The aim of this work was to (i) determine the chemical composition essential oils six spices widely cultivated in Egypt (Origanum syriacum, Majorana hortensis, Rosmarinus officinalis, Cymbopogon citratus, Thymus vulgaris, and Artemisia annua); (ii) antioxidant activity Egyptian by means five different tests; (iii) effectiveness these on inhibition Listeria innocua CECT 910. There is a great variability obtained from aromatic plants. Overall, thyme (highest percentage DPPH radical: 89.40%)...

Mastitis is one of the most vital noteworthy monetary risks to dairy ranchers and affects reproductive performance in cattle. However, subclinical mastitis (SCM) negatively milk quality quantity associated with economic losses as clinical mastitis. It recognizable only by additional testing. Somatic cell count (SCC) currently used worldwide for screening intramammary infection (IMI) infections. somatic cells (SC) are affected numerous factors not always correlate udder. Therefore, aim...

Listeria monocytogenes is considered one of the most important food-borne pathogens transmitted to humans via contaminated food. The aim present study was demonstrate importance L. as a pathogen.A total 340 samples were collected from different localities in El Giza Governorate, Egypt, check occurrence that area. comprised 250 food samples, 40 swabs refrigerators, and 50 stool specimens diarrheic children. isolated examined according International Organization for Standardization. isolates...

Abstract Klebsiella pneumoniae is an opportunistic pathogen responsible for various infections in humans and animals. It known its resistance to multiple antibiotics, particularly through the production of Extended-Spectrum Beta-Lactamases (ESBLs), ability form biofilms that further complicate treatment. This study aimed isolate identify K. from animal environmental samples assess commercial disinfectants’ effectiveness against isolates exhibiting ESBL-mediated biofilm-forming poultry equine...

Lumpy skin disease (LSD), is a highly infectious viral of cattle, caused by LSD virus (LSDV) which belongs to the genus Capripoxvirus family Poxviridae. In summer 2017, lesions suggestive were observed in cattle at several governorates Egypt. This study aimed detect LSDV specimens using rapid serological and molecular diagnostic assays.A total 46 biopsies uncoagulated blood samples collected from with clinical signs, as well 290 coagulated whole without lesion different Egypt during 2017....

Viral infection is one of the most important life-threatening animals health.The emergence new viral disease increases demand for therapeutic drugs.Current antiviral drugs have certain limitations treating infection.The objective present study was aimed to use plant extracts olive leaves and natural honey green synthesis zinc oxide (ZnONPs) silver nanoparticles (AgNPs) investigate potential activities against bovine herpesvirus-1(BoHV-1) in vitro vivo.The formation NPs confirmed by visible...

Subclinical mastitis (SCM) caused by erythromycin-resistant Staphylococcus aureus is a significant disease in lactating animals. Therefore, it crucial to understand the genetic factors contributing erythromycin resistance S. aureus. This study aimed estimate prevalence of milk from subclinical mastitic cattle and buffaloes tank samples as identified probe-based real-time polymerase chain reaction (PCR) genotypic assessment macrolide profiles, well analyze phylogenetic relatedness our local...

Abstract The aim of this study was to examine the genetically mediated antimicrobial resistance in 94 coagulase‐negative staphylococcal ( CNS ) milk isolates (buffalo, n = 88, and cow, 6), determine whether profiles differed between bacterial species. Our analysis from confirmed well‐established multiresistant character staphylococci dairy setting. Resistance against oxacillin, ciprofloxacin cefoxitin most frequently observed. Eleven species isolated buffalo's cow's samples were 100%...

This study was devoted to elucidate the tetracycline resistance of coagulase-negative staphylococci (CNS) derived from normal and subclinical mastitic (SCM) buffaloes' milk in Egypt.A total 81 samples 46 buffalo 35 SCM at private dairy farms Egypt were used this study. CNS identified using phenotypic molecular methods (polymerase chain reaction [PCR]). isolates tested for routine multiplex PCR targeting (tet) genes followed by sequencing positive products phylogenetic analysis.Isolation...

Abstract The breakthrough infection following COVID-19 vaccination has been a subject of concern recently. Evidence suggests that vaccine efficacy diminishes over time due to multiple factors related the host, and vaccine. Coinfection with other pathogens was claimed earlier as contributing cause for this phenomenon. Hence, we aimed stratify association post-COVID-19 coinfection Toxoplasma gondii ( T. ) its impact on disease severity. This cross-sectional study included 330...

Toxoplasmosis is one of the most common parasitic zoonoses that affects all vertebrates. The drugs commonly used against toxoplasmosis have many side effects, making development new antiparasitic a big challenge. present study evaluated therapeutic effectiveness novel herbal treatments, including propolis and wheat germ oil (WGO), acute toxoplasmosis. A total 50 albino mice were divided into five groups: group 1 (G1) (non-infected non-treated); 2 (G2) (infected without treatment); 3 (G3)...

PCR was applied for the discovery of adulteration crude and processed beef meat with horse donkey tissue. This performed by blending (w/w) or to in an extent up 1:10000 (0.01%). The sensitivity resolved as high 0.01%. All used primers showed specificity reactions utilizing layout DNAs from three animal species. application on 96 product samples gathered randomly street vendors prominent retail markets (24 burger, 16 minced meat, 24 kofta, sausage, 7 raw 9 launcheon) uncovered 6 positive...

Background and Aim: Mastitis is an important threat facing goat milk industry the most common cause of culling.Efficient control mastitis, based on efficient diagnosis diseased animals, would improve production reproductive efficiency.In subclinical mastitis (SCM), infected goats demonstrate neither udder symptoms nor abnormal milk.Corynebacterium pseudotuberculosis infectious causative agent mostly results as extension infection from supramammary lymph node, causes financial losses in...

Background and Aim: Campylobacteriosis is one of the most well-characterized bacterial foodborne infections worldwide that arise chiefly due to consumption foods animal origin such as poultry, milk, their products. The disease caused by numerous species within genus Campylobacter, but Campylobacter jejuni commonly isolated from established cases human campylobacteriosis. This study was conducted determine prevalence virulence isolates human, chicken, milk products in Egypt. Materials...

Because of its high case fatality rate, listeriosis locates among the most frequent causes death due to food-borne illness. In this study, a total 150 processed meat samples were collected from Giza Governorate, Egypt. Phenotypic and genotypic identification Listeria monocytogenes was performed using PCR incorporating listeriolysin O virulence gene hlyA followed by DNA sequence analysis. L. confirmed in 4% each beef burger, minced meat, luncheon samples. Phylogenetic analysis showed that all...

Two hundred broiler chicken samples, 160 laying samples and 75 human stool were collected in Egypt. The microbiologically examined, the C. jejuni isolates confirmed biochemically by PCR targeting mapA gene of jejuni. prevalence cadF virulence was then determined using PCR. A total 17.33%, 17% 11.87% stool, respectively, positive for jejuni, with a 66 Campylobacter (15.17%). Ten (15.15%) carried (7.69%, 20.58% 10.52% respectively). Phylogenetic investigation demonstrated that two from had...

Q fever is a zoonotic disease caused by Coxiella burnetii. Cattle, sheep, and goat are the main reservoir of C. In Egypt, epidemiological data about burnetii in camels limited. Therefore, current study was conducted to identify infection different molecular tools estimate its seropositivity through detection anti-C. antibodies camel sera.Blood samples were collected 112 from Giza Cairo Provinces, Egypt. All blood screened trans-quantitative polymerase chain reaction (trans-qPCR) for positive...

Meat samples of various animal species have been obtained from Giza Governorate's butcher shops, common retail markets, veterinary faculties, Zoo and hospitals. Physical, bacteriological, molecular analysis was carried out on the meat samples. Physical examination showed that typical untypical differed. Bacteriological highest TCC found in horse while least goat meat. The total count S. aureus donkey dog whilst pork As for E.coli, buffalo mutton were lowest. Listeria monocytogenes Salmonella...