A5001095773- Neuroscience and Neuropharmacology Research
- Lipid Membrane Structure and Behavior
- Cellular transport and secretion
- Neuroinflammation and Neurodegeneration Mechanisms
- Tryptophan and brain disorders
- Adenosine and Purinergic Signaling
- Mitochondrial Function and Pathology
- RNA Interference and Gene Delivery
- Ion channel regulation and function
- Biochemical Analysis and Sensing Techniques
- Parkinson's Disease Mechanisms and Treatments
- Metabolism and Genetic Disorders
- Stress Responses and Cortisol
- ATP Synthase and ATPases Research
- Erythrocyte Function and Pathophysiology
- Calpain Protease Function and Regulation
- Epilepsy research and treatment
- Alzheimer's disease research and treatments
- RNA regulation and disease
- Musculoskeletal pain and rehabilitation
- Supramolecular Self-Assembly in Materials
- Biochemical effects in animals
- Pharmacological Receptor Mechanisms and Effects
- Workaholism, burnout, and well-being
- Lysosomal Storage Disorders Research
Max Planck Institute for Multidisciplinary Sciences
2021-2024
Max Planck Institute for Biophysical Chemistry
2015-2024
Tissue Dynamics (Israel)
2021
University of Göttingen
2021
Max Planck Society
2015-2017
Universidade Federal do Rio Grande do Sul
2004-2017
Neuronal transmission relies on the regulated secretion of neurotransmitters, which are packed in synaptic vesicles (SVs). Hundreds SVs accumulate at boutons. Despite being held together, highly mobile, so that they can be recruited to plasma membrane for their rapid release during neuronal activity. However, how such confinement corroborates with motility remains unclear. To bridge this gap, we employ ultrafast single-molecule tracking (SMT) reconstituted system native and living neurons....
Neurotransmission relies on the tight spatial and temporal regulation of synaptic vesicle (SV) cycle. Nerve terminals contain hundreds SVs that form clusters. These clusters represent a distinct liquid phase in which one component are other synapsin 1, highly abundant protein. Another major family disordered proteins at presynapse includes synucleins, most notably α-synuclein. The precise physiological role α-synuclein physiology remains elusive, albeit its has been implicated nearly all...
Vesicular transporters (VTs) define the type of neurotransmitter that synaptic vesicles (SVs) store and release. While certain mammalian neurons release multiple transmitters, it is not clear whether occurs from same or distinct vesicle pools at synapse. Using quantitative single-vesicle imaging, we show a vast majority SVs in rodent brain contain only one VT, indicating specificity for single neurotransmitter. Interestingly, containing dual are highly diverse (27 types) but small proportion...
Abstract Synaptic vesicles are storage organelles for neurotransmitters. They pass through a trafficking cycle and fuse with the pre-synaptic membrane when an action potential arrives at nerve terminal. While molecular components biophysical parameters of synaptic have been determined, our knowledge on protein interactions in their membranes is limited. Here, we apply cross-linking mass spectrometry to study vesicle proteins unbiased approach without need specific antibodies or...
In neurons, autophagosome biogenesis occurs mainly in distal axons, followed by maturation during retrograde transport. Autophagosomal growth depends on the supply of membrane lipids which requires small vesicles containing ATG9, a lipid scramblase essential for macroautophagy/autophagy. Here, we show that ATG9-containing are enriched synapses and resemble synaptic size density. The proteome immuno-isolated from nerve terminals showed conspicuously low levels trafficking proteins except...
Abstract We study the formation of vesicle condensates induced by protein synapsin, as a cell-free model system mimicking pool in synapse. The can be considered an example liquid–liquid phase separation (LLPS) biomolecular fluids, where one is complex fluid itself consisting vesicles and network. address pertinent question why LLPS self-limiting stops at certain size, i.e., macroscopic prevented. Using fluorescence light microscopy, we observe different morphologies (aggregates) depending on...
Essential omega-3 polyunsaturated fatty acids (omega3) are crucial to brain development and function, being relevant for behavioral performance. In the present study we examined influence of dietary omega3 in glutamatergic system on behavior parameters rats. Female rats received isocaloric diets, either with (omega3 group) or a deficient diet (D group). ontogeny experiments their litters, hippocampal immunocontent ionotropic NMDA AMPA receptors subunits (NR2 A\B GluR1, respectively) alpha...
Abstract Regulated exocytosis of synaptic vesicles is substantially faster than endocrine dense core despite similar molecular machineries. The reasons for this difference are unknown and could be due to different regulatory proteins, spatial arrangements, vesicle sizes, or other factors. To address these questions, we take a reconstitution approach compare regulated SNARE-mediated fusion purified chromaffin insulin using single vesicle-supported membrane assay. In all cases, Munc18...
Summary: Purpose: N ‐methyl d ‐aspartate (NMDA) preconditioning has been used to prevent cellular death induced by glutamate or NMDA in cultured neurons. Quinolinic acid (QA)‐induced seizures are average receptors–evoked neurotoxicity animal models. The purpose of this study was investigate the potential neuroprotective effects against QA‐induced and hippocampal damage vivo. Methods: Mice were pretreated with nonconvulsant doses for different times before i.c.v. QA infusion observed...
Major depressive disorder (MDD) is a neuropsychiatric disease that associated with profound disturbances in affected individuals. Elucidating the pathophysiology of MDD has been frustratingly slow, especially concerning neurochemical events and brain regions progression. Thus, we evaluated time-course (up to 8 weeks) behavioral biochemical effects mice underwent bilateral olfactory bulbectomy (OBX), which used modeling depressive-like behavior rodents. Similar symptoms patients MDD, OBX...
JM-20 (3-ethoxycarbonyl-2-methyl-4-(2-nitrophenyl)-4,11-dihydro-1H-pyrido[2,3-b][1,5]benzodiazepine) is a novel benzodiazepine dihydropyridine hybrid molecule, which has been shown to be neuroprotective agent in brain disorders involving glutamate receptors. However, the effect of on functionality glutamatergic system not investigated. In this study, by using different vitro preparations, we investigated effects (i) rat synaptic vesicles (L-[(3)H]-glutamate uptake, proton gradient built-up...
Vesicular glutamate transporters (VGLUTs) fill synaptic vesicles with and are thus essential for glutamatergic neurotransmission. However, VGLUTs were originally discovered as members of a transporter subfamily specific inorganic phosphate (Pi). It is still unclear how accommodate transport coupled to an electrochemical proton gradient ΔμH+ inversely directed Pi the Na+ membrane potential. Using both functional reconstitution heterologous expression, we show that VGLUT transports using...
Major depressive disorder (MDD) leads to pervasive changes in the health of afflicted patients. Despite advances understanding MDD and its treatment, profound innovation is needed develop fast-onset antidepressants with higher effectiveness. When acutely administered, endogenous nucleoside guanosine (GUO) shows antidepressant-like effects several mouse models, including olfactory bulbectomy (OBX) rodent model. OBX advocated possess translational value be suitable assess time course...
Vesicular glutamate transporters (VGLUTs) fill synaptic vesicles with glutamate. VGLUTs were originally identified as sodium-dependent of inorganic phosphate (Pi), but the physiological relevance this activity remains unclear. Heterologous expression all three greatly augments intracellular Pi levels. Using neuronal models, we show that translocation to plasma membrane during exocytosis results in highly increased uptake. VGLUT-mediated influx is counteracted by efflux. Synaptosomes prepared...