A5091889237- Salmonella and Campylobacter epidemiology
- Animal Nutrition and Physiology
- Food Safety and Hygiene
- Listeria monocytogenes in Food Safety
- Viral gastroenteritis research and epidemiology
- Antibiotic Resistance in Bacteria
- Livestock and Poultry Management
- Vibrio bacteria research studies
- Fecal contamination and water quality
- Pharmaceutical and Antibiotic Environmental Impacts
- Aquaculture disease management and microbiota
- Microbial infections and disease research
- Identification and Quantification in Food
- Survey Sampling and Estimation Techniques
- Escherichia coli research studies
- Agriculture, Soil, Plant Science
- Leptospirosis research and findings
- Bacterial Identification and Susceptibility Testing
- Meat and Animal Product Quality
- Antimicrobial Resistance in Staphylococcus
- Genomics and Phylogenetic Studies
- Clostridium difficile and Clostridium perfringens research
- Fisheries and Aquaculture Studies
- Plant Pathogenic Bacteria Studies
- Bacteriophages and microbial interactions
UNSW Sydney
2009-2024
Institute of Clinical Research
2024
The University of Sydney
2012-2024
Westmead Hospital
2024
In a structured survey of all major chicken-meat producers in Australia, we investigated the antimicrobial resistance (AMR) and genomic characteristics
The World Health Organisation has defined "highest priority critically important antimicrobials" (CIAs) as those requiring the greatest control during food production. Evidence demonstrating that restricted antimicrobial usage prevents emergence of resistance to CIA's amongst pathogenic and commensal organisms on a production system-wide scale would strengthen international efforts (AMR). Therefore, in designed survey all major chicken-meat producers Australia, we investigated phenotypic AMR...
The increasing resistance of bacteria to antimicrobials is a major threat public health. This study investigates the prevalence antimicrobial resistance, both phenotypic and genotypic, among Campylobacter isolates from Australian meat chickens in 2022, as follow up investigate trends since last national surveillance undertaken 2016. Isolates (n = 186) were obtained at slaughter 200 pooled cecal samples taken 1,000 chickens. majority C. jejuni (68.7%) coli (88.9%) susceptible all antibiotics...
To validate the effectiveness of a miniaturized most probable number method (mMPN) in enumerating Salmonella from poultry matrices.A MPN was developed, based on ISO 6579:2002 using modified semi-solid Rappaport-Vassiliadis media as sole selective medium. The validation mMPN shown to not differ significantly from, at 95% confidence level (Student's t-test P = 0·357) to, traditional 9-tube (tMPN) pure cultures ser. Typhimurium, Infantis, Montevideo, Muenster and subsp II 1,4,12,27:b:[e,n,x]...
In Australia, Salmonella serovar Typhimurium (S. Typhimurium) is the predominant zoonotic in humans and frequently isolated from layer hens. Vaccination against this has been previously shown to be effective broilers aim of current study was assess determine best vaccination strategy (live or inactivated) minimise caecal colonisation by S. Typhimurium.A long-term experiment (56 weeks) conducted on ISABROWN pullets using a commercial live aroA deleted mutant vaccine an autogenous inactivated...
Due to Australia's management of antimicrobial use in poultry, particularly the discontinued avoparcin for nearly 20 years, it is hypothesized that vancomycin-resistant enterococci associated with human disease are not derived from poultry isolates. This study evaluated resistance (AMR) five enterococcal species isolated Australian meat chickens, genomic features
An autologous killed trivalent vaccine (3×108 colony-forming units [CFU]), based on three Salmonella serovars (Typhimurium – serogroup B, Mbandaka C, and Orion E) prevalent in the flocks of Australian poultry companies, was developed using Salenvac® techniques. At 20 weeks, hens vaccinated at 12 17 weeks as well non-vaccinated were challenged (250 µl 107 CFU) with heterologous belonging to B Agona), C (Mbandaka Infantis) E (Orion Zanzibar). Overall, vaccination resulted a significant...
Aims: To evaluate a semi-automated repetitive extragenic palindromic sequence-based PCR (rep-PCR) system for the classification of Salmonella serotypes from Australian poultry. Methods and Results: Using DNA fingerprint library within DiversiLab® System, four separate databases were constructed (serogroup B, C, E Other). These contained 483 serologically confirmed (reference laboratory) isolates. A blinded set cultures (n = 155) typed by rep-PCR, matched against internal compared with...
Controlling the use of most critically important antimicrobials (CIAs) in food animals has been identified as one key measures required to curb transmission antimicrobial resistant bacteria from humans. Expanding evidence demonstrating effectiveness restricting CIA usage for preventing emergence resistance drugs amongst commensal organisms animal production would do much strengthen international efforts control (AMR). As Australia strict controls on layer hens, and internationally...
Members of the genus Salmonella represent a significant public health concern and also colonizer commercial poultry. Therefore, early detection management colonized broiler breeders their progeny is essential. There have been numerous methods for farm-based detection, with gauze-based drag swabs being most commonly used. In present study, wet (boiled water, buffered peptone water double-strength skin milk) tampon was compared gauze to determine recovery rate (10(2) colony-forming units/swab)...
Campylobacter hepaticus , the causative agent of Spotty Liver Disease (SLD) is an important disease in cage-free egg producing chickens causing mortality and production drops. C . a slow growing easily overgrown by fecal bacteria. It currently only reliably isolatable from bile samples. A selective media for isolation feces or environment would assist diagnosis impact assessment. Growth five Australian isolates was studied using Horse blood agar (HBA), sheep (SBA), Bolton, Preston Brain...
Contamination of poultry products by Salmonella enterica serovar Typhimurium (STm) is a major cause foodborne infections and outbreaks. This study aimed to assess the diversity antimicrobial resistance (AMR) carriage STm in three chicken processing plants using genomic sequencing. It also investigate whether any particular strain types were associated with cases human illness. Multilevel genome typing (MGT) was used analyze 379 isolates from processed carcasses. The sequence (STs) increased...
Abstract Salmonella enterica serovar Abortusovis is a ovine-adapted pathogen that causes spontaneous abortion. was reported in poultry 2009 and has since been human infections New South Wales, Australia. Phylogenomic analysis revealed clade of 51 closely related isolates from Australia originating 2004. That genetically distinct ovine-associated isolates. The widespread Wales production facilities but only responsible for sporadic infections. Some known virulence factors associated with were...
Abstract Controlling the use of most critically important antimicrobials (CIAs) in food animals has been identified as one key measures required to curb transmission antimicrobial resistant bacteria from humans. Expanding evidence demonstrating effectiveness restricting CIA usage for preventing emergence resistance drugs amongst commensal organisms animal production would do much strengthen international efforts control (AMR). As Australia strict controls on layer hens, and internationally...
Campylobacter spp. contaminated poultry products are strongly associated with foodborne illnesses worldwide. Development of effective management strategies to reduce contamination by requires an improved understanding the numerous factors that drive these processes. Currently, chicken farms using more free-range meat production systems in response consumer preferences. However, colonization has rarely been investigated on broiler farms. The present study temporal and environmental...
Enteropathogens, namely Salmonella and Campylobacter, are a concern in global public health have been attributed numerous risk assessments to poultry source. During the last decade large body of research addressing this problem has published. The literature reviewed contains review articles on certain aspects production chain, however past there hasn’t through farm fork, poultry. This review, pool 514 were selected for relevance via systematic screening process (from >7500...