathogenic factor unique Staphylococcus aureus and it commonly presumed have had the same genetic origin. However, two ORFs in S. genomes were predicted encode nucleases. One encoded unnamed A (SNase) (termed nuc1), other a thermonuclease (TNase) named nuc nuc2). In order verify whether thermostable proteins are coexpressed aureus, nuc1 nuc2 genes cloned expressed Escherichia coli, both of recombinant showed activity toluidine blue-DNA assay....
ABSTRACT Coagulase (Coa) and Efb, secreted Staphylococcus aureus proteins, are important virulence factors in staphylococcal infections. Coa interacts with fibrinogen (Fg) induces the formation of fibrin(ogen) clots through activation prothrombin. Efb attracts Fg to bacterial surface forms a shield protect bacteria from phagocytic clearance. This communication describes use an array synthetic peptides identify variants linear binding motif present which responsible for activities these...
The staphylococcal nuclease, encoded by the nuc1 gene, is an important virulence factor of Staphylococcus aureus. However, physiological role nuclease has not been fully characterized. current study observed that biofilm development could be prevented in nuclease-producing strains S. aureus; however, when gene was knocked out, ability to form a significantly increased. Scanning electron and confocal scanning laser microscopy were used evaluate formation. Moreover, product, recombinant NUC1...
Genomic variations comprise a spectrum of alterations, ranging from single nucleotide polymorphisms (SNPs) to large-scale structural variants (SVs), which play crucial roles in bacterial evolution and species diversification. Accurately identifying SNPs SVs is beneficial for subsequent evolutionary epidemiological studies. This study presents VariantDetective (VD), novel, user-friendly, all-in-one pipeline combining SNP SV calling generate consensus genomic using multiple tools.
Abstract The global spread of antimicrobial resistance genes (ARGs) poses a significant threat to public health. While antibiotics effectively treat bacterial infections, they can also induce gut dysbiosis, the severity which varies depending on specific antibiotic treatment used. However, it remains unclear how dysbiosis affects mobility and dynamics ARGs. To address this, mice were pre-treated with streptomycin, ampicillin, or sulfamethazine, then orally inoculated Salmonella enterica...
Klebsiella michiganensis is a Gram-negative opportunistic pathogen that associated with many hospital-acquired infections in humans. Here, we report the complete genome sequence of K. strain isolated from Canadian wastewater treatment facility.
Abstract Accurate quantification of viable and non-viable bacteria is fundamental to most microbiological studies, thus, rapid simple techniques are required for this purpose. The current protocol describes a procedure using the QUANTOM Tx™ Microbial Cell Counter (Logos Biosciences Ltd., Korea), novel instrument quantitative measurement total microbial cells in solution, several bacterial species including Listeria monocytogenes , Escherichia coli Campylobacter jejuni Brucella species.
Ingestion of food- or waterborne antibiotic-resistant bacteria may lead to the dissemination antibiotic-resistance genes in gut microbiota and development bacterial infection, a significant threat animal public health. Food water be contaminated with multiple resistant bacteria, but models on gene transfer were mainly based single-strain infections. In this study, we investigated mobility 𝛽-lactam resistance following infection single- versus multi-strain under ampicillin treatment. We...
Objective. We sought to evaluate the immune responses a bivalent Haemophilus influenzae glycoconjugate vaccine against serotypes “a” (Hia) and “b” (Hib) in presence of preexisting immunity Hib. Methods. developed stochastic simulation model humoral response investigate antigenic challenge combined unimolecular vaccine. compared outcomes absence any with an already primed having specific memory B cells and/or anti-Hib antibodies. Results. The results show that Hib or carrier protein (CP) may...
Listeria monocytogenes is a Gram-positive, rod-shaped, non-spore-forming bacterium which an important foodborne bacterial pathogen for human worldwide with 20-30% mortality. Here, we report circular complete genome sequences of three strains isolated from the samples microgreens in Canada.
Dissemination of microorganisms with antimicrobial resistance genes (ARGs) through the food chain has been recognized as a growing public health concern worldwide. Biosolids, product wastewater treatment process, have used fertilizers in agriculture globally and also considered potential source pathogens ARGs for horizontal transfer across various environments. This study characterized antibiotic resistomes microbiota 24 biosolids samples collected from two Canadian waste water plants...
Raoultella planticola is a Gram-negative opportunistic bacterial pathogen associated with hospital-acquired infections in humans. Here, we report the complete genome sequence of one strain isolated from Canadian wastewater treatment facilities containing chromosome and four plasmids antimicrobial resistance (AMR) genes metal gene clusters.
Enteroaggregative
The global spread of antimicrobial resistance genes (ARGs) poses a significant threat to public health. While antibiotics effectively treat bacterial infections, they can also induce gut dysbiosis, the severity which varies depending on specific antibiotic treatment used. However, it remains unclear how dysbiosis affects mobility and dynamics ARGs. To address this, mice were pre-treated with streptomycin, ampicillin, or sulfamethazine, then orally inoculated
Ingestion of food- or waterborne antibiotic-resistant bacteria may lead to dissemination antibiotic resistance genes (ARGs) in the gut microbiota. The microbiota often suffers from various disturbances. It is not clear whether and how disturbed affect ARG mobility under treatments. For proof concept, presence absence streptomycin pre-treatment, mice were inoculated orally with a
ABSTRACT The sae operon is a key regulator in Staphylococcus aureus, which known as an important infective and toxigenic bacterial pathogen . For the exploration of virulence factors expressed secreted exoprotein fraction are being controlled by operon, relationship between locus exoproteins was investigated this study. homologous recombination vector pBT 2 Δ constructed deletion mutant strain successfully obtained. results showed that played role production thermonucleases other...
Listeria monocytogenes, a Gram-positive, rod-shaped, non-spore-forming bacterium, is an important foodborne bacterial pathogen for humans worldwide. Here, we report the complete genome sequence of Canadian monocytogenes strain with antimicrobial resistance (AMR) gene that was isolated from lettuce.