A5064461641- Medicinal Plants and Neuroprotection
- Neuroscience and Neuropharmacology Research
- Cholinesterase and Neurodegenerative Diseases
- Neuroinflammation and Neurodegeneration Mechanisms
- Alzheimer's disease research and treatments
- Parkinson's Disease Mechanisms and Treatments
- Neurological Disease Mechanisms and Treatments
- Natural product bioactivities and synthesis
- Natural Compound Pharmacology Studies
- Herbal Medicine Research Studies
- Biological and pharmacological studies of plants
- Essential Oils and Antimicrobial Activity
- Bioactive natural compounds
- Antioxidant Activity and Oxidative Stress
- Medical research and treatments
- Biochemical Acid Research Studies
- Psidium guajava Extracts and Applications
- Natural Antidiabetic Agents Studies
- Aluminum toxicity and tolerance in plants and animals
- Traumatic Brain Injury and Neurovascular Disturbances
- Free Radicals and Antioxidants
- Phytochemical Studies and Bioactivities
- Receptor Mechanisms and Signaling
- Natural Products and Biological Research
- Morinda citrifolia extract uses
Centro de Aplicaciones Tecnológicas y Desarrollo Nuclear
2016-2025
Centro de Investigación y Desarrollo de medicamentos
2015-2024
Centro de Investigación y Desarrollo
2007-2024
Centre d'Innovació i Desenvolupament Empresarial
2021-2024
Centro de Información y Desarrollo de la Mujer
2023-2024
Universidade Federal de Santa Maria
2024
Universidade Católica de Pelotas
2023
University of Havana
2018
University of Antofagasta
2016
Cuban Neuroscience Center
2013
Guttiferone-A (GA) is a natural occurring polyisoprenylated benzophenone with cytotoxic action in vitro and anti-tumor rodent models. We addressed potential involvement of mitochondria GA toxicity (1-25 μM) toward cancer cells by employing both hepatic carcinoma (HepG2) succinate-energized mitochondria, isolated from rat liver. In HepG2 decreased viability, dissipated mitochondrial membrane potential, depleted ATP increased reactive oxygen species (ROS) levels. rat-liver promoted fluidity...
Because mitochondrial oxidative stress and impairment are important mediators of neuronal damage in neurodegenerative diseases brain ischemia/reperfusion, the present study, we evaluated antioxidant mitoprotective effect a new promising neuroprotective molecule, JM-20, mitochondria synaptosomes isolated from rat brains. JM-20 inhibited succinate-mediated H₂O₂ generation both incubated depolarized (high K(+)) medium at extremely low micromolar concentration with identical IC₅₀ values 0.91 μM....
Scopolamine (SCO) administration to rats induces molecular features of AD and other dementias, including impaired cognition, increased oxidative stress, imbalanced cholinergic transmission. Although mitochondrial dysfunction is involved in different types its role cognitive impairment induced by SCO has not been well elucidated. The aim this work was evaluate the vivo effect on brain parameters explore neurotoxic mechanisms action.Saline (Control) or (1 mg/kg) administered intraperitoneally...
The ischemic stroke cascade is composed of several pathophysiological events, providing multiple targets for pharmacological intervention. JM-20 (3-ethoxycarbonyl-2-methyl-4-(2-nitrophenyl)-4,11-dihydro-1H-pyrido[2,3-b][1,5]benzodiazepine) a novel hybrid molecule, in which benzodiazepine portion covalently linked to dihydropyridine ring, forming new chemical entity with potential multisite neuroprotective activity. In the present study, prevented PC-12 cell death induced either by glutamate,...
AbstractObjectives: The aim of this study was to investigate the effects oral administration a novel benzodiazepine derivative, JM-20, on neurological behavior different rodent models, focusing GABAergic effect. We have also investigated acute toxicity JM-20 in mice.Methods: Mice or rats received at 2, 4, 8, and 10 mg/kg evaluate sedative/hypnotic, anxiolytic, anticonvulsant effects, as well influence stereotyped induced by amphetamine. Diazepam (DZP) used positive control. In addition, mice...
JM-20 (3-ethoxycarbonyl-2-methyl-4-(2-nitrophenyl)-4,11-dihydro-1H-pyrido[2,3-b][1,5]benzodiazepine) is a novel benzodiazepine dihydropyridine hybrid molecule, which has been shown to be neuroprotective agent in brain disorders involving glutamate receptors. However, the effect of on functionality glutamatergic system not investigated. In this study, by using different vitro preparations, we investigated effects (i) rat synaptic vesicles (L-[(3)H]-glutamate uptake, proton gradient built-up...
JM-20, a novel hybrid synthetic molecule, has been reported to have antioxidant, mitoprotective, anti-excitotoxic, anti-apoptotic and anti-inflammatory properties. However, the neuroprotective effect of JM-20 against memory impairment in preclinical AD-like models not analyzed. The aim this study was evaluate potential neuroprotection that preserves essential process from cholinergic dysfunction other molecular damages.The effects on scopolamine (1 mg/kg)-induced cognitive disorders were...
Clusianone is a member of the polycyclic polyprenylated acylphloroglucinol family natural products; its cytotoxic mechanism unknown. structural isomer nemorosone, which mitochondrial uncoupler and well-known anti-cancer agent; thus, we addressed clusianone action at mitochondria potential effects on cancer cells. In HepG2 hepatocarcinoma cell line, induced membrane dissipation, ATP depletion phosphatidyl serine externalization; this later event indicative apoptosis induction. isolated from...
Three main types of Cuban propolis directly related to their secondary metabolite composition have been identified: brown, red and yellow propolis; the former is majoritarian characterized by presence nemorosone. In this study, brown extracts were found cytotoxic against HepG2 cells primary rat hepatocytes, in close association with nemorosone contents. mitochondria isolated from liver displayed uncoupling activity, which was demonstrated increase succinate-supported state 4 respiration...
Amburana cearensis (Allemao) A.C.Sm. is a medicinal plant of the Brazilian Caatinga reported to present antioxidant and anti-inflammatory activity. This study aimed evaluate neuroprotective effect extracts obtained from seeds A. in primary cultures cerebellar cells subjected excitotoxicity induced by glutamate brain mitochondria submitted oxidative stress.and methods: Primary were treated with ethanol (ETAC), hexane (EHAC), dichloromethane (EDAC) ethyl acetate (EAAC) A.cearensis (10µM)....