A5044946451- Lipid Membrane Structure and Behavior
- Protein Structure and Dynamics
- ATP Synthase and ATPases Research
- RNA and protein synthesis mechanisms
- Cellular Mechanics and Interactions
- Bacteriophages and microbial interactions
- Advanced Materials and Mechanics
- Alkaline Phosphatase Research Studies
- Influenza Virus Research Studies
- Bone health and treatments
- Ion Channels and Receptors
- Herbal Medicine Research Studies
- Ion Transport and Channel Regulation
- Advanced NMR Techniques and Applications
- Photosynthetic Processes and Mechanisms
- Force Microscopy Techniques and Applications
- Microtubule and mitosis dynamics
- Advanced Fluorescence Microscopy Techniques
- Advanced MRI Techniques and Applications
- Mitochondrial Function and Pathology
- Membrane-based Ion Separation Techniques
- Essential Oils and Antimicrobial Activity
- Ion channel regulation and function
- Antifungal resistance and susceptibility
University of California, San Francisco
2015-2024
Madonna University
2022
The M2 proton channel aids in the exit of mature influenza viral particles from host plasma membrane through its ability to stabilize regions high negative Gaussian curvature (NGC) that occur at neck budding virions. channels are homo-tetramers contain a cytoplasm-facing amphipathic helix (AH) is necessary and sufficient for NGC generation; however, constructs containing transmembrane spanning helix, which facilitates tetramerization, exhibit enhanced generation. Here, we used all-atom...
Abstract Sodium-dependent glucose transporters (SGLTs) couple a downhill Na + ion gradient to actively transport sugars. Here, we investigate the impact of membrane potential on vSGLT structure and function using sugar uptake assays, double electron-electron resonance (DEER), electrostatic calculations, kinetic modeling. Negative potentials, as present in all cell types, shift conformational equilibrium towards an outward-facing conformation, leading increased rates. Electrostatic...
The lumenal pH of an organelle is one its defining characteristics and central to biological function. Experiments have elucidated many the key regulatory elements how they vary from compartment-to-compartment, continuum mathematical models played important role in understanding these (proton pumps, counter-ion fluxes, membrane potential, buffering capacity, etc.) work together achieve specific setpoints. While proven successful describing ion regulation at cellular length scale, it unknown...
Continuum or hybrid modeling of bilayer membrane morphological dynamics induced by embedded proteins necessitates the identification protein-membrane interfaces and coupling deformations two surfaces. In this article we developed (i) a minimal total geodesic curvature model to describe these interfaces, (ii) numerical one-one mapping between surface through conformal each common middle annulus. Our work provides first computational tractable approach for determining proteins. The allows...
Abstract The M2 proton channel aids in the exit of mature influenza viral particles from host plasma membrane through its ability to stabilize regions high negative gaussian curvature (NGC) that occur at neck budding virions. channels are homo-tetramers contain a cytoplasm-facing amphipathic helix (AH) is necessary and sufficient for NGC generation; however, constructs containing transmembrane spanning helix, which facilitates tetramerization, exhibit enhanced generation. Here we used...