Pigs have been recognised as a reservoir of livestock associated methicillin-resistant Staphylococcus aureus (LA-MRSA) in Europe, Asia and North America. However, little is known about the presence distribution MRSA Australian pig population industry. This study describes presence, molecular characteristics human adapted CA-MRSA ST93 isolated from pigs, people, environment within piggery. Isolates were subjected to antibiotic susceptibility testing, DNA microarray, whole genome sequencing,...

In a structured survey of all major chicken-meat producers in Australia, we investigated the antimicrobial resistance (AMR) and genomic characteristics

The World Health Organisation has defined "highest priority critically important antimicrobials" (CIAs) as those requiring the greatest control during food production. Evidence demonstrating that restricted antimicrobial usage prevents emergence of resistance to CIA's amongst pathogenic and commensal organisms on a production system-wide scale would strengthen international efforts (AMR). Therefore, in designed survey all major chicken-meat producers Australia, we investigated phenotypic AMR...

Infections due to methicillin-resistant Staphylococcus aureus (MRSA) are present worldwide and represent a major public health concern. The capability of PCR followed by high-resolution melt (HRM) curve analysis for the detection community-associated livestock-associated MRSA strains identification staphylococcal protein A ( spa ) locus was evaluated in 74 samples which were isolated from environment, humans, pigs on single piggery. PCR-HRM identified four types among differentiated accordingly.

Campylobacter is a major cause of gastroenteritis worldwide, with broiler meat accounting for most illnesses. Antimicrobial intervention recommended in severe cases campylobacteriosis. The emergence antimicrobial resistance (AMR) concerning food safety challenge, and monitoring the trends AMR vital better risk assessment. This study aimed to characterize phenotypic profiles molecular markers virulence prevalent species contaminating chilled chicken carcasses sampled from supermarkets United...

Aflatoxin-contaminated feed cause mortality, suppression of the immune system, reduced growth rates and losses in efficiency.This research study was planned to investigate immunomodulatory promoting effect milk thistle as additive against aflatoxin B 1 broiler chicks at NWFP Agricultural University Peshawar, Pakistan.Two hundred forty (240) day old broilers were randomly assigned into four major groups AfF, free feed; Aflatoxin present levels 80-520 μg/kg remaining three groups.Aflatoxin...

Due to Australia's management of antimicrobial use in poultry, particularly the discontinued avoparcin for nearly 20 years, it is hypothesized that vancomycin-resistant enterococci associated with human disease are not derived from poultry isolates. This study evaluated resistance (AMR) five enterococcal species isolated Australian meat chickens, genomic features

Background and Aim: Despite the endemicity of Japanese encephalitis virus (JEV) in humans animals Province Bali, Indonesia, there is little data on whether seroconversion to occurs pigs, JEV genotypes circulating, it’s potential mosquito vectors area. The aims this study were (i) Determine infection Balinese pigs before reaching their sexual maturity, (ii) identify circulating JEV, (iii) at sites urban peri-urban areas Bali. Materials Methods: Sixteen 1-week-old Landrace piglets from two...

Over the last three decades, hospital adapted clonal complex (CC) 17 strains of Enterococcus faecium have acquired and exchanged antimicrobial resistance genes leading to widespread clinically important antimicrobials globally. In Australia, a high prevalence vancomycin has been reported in E. decade.In this study, we determined phylogenetic relationship genetic characteristics collected from hospitalized patients with blood stream infections throughout Australia 2015 2017 using throughput...

Enterococci are ubiquitous opportunistic pathogens that have become a major public health issue globally. The increasing prevalence of antimicrobial resistance in hospital-adapted enterococci had been thought to originate from livestock. However, this association between livestock and is currently unclear. This study investigates the susceptibilities isolated pig cecal samples compares genomic characteristics Enterococcus faecium pigs those isolates meat chickens human sepsis cases. From 200...

This observational study aimed to determine MRSA prevalence using strain-specific real-time PCR at the pig level, stratified by age groupings, within a enterprise. A total of 658 samples were collected from individual pigs (n = 618) and piggery environment 40), distributed amongst five different groups. Presumptive isolates confirmed presence mecA, MALDI-TOF was performed for species verification. All tested against 18 antimicrobials. isolated 75.2% (95% CI 71.8-78.6) pigs, 71% this source...

A key component to control of antimicrobial resistance (AMR) is the surveillance food animals. Currently, national programmes test only limited isolates per animal species year, an approach tacitly assuming that heterogeneity AMR across populations negligible. If latter assumption incorrect then risk humans from in chain underestimated.To demonstrate extent phenotypic and genetic Escherichia coli swine assess need for improved protocols animals.Eight E. were obtained each 10 pigs on farms....

Dairy cows can be reservoirs of foodborne pathogens such as Salmonella that pose serious public health risks to humans. The study was designed examine the molecular epidemiology and antimicrobial resistance profiles isolates from dairy heifer calves adult lactating in pasture-based system Australia. A total 838 animals (328 510 cows) 22 farms were sampled. Overall, 54 recovered (calves 28/328 26/510). herd-level prevalence 50% (95% confidence interval: 31%-69%) recorded. Within-herd for...

Background The occurrence of livestock-associated (LA) MRSA (ST398) in pig herds has emerged as a threat to occupational safety many parts the world. Recently, an outbreak skin lesions due occurred workers at farm regional Australia and both humans pigs were shown have high prevalence carriage either human-strain ST93 or porcine strain ST398. This study closely scrutinises this determine factors associated with amongst workers. Methods Information on potential risk was collected from...

This study investigated the validity of DNA-marker based test to determine susceptibility ETEC-F4 diarrhoea by comparing results two DNA sequencing techniques in weaner pigs following experimental infection with F4 enterotoxigenic Escherichia coli (ETEC-F4). The effects diet and genetic were assessed measuring incidence piglet post-weaning (PWD), faecal E. shedding index. A marker-based targeting mucin 4 gene (MUC4) that encodes fimbria receptor identified as either fully susceptible (SS),...

This study aimed to determine the bacterial isolates associated with postpartum endometritis among dairy cows in Western Australia and their antimicrobial susceptibility profiles. A cross-sectional was conducted between June-October 2020. Endometritis defined as evidence of mucopurulent purulent vaginal discharge 60-100 days postpartum. Vaginal samples were obtained, cultured, identified tested for susceptibility. total 118 grown from 46 animals, representing 36 species. The bacteria...

Australia relies on periodic antimicrobial resistance (AMR) surveys to determine trends and changes in AMR animal production systems. This study is a follow-up survey of Escherichia coli from healthy cattle at slaughter conducted 2013, which provided baseline data prevalence across groups practices. In this study, 591 beef cattle, 194 dairy 216 veal calf fecal samples were collected 25 processing establishments Australia, representing approximately 77% total export volume. A 969...