A5039999854- Electrospun Nanofibers in Biomedical Applications
- Nerve injury and regeneration
- Tissue Engineering and Regenerative Medicine
- Spinal Cord Injury Research
- Advanced Sensor and Energy Harvesting Materials
- RNA Interference and Gene Delivery
- Additive Manufacturing and 3D Printing Technologies
- Wound Healing and Treatments
- Nerve Injury and Rehabilitation
- Silk-based biomaterials and applications
- biodegradable polymer synthesis and properties
- Youth Development and Social Support
- Tendon Structure and Treatment
- Graphene and Nanomaterials Applications
- Aortic Disease and Treatment Approaches
- Virus-based gene therapy research
- Botulinum Toxin and Related Neurological Disorders
- Conducting polymers and applications
- Higher Education Research Studies
- Evaluation of Teaching Practices
- Stroke Rehabilitation and Recovery
- Burn Injury Management and Outcomes
- Bone Tissue Engineering Materials
- 3D Printing in Biomedical Research
- Photopolymerization techniques and applications
Cornell University
2020-2024
Hollister (United States)
2021-2024
Rensselaer Polytechnic Institute
2015-2020
Nanofiber Solutions (United States)
2018
There is currently no cure for individuals with spinal cord injury (SCI). While many promising approaches are being tested in clinical trials, the complexity of SCI limits several these from aiding complete functional recovery. Several different categories biomaterials investigated their ability to guide axonal regeneration, deliver proteins or small molecules locally, improve viability transplanted stem cells. The purpose this study provide a brief overview SCI, present biomaterial...
Three aligned, electrospun fiber scaffolds with unique surface features were created from poly-L-lactic acid (PLLA). Fibers without nanotopography (smooth fibers), fibers divots (shallow pits), and pits (deeper pits) fabricated, alignment, diameter, density characterized using scanning electron microscopy (SEM). Whole dorsal root ganglia (DRG) isolated rats placed onto uncoated or coated laminin. On fibers, neurite outgrowth was restricted by displaying divoted pitted when compared to on...
Digital light processing (DLP)-based three-dimensional (3D) printing offers large improvements in fabrication throughput and spatial resolution when compared to various other additive manufacturing techniques. Both properties are highly desirable fabricating biomaterial scaffolds that require design precision. Poly(glycerol sebacate) acrylate (PGSA) is a degradable, biocompatible, photocurable elastomer. In this work, PGSA ink was developed for DLP 3D of porous tubular structures. Ink...
Electrospinning is a robust material fabrication method allowing for fine control of mechanical, chemical, and functional properties in scaffold manufacturing. Electrospun fiber scaffolds have gained prominence their potential variety applications such as tissue engineering textile manufacturing, yet none assessed the impact solvent retention fibers on scaffold's mechanical properties. In this study, we hypothesized that retained electrospinning acts plasticizer, gradual evaporation, by...
Aligned, electrospun fiber scaffolds provide topographical guidance for regenerating neurons and glia after central nervous system injury. To date, no study has explored how surface nanotopography affects astrocyte response to fibrous scaffolds. Astrocytes play important roles in the glial scar, blood brain barrier, maintaining homeostasis system. In this study, poly L-lactic acid fibers were engineered with smooth, pitted, or divoted nanotopography. Cortical spinal cord primary rat...
Abstract Central nervous system (CNS) injuries persist for years, and currently there are no therapeutics that can address the complex injury cascade develops over this time-scale. 17β-estradiol (E2) has broad tropism within CNS, targeting inducing beneficial phenotypic changes in myriad cells following injury. To unmet need vastly prolonged E2 release, we report first-generation poly(pro-E2) biomaterial scaffolds release at nanomolar concentrations course of 1–10 years via slow hydrolysis...
Currently, it is unknown how the mechanical properties of electrospun fibers, and presentation surface nanotopography influence macrophage gene expression protein production. By further elucidating specific fiber (mechanical or properties) alter behavior, may be possible to create scaffolds capable initiating unique cellular tissue responses. In this study, we determined elastic modulus rigidity fibers with varying topographies created by finely controlling humidity including a non-solvent...
Multiple additive manufacturing techniques have been developed in recent years to produce structures with tunable physical, chemical, and mechanical properties defined architecture. Solution electrospinning, although an older more established technique, normally cannot achieve the pattern resolution tunability of these newer techniques. In this study, we present solution electrowriting as a method fibrous conduits from various polymers patterns, dimensions, scaffold porosity. We demonstrate...
Macrophages are immune cells involved in wound healing and tissue regeneration; however, the sustained presence of proinflammatory macrophages sites impairs healing. In this study, we shifted peritoneal macrophage polarization away from a (M1) phenotype through exposure to stabilized interleukin-4 (IL-4) poly(lactic-co-glycolic acid) films combination with topographical guidance electrospun poly-l-lactic acid fibers. To our knowledge, was first study stabilize IL-4 bovine serum albumin (BSA)...
Researchers are investigating the use of biomaterials with aligned guidance cues, like those provided by electrospun fibers, to facilitate axonal growth across critical-length peripheral nerve defects. To enhance regenerative outcomes further, these fibers can be designed provide local, sustained release therapeutics. The drug fingolimod improved regeneration in preclinical rodent models stimulating a pro-regenerative Schwann cell phenotype and growth. However, systemic delivery for repair...
Electrospun poly-l-lactic acid (PLLA) fiber scaffolds are used to direct axonal extension in neural engineering models. We aimed improve the efficacy of these fibers promoting neurite outgrowth by altering surface topography and reducing elastic modulus through incorporation a compatibilized blend, acid-poly(pentadecalactone) (PLLA–PPDL) into solution prior electrospinning. PLLA+PLLA–PPDL had larger diameter, increased nanotopography, lower glass transition temperature than PLLA but similar...
Increasing the numbers of black, latino and native youth in STEM careers is both an important way to reduce poverty low income communities, a contribution diversity thought experience that drives research. But underrepresented are often alienated from STEM. Two new forms social capital have been identified can be combined create learning environment which students researchers meet explore area shared interest. Experimental refers intrinsic motivation develop when they learn inquiry...
The hydrophobicity of electrospun poly-l-lactic acid (PLLA) fibers hinders their integration with surrounding tissue for a variety applications. In this study, we increased PLLA fiber hydrophilicity by incorporating the natural surfactant, lactonic sophorolipid (LSL). PLLA+LSL had similar morphology but significantly greater surface wettability, which suggested LSL accumulation on surface. Differential scanning calorimetry results also that was phase separated from PLLA. Despite altered...
Abstract Objective . Macrophages and astrocytes play a crucial role in the aftermath of traumatic spinal cord injury (SCI). Infiltrating macrophages adopt pro-inflammatory phenotype while resident neurotoxic at site, both which contribute to neuronal death inhibit axonal regeneration. The cytokine interleukin-4 (IL-4) has shown significant promise preclinical models SCI by alleviating macrophage-mediated inflammation promoting functional recovery. However, its effect on reactive remains be...
In an attempt to increase the rate of neurite extension from E9 chick embryo dorsal root ganglion (DRG) on electrospun fiber scaffolds we experimented with two drugs that have been used previously affect neuronal growth. Blebbistatin and paclitaxel were added DRG cultured fibers in along fibrous scaffolds. increased length neurites extending explant comparison drug-free control group. contrast, completely eliminated all when compared control. A third treatment group which combined both also...
We hypothesized that fiber diameter within an electrospun scaffold would affect astrocyte elongation and maturation. To examine the relationship, astrocytes were seeded on 400nm 800nm PLLA fibers. Immunohistochemistry was used to monitor cell morphology, GLT-1 expression over first 96 hours after seeding. The results indicate extended along primary axis of fibers more rapidly than half size. While expressed diffusely throughout surface astrocytes, elongated processes exhibited dense regions...